Shaocheng Chen’s research while affiliated with Chongqing University and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (6)


Corrigendum to “Cynapanoside A exerts protective effects against obesity-induced diabetic nephropathy through ameliorating TRIM31-mediated inflammation, lipid synthesis and fibrosis” [Int. Immunopharmacol. 113 (Part B) (2022) 109395]
  • Article

October 2023

·

2 Reads

International Immunopharmacology

Jing Luo

·

·

Junjie Zhao

·

[...]

·


Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H 2 O 2
  • Article
  • Full-text available

September 2023

·

9 Reads

Experimental and Therapeutic Medicine

Peucedanum praeruptorum Dunn extract (PPDE) is a well-known treatment used in traditional Chinese medicines, where it is most commonly used to treat coughs and symptoms such as headaches and fever. In the present study, the antioxidant capacity of PPDE in vitro was determined by scavenging experiments using DPPH, ABTS⁺·, ·OH, and ·O2⁻. The cell survival rate was determined by MTT assay. The MDA, SOD, CAT, GSH, and GSH-Px content were determined by colorimetry assays. The expression levels of antioxidant genes SOD, CAT, GSH, and GSH-Px were assessed by reverse transcription-quantitative PCR. HPLC was used to identify the PPDE components. The results suggested that PPDE had scavenging effects on DPPH, ABTS, hydroxyl, and superoxide anion radicals in a concentration-dependent manner; H2O2 treatment resulted in oxidative stress in LLC-PK1 cells, and the degree of injury of LLC-PK1 cells following PPDE treatment was improved, which was positively correlated with its concentration. Peucedanum praeruptorum Dunn extract treatment reduced the content of MDA and increased the content of CAT, SOD1, GSH, and GSH-Px. The mRNA expression levels of antioxidant genes detected by quantitative PCR were consistent with changes in CAT, SOD, GSS, and GSH-Px. Additionally, the trend in CAT, SOD1, GSH, and GSS protein expression levels was also consistent at the mRNA level. PPDE was found to consist of isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol. Therefore, PPDE, which was formed of products derived from natural substances, functioned in the inhibition of oxidative damage. The present study aimed to obtain a better understanding of the traditional Chinese medicine Peucedanum praeruptorum Dunn and preliminarily elucidate its antioxidant mechanism at the cellular level. Further animal or human experiments are required to verify the antioxidant effects of PPDE for further development and utilization.

Download

Compound composition of the Peucedanum praeruptorum Dunn extract (PPDE) detected by HPLC.
Effects of Peucedanum praeruptorum Dunn extract (PPDE) and positive control drug of doxorubicin on proliferation of the human normal liver cell line L02 and HepG2 human hepatoma cells (n = 8). (a) PPDE-treated L02 cells, (b) PPDE-treated HepG2 cells, (c) doxorubicin-treated L02 cells, and (d) doxorubicin-treated HepG2 cells.
Effects of Peucedanum praeruptorum Dunn extract (PPDE) and positive control drug of doxorubicin on proliferation of the human normal liver cell line L02 and HepG2 human hepatoma cells (n = 8). (a) PPDE-treated L02 cells, (b) PPDE-treated HepG2 cells, (c) doxorubicin-treated L02 cells, and (d) doxorubicin-treated HepG2 cells.
Effects of Peucedanum praeruptorum Dunn extract (PPDE) and positive control drug of doxorubicin on proliferation of the human normal liver cell line L02 and HepG2 human hepatoma cells (n = 8). (a) PPDE-treated L02 cells, (b) PPDE-treated HepG2 cells, (c) doxorubicin-treated L02 cells, and (d) doxorubicin-treated HepG2 cells.
Effects of Peucedanum praeruptorum Dunn extract (PPDE) and positive control drug of doxorubicin on proliferation of the human normal liver cell line L02 and HepG2 human hepatoma cells (n = 8). (a) PPDE-treated L02 cells, (b) PPDE-treated HepG2 cells, (c) doxorubicin-treated L02 cells, and (d) doxorubicin-treated HepG2 cells.

+8

In Vitro Investigation of the Anticancer Activity of Peucedanum praeruptorum Dunn Extract on HepG2 Human Hepatoma Cells

September 2023

·

35 Reads

·

1 Citation

The anticancer activity of Peucedanum praeruptorum Dunn extract (PPDE) was investigated in vitro in the HepG2 human hepatoma cell line and compared to normal human liver cells (L02 cells). The effect of the PPDE on the proliferation of the cells was measured by MTT assays, and the levels of enzymes and small molecules implicated in oxidative stress regulation were measured using specific reagent kits. The expression levels of genes implicated in apoptosis (Bax, Bcl-2, caspase-3, caspase-8, and caspase-9) and oxidative stress (SOD1 and SOD2) were quantified by RT-qPCR. Lastly, HPLC was employed to analyze the composition of the PPDE. PPDE was found to significantly inhibit the proliferation of HepG2 cells but had little effect on the proliferation of normal liver cells. PPDE increased the levels of reactive oxygen species and malonaldehyde, a lipid peroxidation product, in HepG2 cells, and it reduced the activities of antioxidant enzymes, as well as the levels of γ-GCS and reduced glutathione (GSH), suggesting that it inhibited the ability of cancer cells to regulate intracellular oxidative stress. PPDE also increased the expression of the genes encoding Bax, caspase-3, caspase-8, and caspase-9 and decreased the expression of Bcl-2, SOD1, and SOD2 in HepG2 cells, suggesting that PPDE induced the apoptosis of the liver cancer cells. HPLC analysis identified that the components of PPDE included caffeic acid, isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol, all of which have demonstrated antioxidant properties.


Cynapanoside A exerts protective effects against obesity-induced diabetic nephropathy through ameliorating TRIM31-mediated inflammation, lipid synthesis and fibrosis

December 2022

·

13 Reads

·

3 Citations

International Immunopharmacology

Obesity is a major predictive factor for the diabetic nephropathy (DN). However, the precise mechanism and therapeutic approach still require to be investigated. Cynapanosides A (CPS-A) is a glycoside derived from the Chinese drug Cynanchum paniculatum that has numerous pharmacological activities, but its regulatory function on obesity-induced kidney disease is still obscure. In the present study, we attempted to explore the renoprotective effects of CPS-A on the established DN in high fat diet (HFD)-fed mice, and the underlying mechanisms. We initially found that CPS-A significantly ameliorated the obesity and metabolic syndrome in mice with HFD feeding. Mice with HFD-induced DN exerted renal dysfunctions, indicated by the elevated functional parameters, including up-regulated blood urea nitrogen (BUN), urine albumin and creatinine, which were significantly attenuated by CPS-A in obese mice. Moreover, histological changes including glomerular enlargement, sclerosis index and collagen deposition in kidney of obese mice were detected, while being strongly ameliorated by CPS-A. Additionally, podocyte loss induced by HFD was also markedly mitigated in mice with CPS-A supplementation. HFD feeding also led to lipid deposition and inflammatory response in renal tissues of obese mice, whereas being considerably attenuated after CPS-A consumption. Intriguingly, we found that tripartite motif-containing protein 31 (TRIM31) signaling might be a crucial mechanism for CPS-A to perform its renoprotective functions in mice with DN. The anti-inflammatory, anti-fibrotic and anti-dyslipidemia capacities of CPS-A were confirmed in the mouse podocytes under varying metabolic stresses, which were however almost abolished upon TRIM31 ablation. These data elucidated that TRIM31 expression was largely required for CPS-A to perform its renoprotective effects. Collectively, our study is the first to reveal that CPS-A may be a promising therapeutic strategy for the treatment of obesity-induced DN or associated kidney disease.


Figure 1. Peucedanum praeruptorum Dunn form and its application in food industry.
Effect of functional food raw material Peucedanum praeruptorum Dunn: a research update

July 2022

·

37 Reads

·

2 Citations

Food Science and Technology (Campinas)

Abstract Peucedanum praeruptorum Dunn is a natural plant with expectorant, antitussive, anti-asthmatic, anti-inflammatory, antispasmodic and sedative effects. It is used in traditional Chinese medicine. The key active substances extracted after separation are also used to process functional food. Recent advances in the development of Peucedanum praeruptorum Dunn industrially have increased the market demand. In particular, the study of the physiological activity of P. praeruptorum Dunn has always been a research hotspot; however, limited data exist currently. This study reviews the research developments associated with the physiological activities of P. praeruptorum Dunn in terms of cardiovascular, expectorant and antitussive, antitumor, and anti-inflammatory effects. The results serve as a standard of reference for further investigation into the pharmacologic role of Peucedanum praeruptorum Dunn.


Citations (2)


... Abnormal expression of Trim31 has been observed in a variety of pathological conditions, such as cancer, metabolic-associated fatty liver disease, ischemic stroke, and hypertensive nephropathy [9][10][11][12]. Notably, Trim31 possesses anti-inflammatory properties and is closely associated with a range of inflammation-related ailments [13][14][15]. Trim31 may exhibit various functions in different contexts. Therefore, the role of Trim31 in different physiological and pathological processes is worthy of investigation. ...

Reference:

Loss of Trim31 Worsens Cardiac Remodeling in a Mouse Model of Heart Failure by Enhancing the Activation of the NLRP3 Inflammasome
Cynapanoside A exerts protective effects against obesity-induced diabetic nephropathy through ameliorating TRIM31-mediated inflammation, lipid synthesis and fibrosis
  • Citing Article
  • December 2022

International Immunopharmacology

... 42 Moreover, Zi Hua Qian Hu was known for antitussive and expectorant effects, which were enhanced after honey-frying and had been demonstrated in in vivo test. 43 Radix et Rhizoma Asteris: Tatarinow's aster, Zi Wan (Chinese: 紫菀), Tử uyển (Vietnamese) Processing. Refined honey (ratio 1:4 to herb ingredient, w/w) is diluted with water (ratio 4:1 to honey) and then mixed well with sliced Zi Wan. ...

Effect of functional food raw material Peucedanum praeruptorum Dunn: a research update

Food Science and Technology (Campinas)