Shaun H. Preston’s research while affiliated with Creo Medical and other places

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Publications (1)

A schematic illustrating the stages of varicose vein disease. Varicose vein formation, classification, and progression: (A) C0, absence of visible venous disease signs; (B) C0-C1: C1, vessel wall dilation, and blood pooling which support leaflet deformation and the manifestation of telangiectasias or reticular veins, and C2, presence of varicose veins; and (C) C3-C6: C3, development of edema and enhanced luminal expansion and leaflet damage. Additional aspects of this disease progression (not included in this schematic) include C4, the occurrence of pigmentation or eczema and the emergence of lipodermatosclerosis or atrophy blanche; C5, a healed ulcer; and C6, an active ulcer. Moreover, early-stage varicose veins are present in stages C1-C2, and stages C3 to C6 are categorized as CVI, indicative of the venous disease’s advanced clinical stage.
Functional hydrogel formulation. The functional hydrogel can be devised by (A) extracting vascular tissues from cadaveric sources, (B) decellularizing the valves to create dECM, and (C) this dECM can then be lyophilized, ground into powder, digested with pepsin/HCl digested and neutralized to form the hydrogel. Simultaneously, (D) extraction of adipose tissues, (E) provides a source of adipose-derived stem cells (ADSCs) that can be (F) differentiated into ECs, SMCs, and VICs. After that, (G) the tissue-specific hydrogel can be enriched with the various cell types and gold nanoparticles (AuNPs).
An illustration of image-guided hydrogel infusion for valvular enhancement. The diagram depicts the (A) ultrasound and/or (B) C-arm systems guiding catheter insertion into various valvular regions for hydrogel infusion into the wall and leaflets. (C) The hydrogel, composed of dECM, differentiated cells, and AuNPs, provides a medium matching the native microenvironment that mechanical forces and growth factors can aid valvular restoration and regeneration. (D) Within the infused dECM hydrogel, ECs, SMCs, and VICs contribute to collagen and elastin synthesis, re-endothelialization, and reconstitution of damaged tunics. (E) AuNPs counteract MMP activity, curtailing ECM degradation, vessel wall distension, and the potential propensity to halt disease progression.
From the slaughterhouse to the lab: extracting natural biomaterials from substantial volumes of common livestock (cows and sheep) during meat processing. (A) Discarded abdominal tissues collected during cow meat processing from which adipose tissues can be extracted. (B) Our expert team shown dissecting tissues to isolate intact vascular substrates. Vascular tissues extracted from the original discarded mass (C) before and (D) after lipectomy to collect perivascular adipose tissue. (E) Extracted adipose tissues after being washed thoroughly with a sterile solution to remove blood and debris, allowing for stem cell extraction. (F) Decellularized tissues that can be used to generate hydrogels.
Sustainable varicose vein therapy using functionalized hydrogels derived solely from livestock waste
  • Article
  • Full-text available

August 2024

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100 Reads

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Meklit G. Shibru

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Shaun H. Preston

Developing sustainable and effective treatments for chronic venous insufficiency (CVI) is crucial. In this study, we propose an innovative restorative approach utilizing hydrogels derived from the decellularized extracellular matrix (dECM) of cadaveric vascular tissues, adipose-derived stem cells (ADSCs), and gold nanoparticles (AuNPs). This therapeutic method leverages waste valorization by repurposing discarded cadaveric tissues from slaughterhouse livestock. The dECM hydrogels, enriched with ADSCs and AuNPs, offer a biocompatible scaffold that supports cellular differentiation and vascular integrity. Our approach addresses the limitations of current allo-, auto-, and xenograft methods by enhancing integration and functionality while potentially reducing costs through sustainable practices. This study explores functionalized hydrogel formulation solely generated from agri-food waste, gelation mechanisms, and preliminary cost-effectiveness, presenting a promising new avenue for treating early-stage varicose veins that can ultimately be translated to human models using discarded tissues.

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