Sabine Horn's research while affiliated with Max Planck Institute for Molecular Genetics and other places
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Publications (5)
Dilated cardiomyopathy (DCM) is a myocardial disease characterized by progressive depression of myocardial contractile function and ventricular dilatation. Thirty percent of DCM patients belong to the inherited genetic form; the rest may be idiopathic, viral, autoimmune, or immune-mediated associated with a viral infection. Disturbances in humoral...
The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T(H)1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on th...
Heterologous gene expression is often treated empirically and a number of host organisms are systematically tested. Early successes in the expression of recombinant proteins were achieved using the well-studied bacterium Escherichia coli (1). This prokaryotic expression system is simple to handle, costeffective, and produces large amounts of hetero...
Citations
... The low cost of culture media, the ease of scaling up from small cultures to fermentation cultures, and high biomass production in fermenters (up to 400 g/L) combine to make Pichia a very useful protein expression system. Several thousand heterologous proteins have now been expressed in P. pastoris (Cregg 2007), including many eukaryotic and integral membrane proteins that were nonfunctional when expressed in E. coli (Lueking et al. 2003;Parcej and Eckhardt-Strelau 2003;Long et al. 2005;Andre et al. 2006;Aller et al. 2009); see also Introduction: Considerations for Membrane Protein Purification (Kielkopf et al. 2021g). ...
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... A high-density multiprotein array (MPA) (hEXselect, Engine, Berlin, Germany, Order No. 1003) derived from a cDNA bank of two first trimester human fetal brain samples, containing 23,806 E. coli expression clones representing a total of 10,000 human proteins (Büssow et al. 1998;Horn et al. 2006), was incubated with either α-HPy or α-CJe according to the manufacturer's protocol. In brief, prior to the first use of these arrays, protein spots were fixed on the PVDF membrane for 10 min with 70% ethanol and rinsed twice with distilled water. ...
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... Anti-ribosomal P antibodies are correlated with a specific clinical subtype of SLE, i.e., SLE with neuropsychiatric symptomatology (NPSLE) [27]. Although they have not yet been demonstrated in the serum of SLE patients, autoantibodies to RPS27a have been detected in a mouse model of SLE and therefore could play also a role for this disease [28]. ...
