Shubin Li's research while affiliated with Harbin Institute of Technology and other places

Publications (7)

Article
Full-text available
Constructing a synthetic community system helps scientist understand the complex interactions among species in a community and its environment. Herein, a two-species community is constructed with species A (artificial cells encapsulating pH-responsive molecules and sucrose) and species B (Saccharomyces cerevisiae), which causes the environment to e...
Article
The bottom‐up constructed artificial cells help to understand the cell working mechanism and provide the evolution clues for organisms. The energy supply and metabolism mimicry are the key issues in the field of artificial cells. Herein, an artificial cell containing cyanobacteria capable of light harvesting and carbon dioxide fixation is demonstra...
Preprint
The bottom-up constructed artificial cells help to understand the cell working mechanism and provide the evolution clues for organisms. Cyanobacteria are believed to be the ancestors of chloroplasts according to endosymbiosis theory. Herein we demonstrate an artificial cell containing cyanobacteria to mimic endosymbiosis phenomenon. The cyanobacter...
Article
Full-text available
In nature, cells self-assemble into spatially coded tissular configurations to execute higher-order biological functions as a collective. This mechanism has stimulated the recent trend in synthetic biology to construct tissue-like assemblies from protocell entities, with the aim to understand the evolution mechanism of multicellular mechanisms, cre...
Article
Full-text available
The asymmetric micro/nano motors have attracted great attentions recently, because they convert external physical and chemical energy into the kinetic energy. Red blood cell (RBC) membranes with good deformability and fluidity are excellent natural templates for micromaterial fabrication. Herein, RBC membranes were used as templates to prepare bowl...
Article
The chemical signal communication among organelles in the cell is extremely important for life. We hereby demonstrated that the chemical signal communication between two protoorganelles using cascade enzyme reactions in a lipid based artificial cell. Two protoorganelles inside the artificial cell are the large unilamellar vesicles containing glucos...
Article
Dyestuffs and heavy metal ions in water are seriously harmful to the ecological environment and human health. Three-dimensional (3D) flowerlike Fe(OH)3 microspheres were synthesized through a green yet low-cost injection method, for the removal of organic dyes and heavy metal ions. The Fe(OH)3 microspheres were characterized by thermal gravimetric...

Citations

... Artificial cells are cell-like structures that mimic partial/whole cell structure and functions. These cells have been prepared using natural or synthetic materials 12-14 and are used to clarify the working mechanism of cells [15][16][17][18][19][20][21][22] . Through the secretion and recognition of diffusive signalling molecules in their local environment, artificial cells/live cells in synthetic communities can actively communicate with each other and their surrounding environment to realise critical dynamic biological behaviours, such as predation 23 , protein expression 24,25 , motility 26,27 , quorum sensing 28-30 , and differentiation 31,32 . ...
... The application of an alternating current proposed by Anglova can promote the hydration and swelling of phospholipid membrane [14,53]. The phospholipid membrane layer should be uniformly deposited on special electrode surfaces, such as Pt wire [55], indium tin oxide (ITO) electrode [56,57] and stainless steel electrode [58]. Giant unilamellar vesicles (GUVs) with diameters in the range of 5-200 μm were prepared using an alternating current (frequency: 10 Hz; voltage of 1-7 V; time: 2 h) produced by the electrodes submerged in an aqueous solution [59]. ...
... Shape can dictate propulsion by, for example, trapping the catalyst in a cavity with only one opening, forcing the propelling force through this one outlet [14][15][16][17]. Other methods of dictating the location of propulsion force are through concave shapes [6,18], as oversaturation is more easily reached there, or by differences in surface roughness, since roughness enables bubble pinning and can thus enhance the speed of bubble propelled motors [3,7]. Asymmetry in catalyst distribution directly dictates the location of the propulsion force, since the location of the catalyst is where the reaction happens and, thus, where the propelling products are formed. ...
... 47 To generate hydrogen peroxide we used glucose oxidase. 48,49,50 One population of GUVs, marked with pre-expressed mCherry, expresses αHL with the K3 loop insert and also contains glucose oxidase (sender cells). A second population of GUVs expresses αHL with the E3 loop insert and contains Hyper7 (receiver cells). ...
... The thermal decomposition of Fe mainly occurs between 50 and 500 °C (Fig. 1). These losses are related to the dehydration and dehydroxylation reactions near 150 and 360 °C, respectively [25]. After 500 °C, the total weight loss of Fe substrate was about 24.8%, which is in good agreement with the theoretical calculation (Δm = − 25.2%) from Eq. (2). ...