Shiyu Sun’s scientific contributions

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Publications (1)


Alpha diversity analysis (A, B) and rarefaction curves (C) of the different groups. (**0.001 < P ≤ 0.01, ***P ≤ 0.001).
The mean relative abundances of bacterial phyla (A) and genera (B). (C) Venn diagram of the different groups. (D) Shared microbial genera across three groups. (E–G) Unique microbial genera in each group.
Microbiota typing analysis on genus level (A) and the microbiota types composition within each group (B), Heatmap of the abundance of the first 30 bacterial genera and the sample clustering tree (C).
KEGG pathway classification (A) and top 20 KEGG pathway (B) for all samples.
(A–C) Up-or down-regulated metabolites in different diet groups. (D–F) The Pls-DA score plot showed separation degree of the two groups (group A VS B, group B VS C, group A VS C). (G–I) For the validation of the above PLS-DA models, the number of random permutation tests is 200.

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Combined analysis of the microbiome and metabolome to reveal the characteristics of saliva from different diets: a comparison among vegans, seafood-based omnivores, and red meat (beef and lamb) omnivores
  • Article
  • Full-text available

July 2024

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27 Reads

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1 Citation

Shiyu Sun

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Huiqiong Zhang

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Linying Ye

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[...]

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Introduction Revealing individual characteristics is supportive for identifying individuals in forensic crime. As saliva is one of the most common biological samples used in crime scenes, it is important to make full use of the rich individual information contained in saliva. The aim of this study was to explore the application of the microbiome in forensic science by analysing differences in the salivary microbiome and metabolome of healthy individuals with different dietary habits. Methods We performed 16S rDNA sequencing analysis based on oral saliva samples collected from 12 vegetarians, 12 seafood omnivores and 12 beef and lamb omnivores. Non-targeted metabolomics analyses were also performed based on saliva samples from healthy individuals. Results The results showed that the dominant flora of vegetarians was dominated by Neisseria (belonging to the phylum Proteobacteria), while seafood omnivores and beef and lamb omnivores were dominated by Streptococcus (belonging to the phylum Firmicutes). NDMS-based and cluster analyses showed that vegetarian dieters were significantly differentiated from meat dieters (seafood omnivores and beef and lamb omnivores), which may be related to the fact that high-fiber diets can create a different salivary flora structure. Variants were also detected in salivary metabolic pathways, including positive correlations with Lipid metabolism, Amino acid metabolism, Carbohydrate metabolism, and Nucleotide metabolism in vegetarians, and correlations in seafood omnivores. In order to select salivary microorganisms and metabolic markers that can distinguish different dietary profiles, a random forest classifier model was constructed in this study, and the results showed that individuals with different dietary profiles could be successfully distinguished based on the core genera and metabolites such as Streptococcus, Histidinyl-Valine. Conclusion Our study provides a supportive basis for the application of salivary polyomics in order to reveal the dietary characteristics of individuals for forensic investigation and crime solving.

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Citations (1)


... Firmicutes have been thought to have a role in obesity as obese people tend to have proportionally more Firmicutes to Bacteroidota than lean people, and a low-calorie diet and weight loss decreases Firmicutes and increases Bacteroidota [47]. Diet has also been shown to affect host bacterial composition, as people eating higher fat and protein diets have more Firmicutes than those with a diet richer in fiber and vegetables [48,49]. In fact, the diet itself, even without changes in body weight, affects the bacterial composition of the host; mice fed with a high-fat diet had an increase in Firmicutes with and without concomitant obesity [50]. ...

Reference:

Regular Exercise Training Induces More Changes on Intestinal Glucose Uptake from Blood and Microbiota Composition in Leaner Compared to Heavier Individuals in Monozygotic Twins Discordant for BMI
Combined analysis of the microbiome and metabolome to reveal the characteristics of saliva from different diets: a comparison among vegans, seafood-based omnivores, and red meat (beef and lamb) omnivores