Soebarinoto's research while affiliated with Brawijaya University and other places

Publications (17)

Article
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Ten rice varieties were planted at two locations (lowland and highland), during the wet and dry seasons of different years. In vivo digestibility and voluntary intake of the straw, were determined in groups of fat-tail sheep, supplemented with 18 g·kg-0.75 concentrate DM, containing ∼20% crude protein. Voluntary intake of digestible straw organic m...
Article
Repair of a double-strand break (DSB) was investigated by intermolecular recombination in Escherichia coli (Ec) recBC sbcBC cells with restriction enzyme-cleaved model plasmids. Circular plasmids were generated when a linearized plasmid (vector) containing an origin of replication was co-transformed with a DNA fragment (template) containing a homol...
Article
Variation in nutritive quality between morphological components is less for rice straw than for wheat straw. Wheat straw stems have a lower quality than stems of rice straw, while leaves and leaf sheaths of wheat are of better quality than of rice. Variation in voluntary organic matter intake, and in vivo and in sacco organic matter digestibility w...

Citations

... Recombineering is a genetic and molecular biology technique based on homologous recombination systems, which mediate e cient recombination of linear DNA molecules anked by long HR [18]. The signi cance of long HR is highlighted by the nding that the targeting cassette with long HR (200-500 bp) is more e cient for recombineering than the targeting cassette with short HR (approximately 50 bp) [11,19,20]. Targeting vectors with long HR were linearized by restriction digestion and puri ed in preparation for subsequent use in BAC recombineering steps. ...
... Values for time lag before the start of degradation observed in the dry season (4.40 and 4.69 h) were similar to those estimated by Soebarinoto et al. (1997) which ranged between 4.51 and 5.68 h for ammoniated straw incubated in sheep fed barley straw. Decreased lag time during the rainy season is attributable to improved rumen environment which led to higher rate of colonisation of substrate by rumen microbes, hence earlier initiation of the degradation process. ...
... These reductions in DMD may be due to that the leaves plus sheaths fraction contained more silica and shorter fibres than the internodal sections (Rizzato and Knight, 1996;Mckean and Jacobs, 1997), leaves plus sheaths also contained insoluble ash and less fibre content, high levels of fermentable sugars, lowest NDF and volatile compounds, which may have been lost with the increases of time and may have lead to decrease of degradability. Leaves tend to be less digestible than stems which is comparable to the reports by many authors (Capper et al., 1988;Khush et al., 1988;Nakashima and Ørskov, 1990;Soebarinoto et al., 1991;Vadivelloo and Fadel, 1992;Karunanandaa et al., 1995.). The DM degradability of leaves plus sheaths of wheat straw at 92h of incubation showed great improvement, which agreed with Givens (1987) who revealed that leaves were more digestible in vitro than stems and the case of UK straw stem height was the major determinant of leaf: stem ratio, short stems being favoured. ...