Sine Yaganoglu's research while affiliated with ETH Zurich and other places

... In the coming years, I suggest that studying organ-specific mechanosensory proteins will likely be a key focus of future investigations, as different organs may employ distinct sets of mechanosensors to sense and respond to fluid flow. Integrating multidisciplinary approaches, including novel ex vivo or 3D in vitro models, structural biology, and live imaging of mechanical stimuli using sensors (Yaganoglu et al., 2023), will contribute to unravelling how proteins can change conformation to sense fluid flows and their associated signalling pathways, and how these changes are interpreted by the cell to effect developmental outputs. ...

... 73,74 Optical imaging with NLO could be performed based on both endogenous contrast (autofluorescence-TPEF, higher-harmonic generation-SHG, vibration energy of interatomic bonds in molecules-CARS/ SRS), as in the vast majority of reported studies, but also based on exogenous agents specifically developed for NLO imaging. 78 In the design presented in Fig. 5, we propose the use of two NLO detection paths, one based on a Gradient-Index (GRIN) lens objective deployable via the functional channel of the endoscope, and a second one based on a liquid tunable lens and a rotating mirror, enabling the probing of NLO signals via a circular "window" on the endoscope body, allowing thus access to GIT lesions positioned in anatomically difficult locations, geometrically unavailable to the GRIN lens objective. The third important feature of GastroAce would stand in its capability for automated diagnostics. ...

... Furthermore, other mechanosensor channels, such as Trpv4, show enrichment in the AV region 50 . Therefore, it would be interesting to generate fluorescent fusion lines of the mechanosensors essential for cardiac valve formation in order to track their trafficking within endocardial cells and study the signals required for their activation 51 . ...

... Clinically approved drugs that inhibit HCVp offer reliable system control and moderate temporal resolution, without undesirable side effects. A non-protease-based optogenetic technique termed PhoCl (PhotoCleavable protein) based on violet-light-induced β-elimination of a photoconvertible fluorescent protein also showed reliable photo-induced cleavage with a wide dynamic range 29,30 . However, to the best of our knowledge, no optically controllable protease-based system comprising a single-component has yet been developed. ...