Shania Fang’s research while affiliated with Cornell University and other places

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Publications (1)

FIG 1 Performance of M. album BG8 and M. parvus OBBP reactors. (A) M. album BG8, 2.8-day RT, CH 4 limitation, transition to 5.7-day RT; (B) M. album BG8, 4.3-day RT, O 2 limitation, transition to 9.1-day RT; (C) M. parvus OBBP, 2.8-day RT, 5.8-day RT; (D) M. parvus OBBP, 4.3-day RT, 9.0-day RT. Aqueous concentrations of CH 4 and O 2 , left y axis, and biomass, right y axis. Data are means 6 standard deviations from triplicate reactors.
FIG 2 Steady-state per-cell pmoA transcript amounts and methane oxidation rates for aerobic methanotrophs. Data are averages from individual reactors from distinct sampling dates. Error bars represent standard deviations of biomarker amounts (y axis) from replicate reactors. Power law trend and R 2 value are shown. M. trichosporium OB3b data were obtained from the work of Tentori and Richardson (37).
FIG 3 M. album BG8 reactors during substrate limitation and recovery. (A) 2.8-day RT reactor response during CH 4 limitation; (B) 2.8-day RT reactor genome and pmoA mRNA amounts during CH 4 limitation; (C) 2.8-day RT reactor pmoA mRNA copies per cell during CH 4 limitation; (D) 4.3-day RT reactor response during O 2 limitation; (E) 4.3-day RT reactor genome and pmoA mRNA amounts during O 2 limitation; (F) 4.3-day RT reactor pmoA mRNA copies per cell during O 2 limitation. Data are means 6 standard deviations from triplicate reactors. Asterisks indicate samples selected for RNA-seq. Data following RT switch are not shown.
FIG 4 Classification of significant differential gene expression (DGE) in M. album BG8, based on COG classification from the eggNOG 5.0 database. Positive and negative values on the x axis indicate numbers of genes in that COG category that were upregulated and downregulated, respectively, compared to steady-state conditions. Unclassified, category S ("Function Unknown") and categories with fewer than two genes with DGE are not shown.
FIG 5 Changes in central metabolic pathways in M. album BG8 during short-term substrate limitation of CH 4 (top) and O 2 (bottom). Upregulated (green) and downregulated (purple) pathways and pathways with no change (gray) compared to steady-state conditions are highlighted. Multiple colors indicate that different genes in that pathway were both up-and downregulated. Abbreviations and corresponding intermediates and compounds are provided in Table S7.

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RNA Biomarker Trends across Type I and Type II Aerobic Methanotrophs in Response to Methane Oxidation Rates and Transcriptome Response to Short-Term Methane and Oxygen Limitation in Methylomicrobium album BG8
  • Article
  • Full-text available

June 2022

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138 Reads

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8 Citations

Microbiology Spectrum

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Shania Fang

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Ruth E. Richardson

Methanotrophs are naturally occurring microorganisms capable of oxidizing methane, having an impact on global net methane emissions. Additionally, they have also gained interest for their biotechnological applications in single-cell protein production, biofuels, and bioplastics.

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Citations (1)

... Cytochrome c L , in turn, is oxidized by a typical class I cytochrome c (cytochrome c H ), which is also specific for methanol oxidation (Kang-Yun et al. 2022). Importantly, all three components-MDH, cytochrome c L , and cytochrome c H -are soluble and reside within the periplasm of gramnegative methylotrophs (Tentori et al. 2022). In contrast, gram-positive methylotrophs employ an NAD-linked MDH for methanol oxidation, while methanol-oxidizing yeast species use a methanol oxidase system for the same purpose. ...

Reference:

Recent findings in methanotrophs: genetics, molecular ecology, and biopotential
RNA Biomarker Trends across Type I and Type II Aerobic Methanotrophs in Response to Methane Oxidation Rates and Transcriptome Response to Short-Term Methane and Oxygen Limitation in Methylomicrobium album BG8

Microbiology Spectrum

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Shania Fang

·

Ruth E. Richardson