S. Weiner’s research while affiliated with Weizmann Institute of Science and other places

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Publications (117)


Intrastereomic proteins from the asteroid Asterias rubens: Separation, partial characterization, and interactions with growing calcite crystals
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November 2020

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5 Reads

Ph. Dubois

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S. Weiner

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L. Addadi

Figure 26.1 View of the cave.
Figure 26.5 Artefacts from areas E and F (Tabun D type): 1-2, elongated retouched points on narrow thick Laminar blanks; 3, retouched blade on narrow thick Laminar blank; 4, elongated retouched point on wide thin Levallois blank; 5, short triangular Levallois product; 6, narrow thick blade from the Laminar system.
Hayonim Cave
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January 2017

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1,396 Reads

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4 Citations

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Baruch Arensburg

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[...]

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Stev Weiner
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The effect of hydration on mechanical anisotropy, topography and fibril organization of the osteonal lamellae

January 2013

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162 Reads

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34 Citations

Journal of Biomechanics

The effect of hydration on the mechanical properties of osteonal bone, in directions parallel and perpendicular to the bone axis, was studied on three length scales: (i) the mineralized fibril level (∼100 nm), (ii) the lamellar level (∼6 µm); (iii) the osteon level (up to ∼30 µm).We used a number of techniques, namely atomic force microscopy (AFM), nanoindentation and microindentation. The mechanical properties (stiffness, modulus and/or hardness) have been studied under dry and wet conditions. On all three length scales the mechanical properties under dry conditions were found to be higher by 30–50% compared to wet conditions. Also the mechanical anisotropy, represented by the ratio between the properties in directions parallel and perpendicular to the osteon axis (Anisotropy Ratio, designated here by AnR), surprisingly decreased somewhat upon hydration. AFM imaging of osteonal lamellae revealed a disappearance of the distinctive lamellar structure under wet conditions. Altogether, these results suggest that a change in mineralized fibril orientation takes place upon hydration.



Structural Control Over the Formation of Calcium Carbonate Mineral Phases in Biomineralization

July 2011

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69 Reads

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8 Citations

Calcite is the most common of the CaCO3 mineral phases. It has a very simple structure (Fig. 1a), that can essentially be rationalized as a deformed NaCl structure1. Because of this relation to the halite structure, this was one of the first structures solved by Bragg2. Aragonite is thermodynamically slightly less stable than calcite at ambient temperatures and pressures. Its calcium ions in the (001) calcium layer are almost in exactly the same positions as those of calcite, but it differs from calcite in terms of the carbonate ion organization (Fig. 1b). Vaterite is the least stable of these three polymorphs. It differs significantly from the other two, in that the planes of the carbonate molecules are perpendicular to the calcium ion planes (Fig. 1c).


Table 1: Archaeological samples and their locations. Tableeau 1 : Échantillons archéologiques et leurs localisations. 
Atomic Disorder in Fossil Tooth and Bone Mineral: An FTIR Study Using the Grinding Curve Method

April 2011

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135 Reads

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39 Citations

ArchéoSciences / Journal of Archaeometry

Bone and tooth mineral generally undergo diagenetic changes. These changes in the carbonate hydroxyapatite structure and composition can affect the signals embedded in the mineral phase, such as migration behavior, age of the specimen and the reconstruction of past environments. Mineral preservation state can be assessed using infrared spectroscopy which provides information on crystal disorder at the atomic level and mineral composition. Here we present a new approach to evaluate carbonate hydroxyapatite atomic disorder using infrared spectroscopy and the standard KBr sample mounting method. We show that by repeated grinding of the sample and then plotting the infrared splitting factor against the width of the major phosphate absorption peak after each grinding, grinding curves with well defined trend lines can be obtained. The offsets between curves reflect differences in atomic disorder. We show that grinding curve offsets can be used to evaluate the state of preservation of bone, dentine and enamel mineral.


Differences between Bond Lengths in Biogenic and Geological Calcite

March 2010

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133 Reads

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81 Citations

Crystal Growth & Design

We used high-resolution neutron powder diffraction to accurately measure the atomic positions and bond lengths in biogenic and geological calcite. A special procedure for data analysis was developed in order to take into account the considerable amounts of magnesium present in all the investigated samples. As a result, in biogenic calcite we found some atomic bonds to have significantly different lengths as compared to those in geological calcite, after the contribution of magnesium is accounted for. The maximum effect (elongation up to 0.7%) was found for the C-O bonds. We also analyzed changes in frequencies and spectral widths of normal vibrations of carbonate groups in biogenic calcite (as compared to geological calcite) measured by Raman and Fourier transform IR techniques. Surprisingly, the frequency shifts after subtracting the magnesium contribution are close to zero. At the same time, substantial spectral broadening (up to 1.2%) in biogenic calcite as compared to geological samples was detected. Possible explanations for the experimental findings are discussed.


Ancient Mtdna Sequences And Radiocarbon Dating Of Human Bones From The Chalcolithic Caves Of Wadi El-Makkukh.

January 2010

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575 Reads

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14 Citations

Mediterranean Archaeology and Archaeometry

DNA from fossil human bones can provide valuable information for understanding intra- and inter-population relationships. Using the DNA preserved inside crystal aggregates from human fossil bones containing relatively large amounts of collagen, we demonstrate the presence of reproducible mtDNA control region sequences. Radiocarbon dates from each bone show that the burial caves were used for up to 600 years during the Chalcolithic period (5th-4th millennium BP). A comparison of the ancient DNA sequences with modern mtDNA databases indicates that all samples can most likely be assigned to the R haplogroup sub-clades, which are common in West-Eurasia. In four cases more precise and confident haplogroup identifications could be achieved (H, U3a and H6). The H haplogroup is present in three out of the four assigned ancient samples. This haplogroup is prevalent today in West - Eurasia. The results reported here tend to genetically link this Chalcolithic group of individuals to the current West Eurasian populations.


Figure 1-Grotte de Kébara : vue partielle de la coupe ouest montrant la superposition de nombreuses structures de combustion (niveaux noirs/blancs) dans les niveaux du Paléolithique moyen. Le pendage des couches est dû à un phénomène de soutirage karstique.
Figure 2-Hayonim Cave: infrared spectrometer at work in the field.
Figure 5-Hayonim Cave: global spatial distribution of the Middle Paleolithic lithic tools (above) and the bone remains from the same levels (300 to 540 cm) in the central zone of the cave (surface unit = 1 m 2 ). The greatest density of tools visible on the upper plan corresponds to a well marked concentration of material present in the base level of the stratigraphy, after Stiner 2005).
Figure 6-Grotte d'Hayonim : répartition des minéraux dominants dans les sédiments (en haut) et des densités de restes osseux (par poids, en grammes) dans le niveau 440/449 cm, couche E. La zone grise sur le plan du haut correspond à des sédiments riches en calcite (c) et en dahllite (d), constituant donc un milieu favorable à la conservation de l'os. Dans la zone laissée en blanc dominent au contraire les phosphates authigènes [montgoméryite (g), leucophosphite (l)] et les agrégats siliceux (s), éléments qui caractérisent un contexte défavorable à la conservation (d'après Stiner 2005). Les lignes continues entre ces zones représentent le « front de dissolution ». Leur cartographie précise a pu être réalisée grâce à une série d'analyses effectuées, dans ce but, sur le terrain ; les échantillons sont donc plus nombreux autour de ces limites (Nombre total d'échantillons traités = 84).
Figure 7-Kebara Cave: excavation in the central zone, unit X. Cartography of the dominant minerals inside and outside the three bone concentrations. The dotted line marks the limit between a zone in which dahllite dominates (a context thus favorable to bone preservation) and a zone in which secondary minerals (here globally called "other minerals") are present. It thus represents the "dissolution front" for this level (after Weiner et al. 1993).
Apport des analyses minéralogiques (en spectrométrie infra-rouge Transformation de Fourier) à l’interprétation des structures anthropiques : les concentrations osseuses dans les niveaux moustériens des grottes de Kébara et Hayonim (Israël)

January 2010

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358 Reads

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7 Citations

deS AmiS du muSée nAtionAl de PréhiStoire et de lA recherche Archéologique AVec le concourS du miniStÈre de lA culture et de lA communicAtion, du muSée nAtionAl de PréhiStoire et du centre nAtionAl de lA recherche ScientiFique PALEO -Supplément n o 3 Décembre 2010 PALEO reVue d'Archéologie PréhiStorique 3 édition : Actes du workshop n o 16 -XV e congrès international de l'UISPP Lisbonne, septembre 2006 mise en commun des approches en taphonomie Sharing Taphonomic Approaches



Citations (83)


... The SB-HP technocomplexes mostly correspond to Marine Isotope Stage 4 (an important cooling stage). In the same manner, the re-appearances of Aterian mostly correspond to a cooling trend (significant gene flows and so genetic enrichment), such as 110 ka ago at Dar-es-Soltane I Cave, c. 90 ka ago at Oued el Akarit, Tunisia, and 80-70 ka ago at Rhafas Cave, Morocco [121,122]. The same process seems valid among Neandertals. ...

Reference:

Birth of modern human neither in Africa nor gradual, but macroevolutionary in the Levant circa 150,000 years ago
The Rhafas Cave (Morocco): Chronology of the mousterian and aterian archaeological occupations and their implications for Quaternary geochronology based on luminescence (TL/OSL) age determinations
  • Citing Article
  • January 2007

... The archaeologic deposits in Hayonim, a relict phreatic cave (Fig. 1e) twelve km SSE of Manot Cave, yielded TL ages ranging from 230 to 140 ka for the lower part of its Middle Paleolithic sequence (Mercier et al., 2007;Bar-Yosef et al., 2017). Relict caves, such as the ones used by prehistoric hominins, have been attributed to speleogenesis from Neogene (Wakshal, 1979, p. 150) to early Pleistocene age (Picard, 1943, p. 143;Nir, 1989, p. 293). ...

Hayonim Cave

... Any substance or combination of substances that is implanted into or added to a biological system to support or replace the function of living tissue or organs is referred to as a biomaterial [1]. Due to their chemical similarity to the mineral that builds up mammalian bones and teeth, calcium phosphates are primarily employed as bone substitute materials [2][3][4]. So, they are both nontoxic, biocompatible, and the body doesn't see them as foreign materials. The fact that they both integrate into live tissue through the same processes employed to repair healthy bones is most essential. ...

On biomineralization
  • Citing Article
  • January 1989

... By mass, its composition includes approximately 60% hard, mineral phase (primarily semi-crystalline, carbonated hydroxyapatite), 30% soft, organic phase involving type I collagen fibrils and noncollagenous proteins, and 10% water. 1 Consequently, the biomechanical characteristics of bone are influenced by the quality and spatial organization of these components. The contributions of mineral and collagen phases to bone mechanical competence have been well documented. ...

Bone structure- from Angstroms to microns
  • Citing Article
  • January 1991

... Shortterm occupations are characterized by few remains, small areas, significant carnivore activity, and lithic tools configured outside the camp (Costamagno et al. 2011;Niven et al. 2012;Picin and Cascalheira 2020;Vallerdú et al. 2005;Villaverde et al. 2017). At the archaeological level, it has been possible to identify sites used as long-term camps (Bar-Yosef et al., 1992;Blasco et al. 2016;Daujeard, 2008;Morin 2012;Rendu 2010;Romandini et al. 2014), and short-term (Castel et al. 2017;Conard et al. 2012;Delagnes and Rendu 2011;Jauberte et al. 2008;Marín-Arroyo 2013;Rendu and Armand 2009;Rosell et al., 2012b;Valensi et al. 2012) and special purpose sites such as intermediate slaughter or butchering places (Costamagno et al. 2006;Rendu et al. 2012). In the Iberian Peninsula, we find numerous sites in which Neanderthals are the main accumulating agent and which are interpreted as long-term (Gabucio et al. 2014;Luret et al. 2020;Marín et al. 2019;Romero et al. 2019;Rosell et al. 2012a, b;Sañudo et al. 2016;Villaverde et al. 2017) and short-term residential sites, especially hunting camps or hunting stands (Baldeón, 1993;Bargalló et al. 2020;Martínez-Moreno et al. 2004;Moclán et al. 2021;Pérez et al. 2017aPérez et al. , b, 2020Real Margalef et al. 2019;Rios-Garaizar et al. 2015;Salazar-García et al. 2013;Sánchez-Romero et al. 2020;Sanchis et al. 2019;Utrilla et al. 2010;Uzquiano et al. 2012). ...

Comment: The excavations in Kebara Cave, Mt. Carmel [and comments and replies]

... A minimum of 200 phytoliths was examined at 200Â and 400Â using a Nikon Eclipse 50i optical microscope. Morphological identification was based on modern plant reference collections and standard literature (Albert and Weiner 2001;Albert et al. 2008, Albert, Ruiz, andSans 2016;Brown 1984;Mulholland and Rapp 1992;Twiss, Suess, and Smith 1969;Piperno 2006;Portillo et al. 2014;Rosen 1992;Tsartsidou et al. 2007;Twiss 1992). The International Code for Phytolith Nomenclature (ICPN 2.0) was also followed where appropriate (Neumann et al. 2019). ...

Study of phytoliths in prehistoric ash layers using a quantitative approach

... Subsequently, bone and tooth samples with higher collagen concentrations are fluorescing stronger (Bachman and Ellis 1965;Mazel 2017;Miranda et al. 2017). Subsequently, the mineralogical preservation of samples was corroborated using FTIR (Asscher et al. 2011). ...

Atomic Disorder in Fossil Tooth and Bone Mineral: An FTIR Study Using the Grinding Curve Method

ArchéoSciences / Journal of Archaeometry

... Hayonim Cave is located in western Galilee, Israel. Two series of excavations were carried out on site (for history of excavations, see Bar-Yosef et al. 2005). The Natufian was the last prehistoric occupation in the cave, capping Mousterian, Aurignacian and Kebaran habitations. ...

Archaeological background to Hayonim Cave and Meged Rockshelter

... Integral matrix proteins are intimately associated with the mineral phase of the spicule, thus making them likely candidates to play a role in stabilizing ACC. To address this possibility, Raz et al. 27 inquired whether the spicule integral matrix proteins could stabilize ACC in Vitro. They found that spicule matrix proteins isolated from earlier stage spicules that have high levels of ACC (from late gastrula/prism stage embryos) will stabilize amorphous calcium carbonate in Vitro in the presence of Mg 2+ , while spicule matrix proteins isolated from older spicules that have little ACC (from pluteus stage) will not. ...

Adv. Funct. Mater.
  • Citing Article
  • January 2003

Advanced Functional Materials

... [11][12][13][14] Many naturally occurring crystals have shapes which do not correlate with their crystallographic symmetry.B iomineralization can afford materials with superior properties, complex morphologies and hierarchical order. [15][16][17][18] Most of these natural materials are based on single crystals of calcium carbonate.T he existence of specific and complex morphologies is ad irect result of the evolutionary approach of nature and needed to fulfill various tasks.For example,coccoliths are composed of calcium carbonate which is known to be brittle. However,the interlocking architecture significantly enhances their damage tolerance. ...

On the Concept of a Single Crystal in Biomineralization
  • Citing Chapter
  • January 1999