Shalal M Hussain’s scientific contributions

This study was designed to generate hepatocyte cells from mesenchymal stem cells (MSCs) isolated from mouse bone marrow via using culturing protocols for this goal. Cells of MSCs were isolated by collecting the thigh bone of 50 albino mice, both femur and tibia were collected and cells were flushed from bones and MSCs have been isolated based on the ability of MSCs to adhere on plastic surfaces. MSCs reactivity to CD45, CD90, CD34 and CD105 were tested by immunocytochemistry. Isolated MSCs exhibited positive reactivity towars CD105 and CD90 cell surface markers. MSCs were negative for the hematopoietic surface markers including CD34 and CD45. Differentiation into hepatocyte was induced by adding hepatogenic specific growth factors (Hepatocytes growth factors (HGF), Fibroblast growth factor4 (FGF4), oncostatin M and dexamethasone) to the differentiation medium. Cells were examined after differentiation protocol and characterized using immunocytochemistry analysis for Albumin and alpha fetoprotein. Positive results were observed indicating the potential ability of the isolated bone marrow MSCs to differentiate into Hepatocytes.

This study was designed to identify the presence of cytochrome oxidase p450 (CYP3A4), in the hepatic cell lines which was generated from the in vitro differentiation of mouse bone marrow mesenchymal stem cells. Bone marrow meenchymal stem cells were first isolated by collecting both the femur and tibia of the mouse thigh bone, then exess tissue were removed and the cells were flushed from the bones and cultured under highly aseptic conditions. The isolated cells were characterized as a mesenchymal stem cells via using immunocytochemistry analysis. The characterized hepatocytes were subjected to a differentiation protocol in which the differentiation medium was contained a spesfic growth factor including (Hepatocytes growth factor (HGF), Fibroblast growth factor4 (FGF4), Oncostatin M and Dexamethasone, All theses growth factor was used for directing the BM-MSCs twards the hepatic line of cells. After three weeks of the differentiation protocol the diffrentation cells were examined for the presence of Cytochrome oxidase p450 (CYP3A4), which consider as one of the specific markers of hepatocytes. Identification eas done via two different methods first, via using the immunocytochemical analysis for the antihuman mouse CYP3A4 antibody and second by ELIZA test. The results showed a posotive reactivity of the differentiated cells towards the mouse antihuman CYP3A4 antibody as well as the eliza results showed a significant increase in the enzyme level of the cell lysate during the weeks of the differentiation experiment.