S.K. Jadhav’s research while affiliated with Pandit Ravishankar Shukla University and other places

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Publications (131)


Proximate analysis of Pleurotus florida influenced by magnesium sulfate (MgSO4) in submerged and solid-state cultivation
  • Article

December 2024

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12 Reads

Research on Crops

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SANDEEP SAHU

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S. K. JADHAV

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Pleurotus florida is a widely consumed and easily cultivated mushroom, valued for its high nutritional content. This study aimed to enhance its mineral content through substrate fortification, specifically using magnesium sulfate, to create more nutrient-rich food products. Since minerals are crucial for mushroom growth, supplementing substrates can significantly improve both incubation and fructification, addressing the need for higher-quality mushrooms in functional foods and dietary supplements. Therefore, an experiment was conducted at the Mushroom Technology Laboratory, School of Studies in Biotechnology, Pt Ravishankar Shukla University, Raipur, India, to examine the effects of Magnesium sulfate (MgSO4) on P. florida during the rainy season (July–October 2022) in both submerged and solid-state cultivation. Different concentration of MgSO4 (Control, 200 mg/kg, 400 mg/kg, 600 mg/kg, 800 mg/kg and 1000 mg/kg) was supplemented to PDA media and paddy straw for cultivation of P.floridamushroom. The study assessed fruiting body production, mycelial biomass yield, protein content, and antioxidant activity. Results showed that MgSO4 significantly increased biomass production (28.67mg/100ml and 89.06g/500g of substrate for T3 for both mycelia and fruiting body), antioxidant activity (88.90 and 96.86% for control), and bioactive content (protein 20.28and 20.72 mg/100g for both mycelia and fruiting body respectively at treatment T4), notably affecting the mushrooms' metabolic profile (p less than 0.05). These findings suggest that mineral-fortified P. florida mushrooms are rich in essential nutrients and have potential as valuable pharmaceutical products.


A Selection of hybrid mycelia through barrage rection. B Distinct and combined characteristics of mycelial cultures from parental strains and their hybrid strain after fusion, highlighting enhanced growth and unique morphology. C Microscopic study of mycelia of parental and hybrid strain through a phase contrast microscope
Effect of IPS and EPS of parental and hybrid strain on DPPH radical scavenging activity
Antimicrobial activity of polysaccharides against gram positive and gram negative bacteria A EPS and B IPS
Identified compounds through GC–MS analysis
FTIR spectra of EPS from mycelia of species C. militaris, P. florida, and hybrid strain
GC–MS Analysis of Polysaccharides from an Intergeneric Hybrid of Pleurotus florida and Cordyceps militaris: A Comparative Study
  • Article
  • Publisher preview available

November 2024

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50 Reads

Applied Biochemistry and Biotechnology

Edible and medicinal mushrooms are valuable sources of polysaccharides, known for their dual roles as immunostimulants and immunosuppressants. This study aimed to enhance polysaccharide content by fusing two mushroom species, P. florida and C. militaris, while exploring their antioxidant and antibacterial potential. These mushrooms have diverse health benefits, including lowering high cholesterol, providing anti-inflammatory effects, supporting diabetes management, aiding in cancer treatment, and enhancing the efficacy of COVID-19 vaccines. Successful hyphal fusion was achieved, and optimal culture conditions were determined using response surface methodology. The hybrids exhibited superior growth compared to the parental strains. Hyphal fusion improved several attributes, resulting in diverse hybrids with increased biomass and metabolite production. FTIR analysis confirmed the presence of exopolysaccharides, with concentrations measured at 28.4 g/L (P1), 31.50 g/L (CD), and 36.74 g/L (F3). GC–MS analysis identified various bioactive metabolites, including a higher concentration of dimethyl palmitamine in the hybrid, a novel compound, butanenitrile, 2-(methoxymethoxy), which was not found in the parental strains. These compounds are likely responsible for the enhanced antimicrobial and antioxidant activities. Graphical Abstract

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Valorization of the Lignocellulosic Biomass via Bacterial Enzymatic Machinery for Sustainable Biorefinery

October 2024

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9 Reads

The lignocellulosic biomass under a “waste to use” program is an intriguing substitute to various nonrecyclable sources for bio-product formation due to its accessibility, abundance, and recyclability, which has attracted significant attention on a global scale. However, the utilization of recalcitrant lignocellulosic biomass to satisfy the booming demand of the ever-increasing population is a bottleneck for biorefineries. Nevertheless, the excellent potential of microorganisms to produce extracellular lignocellulolytic enzymes (hydrolases) via their enzymatic machinery possesses tremendous application for serving a wide range of applications in biorefineries. The bacterial enzymatic machinery hydrolyzes the lignocellulosic feedstocks through the destruction and dissociation of the cross-linked, hierarchical structure within lignin, cellulose, and hemicellulose. The current chapter deals with insights into a benign approach for the utilization of bacterial enzymatic machinery for manufacturing value-added products like biofuels, organic acid, antibiotics, proteins, enzymes, and pigments through the hydrolysis of complex lignocellulosic biomass, which will open a new scope for biorefineries in terms of sustainability and economic feasibility.




Zn Fortification Influential Impact on the Productivity of Calocybe indica Mycelium

December 2023

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125 Reads

Journal of Ravishankar University (Part-B)

Calocybe indica is an edible medicinal mushroom, preferably eaten for its culinary value. It was cultivated for its higher nutritional value, medicinal properties, and high polysaccharide content, especially glucan. Some particular minerals were enriched in food substitutes, an alternative to fighting against some targeted human ailments. Thus, mineral fortification is accessible in the submerged cultivation of mushrooms to produce bioactive compounds and fortified mushrooms. In this study, the submerged cultivation of C. indica was performed to make exopolysaccharides (EPS) using a supplemented medium of Zinc with varying concentrations. Thus, research revealed that the Zn fortification enhances the production of EPS and mycelial biomass after 21 days of incubation. The maximum mycelial biomass was 7.7133±0.30 g/L (dry weight), and the highest 0.3853±0.006EPS was produced in the 175mg/L mineral concentration, respectively. The present study revealed that the Zn supplementation gradually increased the mineral concentration and directly influenced the yield of mycelial biomass and EPS production. These EPS have various biological activities and can be helpful for fortified food or pharmaceutical product development in the medicinal and pharmaceutical sectors.





Citations (63)


... The initial pH was adjusted to the expected value by using 1 N HCl or 1 N NaOH. The reagent bottles were purged with CO 2 to create anaerobic conditions and the bottles were incubated at 28 C. The effect of different pH (5.0, 5.5, 6.0, 6.5 and 7.0) and temperature (20 to 40 C) on hydrogen production was examined [21]. The mass production of hydrogen was examined with optimized fermentation medium (reactor volume of 1500 mL in 2000 mL reagent bottle) and supplemented with 4% sugarcane molasses at pH 7.0 and temperature 30 C. The growth kinetic analysis was performed on the batch hydrogen fermentation treatments using sugarcane molasses at different concentrations (1 to 5%). ...

Reference:

Production and optimization of biohydrogen from saccharolytic actinobacterium, Streptomyces rubiginosus (SM16), using sugarcane molasses
Isolation and Characterization of Biohydrogen Producing Bacteria from Rice Bran with Optimization of Different Parameters

... Optimal results were observed at 37 • C and pH 8.0 over a 96 h incubation period with 1.5% (w/v) maltose. The study indicates that the combined laundry power of detergents and enzymes surpasses their individual effectiveness, highlighting the necessity of α-amylase as a key ingredient in cleaning detergent formulations [141]. The starch was grafted with acrylamide with 2-(methacryloyloxy) ethanol to boost its resistance to abrasion and minimize hairy yarn appearance [3]. ...

Biovalorizing agro-waste ‘de-oiled rice bran’ for thermostable, alkalophilic and detergent stable α-amylase production with its application as laundry detergent additive and textile desizer
  • Citing Article
  • November 2023

International Journal of Biological Macromolecules

... It has also been reported that at high temperatures, the rate of interaction between biomass and heavy metal ions decreases. With increasing temperature, the boundary layer diminishes, resulting in a decreased removal rate of heavy metal ions (Sharma et al., 2023). In general, the best adsorption temperature range of P. lilacinus for heavy metals in electrolytes is 25-30 • C. ...

Lead biosorption profiling of endophytic Aspergillus flavus SGE34 isolated from Cleome viscosa Linn.
  • Citing Article
  • September 2023

Chemistry and Ecology

... Ethanol is one of the most common energy-related fermentation products that can be transformed from a diverse range of low-cost lignocellulosic feedstocks, such as sugarcane bagasse by Kluyveromyces marxianus JKH5 C60 [92], coculture of Saccharomyces cerevisiae and K. marxianus [93], corn stover by Zymomonas mobilis 8b [94], cobs by K. marxianus [95], wheat straw by Pichia kudriavzevii SVMS2019 [96], rice husk by Aspergillus terreus, Trichoderma harzanium [97] and Enterobacter cloacae [98]. However, a major disadvantage associated with lignocellulosic-based feedstock is the generation of hydrolysis byproducts cum fermentation inhibitors. ...

Bioconversion of Rice Husk for Bioethanol Production from a Novel Bacteria Enterobacter Cloacae Nc231221
  • Citing Article
  • January 2023

SSRN Electronic Journal

... Klebsiella pneumoniae is mentioned in one of the provided as a potential host organism for the de novo production of 2butanol, another butanol isomer, through metabolic engineering (Chen et al. 2015). In another study, K. pneumoniae UT3 was used for fermentative ethanol production from Shorea robusta through simultaneous saccharification and fermentation (SSF) (Choudhary et al. 2018). Numerous studies point to Saccharomyces cerevisiae as a suitable host organism for the generation of biofuels because of its complex genetic toolbox, easy modification of metabolic pathways and strain characteristics, and well-established commercial fermentations (Du and Zhao 2011;Khan et al. 2023;Lopez and Anne 2015). ...

Simultaneous saccharification and fermentation of acid pretreated Shorea robusta for bioethanol production
  • Citing Article
  • April 2018

... 6. Medicinal properties of fungi of the genus Pleurotus Mushrooms of the genus Pleurotus are not only appreciated for their organoleptic and nutritional properties, but also for their medicinal properties (Juárez-Hernández et al., 2023). Medicinal properties are mainly attributed to primary and secondary metabolites (bioactive compounds) present in the fruiting bodies of the fungus (Sen et al., 2021), in the mycelium (Illuri et al., 2022) and in the culture medium derived from mycelial growth (Ogidi et al., 2020;Salmones, 2017). Among the main medicinal activities of secondary metabolites are the following: antibacterial, antiviral, antifungal, antitumor, anti-inflammatory, antiallergic and immunomodulatory activity (Torres-Martínez et al., 2022, Beltrán-Delgado et al., 2020. ...

Nutrients and Bioactive compounds of Pleurotus ostreatus mushroom

... The efficient utilization of agriculture crop residues and the fiber-rich co-products of agro-based industries as an economical and sustainable feed or biofuel stock has become a tion potential [14,20]. C. indica was selected as the fungus requires a low spawning rate [21] and substrate moisture level [22] and was thus expected to have greater rate and extent of substrate colonization and delignification. A. bisporus was selected due to its higher Mn peroxidases production potential [23], which plays a key role in lignin degradation and the early formation of mycelia [24]. ...

Review on production technique and nutritional status of Calocybe indica

... Owing to their unique properties, nano-biocatalysts have emerged as potential materials for enhancing biofuel generation. Typically employed as reactant specialists, chelates contribute greatly to electron transfer, s inhibitory combinations and promoting the activity of anaerobic groups [4][5][6]. ...

A Review on Role of Nanomaterials in Bioconversion of Sustainable Fuel Bioethanol

Waste and Biomass Valorization

... Biochemicals and biomolecules also have an important weight in the literature, obtained mainly by fermentation processes, but also by extraction processes, with supercritical fluid extraction standing out, followed by ultrasound-assisted extraction. Some of the products included in this category are lactic acid (obtained by the fermentation of mixed bakery waste [43], fruit waste [44], potato waste [45] and/or food waste [46]) ferulic acid (produced by an enzymatic extraction process using as raw material industrial residues as brewers spent grains [47]), xylooligosaccharides (mainly using agrowaste and considering enzymatic conversion or hydrothermal treatment for its production [48,49]), or enzymes such as alpha amylase, produced by the fermentation of rice by-products [50], or β-1,4-endoxylanase, obtained by the enzymatic hydrolysis of wheat straw [51]. ...

Valorization of rice milled by-products (rice husk and de-oiled rice bran) into α-amylase with its process optimization, partial purification and kinetic study
  • Citing Article
  • September 2022

Process Biochemistry

... In the food industry, the high protein content of V. volvacea, which rivals that of animal-based sources, makes it an excellent ingredient for developing plant-based protein products [144]. This is particularly relevant in the context of the growing demand for vegetarian and vegan diets, where finding complete and high-quality protein sources is essential [145]. Additionally, the comprehensive amino acid profile, including essential amino acids such as leucine, lysine, and valine, supports muscle maintenance and overall health, making V. volvacea an attractive ingredient for health-focused food products [146]. ...

Potential Application of Edible Mushrooms in Nutrition-Medical Sector and Baking Industries
  • Citing Chapter
  • April 2022