S.-L. Yang’s research while affiliated with Jiangxi University of Traditional Chinese Medicine and other places

Objective To investigate the chemical constituents from Disporum cantoniense. Methods Compounds were isolated and purified by macroporus resin, ods, Sephadex LH-20, MCI-gel CHP20 resin column chromatography and preparative HPLC. The structures were identified by spectral analysis. Results Twenty compounds were isolated and identified as 2-hydroxybenzyl alcohol (1), p-hydroxybenzaldehyde (2), 4-hydroxyacetophenone (3), 3,5-dimethoxy-4-hydroxy-benzaldehyde (4), 4-(4-hydroxyphenyl)-2- butanone (5), neoliquiritin (6), 2,3,5,4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside (7), (E)-1-(4’-hydroxyphenyl)-but-1-en-3-one (8), isoquercitrin (9), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxy-phenyl)-1-propanone (10), icariol A2 (11), ecdysterone (12), glansreginic acid (13), hesperidin (14), ononin (15), quercetin (16), isorhamnetin-3-O-β-D-glucoside (17), (E)-4-(4-hydroxy-3-methoxyphenyl) but-3-en-2-one (18), (-)-secoisolariciresinol (19), and luteolin (20). Conclusion Compounds 1, 3-8, 10-15, and 17-19 are isolated from the genus of Disporum for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the chemical constituents from the whole plants of Gynura procumbens. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and ODS. Their structures were determined by physicochemical properties and spectroscopic analysis. Results Twenty-seven compounds were identified as dibutyl phthalate (1), ursolic acid (2), kaempferol 3-O-β-D-glucopyranoside (3), 5-hydroxymaltol (4), 4-hydroxylbenzoic acid (5), 4-aminocinnamic acid (6), (E)-2-hexenyl β-D-glucoside (7), 1-(3-indolyl)-2,3-dihydroxy-propan-1-one (8), kaempferol-7-O-β-D-glucoside (9), quercetin-3-O-β-D- glucopyranoside (10), 3,4,5-tri-caffeoylquinic acid methyl ester (11), rutin (12), hesperidin (13), 3,4-dihydroxyphenylacetic acid methyl ester (14), euscophic acid (15), tormentic acid (16), 2-methoxy-4-(2-propenyl)-phenyl-O-β-D-glucopyranoside (17), negletein (18), 4,5-dihydroxy-3-methoxybenzoic acid (19), caesalpiniaphenol D (20), gentisic acid (21), 3,4-dihydroxybenzaldehyde (22), isohematinic acid (23), icariside B 1 (24), dendranthemoside B (25), 4-methoxycinnamic acid (26), and baicalin (27). Conclusion Compounds 1, 2, 10, and 12 are obtained from the plants of G. procumbens for the first time, and compounds 4, 6-9, 11, 13-16, and 17-27 are obtained from Gynura genus for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the chemical constituents from the leaf of Camellia sinensis. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, ODS column chromatography, high pressure flash chromatography and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Nine compounds were separated from C. sinensis, among them, four compounds were identified as triterpenoids and named as 21β-hydroxyl pomolic acid (1), pomonic acid (2), pomolic acid (3), and ursolic acid (4); The other five compounds were idetified as triterpenoid saponins and named as 3-O-α-L-arabinopyranosyl pomolic acid (5), 3β-[(α-L-arabinopyranosyl)oxy]-urs-12,19 (20)-dien-28-oic acid (6), oleanolic acid-3-O-α-L-arabinopyranoside (7), 3β-[(α-L-arabinopyranosyl) oxy]-urs-12,18-dien-28-oic acid (8), and 20-epi-urs-12,18-dien-28-oic acid 3β-O-α-L-arabinopyranoside (9). Conclusion Compound 1 is a new compound, compounds 2, 3, 5-9 are obtained from this genus for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To study the saponins from Reineckia carnea of Guizhou Miao medicine. Methods: The chemical constituents from 95% EtOH extract were isolated and purified by repeated silica gel column chromatography, macroporous resin, ODS C18, Sephadex LH-20 gel column chromatography, and Pre-HPLC, and their structures were identified on the basis of physical and chemical properties, HR-ESI-MS and NMR spectral analysis. Results: Ten compounds were isolated and identified as (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol1,16-di (β-D-glucopyranosede) (1), (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol-1-[O-α-L-rhamno-pyranosyl-(1→2)-β-D-glucopyranoside]-16-(β-D-glucopyranoside) (2), (20S,22R)-spirost-25(27)-en-1β,3β,4β,5β-tetraol-5-O-β-D-glucopyranoside (3), kitigenin 5-O-β-D-glucopyrannoside (4), aspidistrin A (5), (17,20-S-trans)-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-(1→2)-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside (6), diosgenin-3-O-β-D-glucopyranoside (7), ophiopogonin T (8), prosaikogenin D (9), and saikosaponin b2 (10). Conclusion: Compounds 3, 5, 8, 9, and 10 are isolated from this plant for the first time, which provided some reference for further elucidation and development of saponins from R. carnea. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To study the chemical constituents from the Sabia parviflora. Methods Various column chromatographic techniques were used to separate and purify the chemical constituents which structures were elucidated by spectral analysis. Results Fifteen compounds were isolated and identified as bis (2-ethylhexyl) benzene-1,2-dicarboxylate (1), dibutyl phthalate (2), darutigenol (3), sucrose (4), 3,5-dimethoxy-4-hydroxybenzaldehyde (5), vanillin (6), cleomiscosin C (7), grossamide (8), (-)-lyoniresino (9), ervatamisin (10), (+)-syringaresinol (11), seslignanoccidentaliol A (12), (+)-syringaresinol-4-O-β-D-glucopyranoside (13), (-)-simulanol (14), and (-)-7R,8S-dehydrodiconiferyl alcohol (15). Conclusion All the compounds are isolated from the genus of Sabia for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the steroidal chemical constituents from the whole plants of Physalis minima. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure ODS column chromatography and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical properties and spectral analysis. Results Seven compounds were separated from the whole plant of P. minima, among them, two compounds were identified as physalins and named as 16,24-cyclo-13,14-secoergost-2-ene-18,26- dioicacid-14:17,14:27-diepoxy-5α,6β,13α,20α,22β,25β-hexahydroxy-1,15-dioxo-γ-lactone-δ-lactone (1) and physalin D (2); The other five compounds were identified as withanlides and named as physagulin L (3), physagulin M (4), physaminimin F (5), physagulin K (6), and withagulatin A (7). Conclusion Compound 1 is a new compound named 25-hydroxy-physalin D, at the same time, compounds 3, 4, and 7 were obtained from this plant for the first time. © 2018, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective To investigate the chemical constituents from the seeds of Cuminum cyminum. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, high pressure flash chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Fifteen compounds were identified as astragaline (1), 6-β-hydroxy glycyrrhetinic acid (2), ursolic acid (3), quercetin (4), protocatechuic acid (5), (6R,7E,9R)-9-hydroxy-4,7- megastigma-dien-3-one (6), 4,5-dicaffeoylquinic acid methyl ester (7), 3,5-dicaffeoylquinic acid methyl ester (8), rosin (9), kaempferol 3-O-β-D-glucopyranosyl (1→2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (10), cuminacid (11), cuminoside A (12), luteolin (13), apigenin-7-O-β-D-(6″-O-acetyl)-glucopyranoside (14), and 5,7,4’-trihydroxyflavanone (15). Conclusion Compounds 2, 3, 6-10, and 14 are obtained from this genus for the first time. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To establish and validate the design space of spray drying process of Fengke Granule using the concept of quality by design (QbD). Methods: Using Fengke Granule formula to extract the concentrated solution as model drug, the risk assessment and Plackeet-Burmann design on influence factors of screening, to determine the liquid inlet velocity and pressure of atomization is the critical process parameters. Then, the central composite design was used to optimize the key parameters and establish the process design space. Finally, four experimental points were selected to test the predictive power of the model. Results: Analysis of variance results showed that regression model P values were less than 0.01, which showed that the model had a good prediction, and the optimal range of key parameters of inlet velocity was determined and pressure of atomization were 11%-14% and 41.3-45.0 mmHg, the process parameters in this range could meet the target requirements. Conclusion: The design space of spray drying process is established based on QbD, which could improve the flexibility and stability of spray drying process, and provide reference for the pilot study in the future. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, high pressure flash chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results: Fourteen compounds were identified as syringin (1), 3, 4-dihydroxyphenyl ethanol (2), oleuropein (3), salidroside (4), oleoside 11-methyl ester (5), (8E)-nuezhenide (6), (8Z)-ligstroside (7), oleoacteoside (8), oleoside dimethyl ester (9), olivil-4'-O-β-D-glucoside (10), (+)-cyclo-olivil-6-O-β-D-glucoside (11), (+)-cyclo-olivil-4'-O-β-D-glucoside (12), ligstroside (13), and wilfordiol B (14). Conclusion: Compounds 2, 4, and 12-14 are obtained from this genus for the first time, and compounds 1, 3, and 5-11 are obtained from this plant for the first time. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.

Objective: To study the chemical constituents from Disporum cantoniense. Methods: Various column chromatographic techniques were used to separate and purify the chemical constituents and their structures were elucidated by spectral ananlysis. Results: Sixteen compounds were isolated and identified as 3-maleimide-5-oxime (1), 4-methylene-5-oxopyrrolidine-2- carboxylic acid (2), thymine (3), adenosine (4), 5'-deoxy-5'-methylamino-adenosine (5), ethyl-α-L-rhamnose (6), bergenin (7), 4-hydroxy- 2-methoxyphenyl-6-deoxy-α-L-talopyranoside (8), (-)-epicatechin (9), (6R,9R)-roseoside (10), 3-(4-hydroxy-3,5-dimethoxyphenyl) propane-1,2-diol (11), 3-hydroxy-5-(p-hydroxyphenyl) pentanoic acid (12), 1-ribityl-2,3-diketo-1,2,3,4-tetrahydro-6,7-dimethyl-quinoxaline (13), (6S,9R)-vomifoliol (14), 1-(3,4-dihydroxyphenyl)-2-hydroxye-thanone (15), and 1,2-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)- ethane (16). Conclusion: Compounds 1, 2, 5-9, and 12-16 are isolated from genus Disporum Salisb. for the first time, and compounds 1, 2, 5-10, and 12-16 are isolated from this plant for the first time. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.