Shoko Ohshima's research while affiliated with Ibaraki University and other places

Publications (5)

Article
Full-text available
Endohyphal bacteria (EHB), dwelling within fungal hyphae, markedly affect the growth and metabolic potential of their hosts. To date, two EHB belonging to the family Burkholderiaceae have been isolated and characterized as new taxa, Burkholderia rhizoxinica (HKI 454T) and Mycoavidus cysteinexigens (B1-EBT), in Japan. Metagenome sequencing was recen...
Article
Full-text available
An endohyphal bacterium (strain B1-EBT) living in association with the fungus Mortierella elongata FMR23-6 I-B1 was isolated from the fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EBT required cysteine for g...
Article
Full-text available
The fungus Mortierella elongata FMR23-6 harbors an endobacterium inside its mycelium. Attempts to isolate the endobacterium from the fungus were not yet successful, but a highly purified bacterial fraction was prepared. Here, we report the draft genome sequence of the endobacterium. Copyright © 2014 Fujimura et al.

Citations

... Bacterium Pseudomonas fluorescens was recorded to stimulate both colonization and growth of the fungus Laccaria bicolor (Deveau et al., 2007). In a mutual interaction, bacteria consume metabolites from the fungus and contribute amino acids in return (Lackner et al., 2011) or benefit of carbon and nitrogen exchange (Sharmin et al., 2018). The symbiont could also help the fungus (Guo et al., 2017) or bacteria (Ruiz-Lozano and Bonfante, 2000) to establish or facilitate invasion to other fungi (Spraker et al., 2016) or plant tissues (Ruiz-Lozano and Bonfante, 2000). ...
... Additionally, Mycoavidus cysteinexigens sp. nov, the endosymbiont of Mortierella elongata, along with several strains of bacteria from the family Burkholderiaceae were reported to be unable to utilize maltose (Zhang et al., 2000;Ohshima et al., 2016) suggesting the Mycoavidus examined here may be metabolically restricted and reliant on host metabolism, thus slowing growth. Vogel's media in this study was associated with increased autofluorescence making automated quantitation difficult, but qualitatively still exhibited endobacterial growth. ...
... So far, seven fungal endobacteria genomes have been published, and they are Candidatus Glomeribacter gigasporarum colonizing arbuscular mycorrhizal fungi (AMF) Gigaspora margarita (Ghignone et al., 2012); Burkholderia rhizoxinica colonizing Rhizopus microsporus, which participates in the production of rhizoxin (Lackner et al., 2011); a betaproteobacterial endosymbiont associated with Mortierella elongate (Fujimura et al., 2014), mycoplasma-related endobacteria (MRE) living in Rhizophagus clarus, MRE living in Racocetra verrucosa, MRE living in Claroideoglomus etunicatum (Naito et al., 2015), MRE living in AMF Dentiscutata heterogama (Torres-Cortés et al., 2015). ...