S. Christopher Marble’s research while affiliated with University of Florida and other places

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Publications (9)


Tradescantia fluminensis flower (left) and example of spreading growth habit (right).
Gibasis pellucida in flower (left) and example of spreading growth habit (right).
Mean visual ratings of Tahitian bridal veil (Gibasis pellucida) (A) and small-leaf spiderwort (Tradescantia fluminensis) (B) control following treatment with selected herbicides (triclopyr acid, triclopyr amine + 2,4-D amine, triclopyr amine, and glufosinate) at 2, 4, and 8 weeks after treatment (WAT). Means and standard error bars are shown and are pooled over two experimental runs.
Mean Tahitian bridal veil (Gibasis pellucida) shoot dry weight (A) and regrowth (B) (presented in grams) following treatment with selected herbicides including triclopyr acid, triclopyr amine + 2,4-D amine, triclopyr amine, and glufosinate. Mean shoot dry weight and regrowth for small-leaf spiderwort (Tradescantia fluminensis) are shown in graphs (C,D). Mean shoot dry weight for both species (A,C) was collected at 8 weeks after treatment, while shoot regrowth (B,D) was collected at 4 weeks following the initial harvest (12 weeks after the initial treatment).
Selected herbicides evaluated for postemergence control of Gibasis pellucida and Tradescantia fluminensis in greenhouse experiments in Florida.
Response of Tahitian Bridal Veil (Gibasis pellucida) and Small-Leaf Spiderwort (Tradescantia fluminensis) to Postemergence Herbicides under Greenhouse Conditions
  • Article
  • Full-text available

May 2024

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12 Reads

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Stephen Christopher Marble

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Tahitian bridal veil (Gibasis pellucida) and small-leaf spiderwort (Tradescantia fluminensis) are both invasive species in natural areas throughout Florida. However, very little is known regarding herbicide control. To provide land managers with herbicidal control options for both species, postemergence herbicides were evaluated for efficacy in a greenhouse to identify herbicide options that control both species under similar settings. Four herbicides, including triclopyr acid, triclopyr amine + 2,4-D amine, triclopyr amine, and glufosinate were applied at standard label rates and compared to a non-treated control group for efficacy. Visual control ratings were taken at 2, 4, and 8 weeks after treatment (WAT), and shoot dry weights (WAT 8) and regrowth dry weights (WAT 12) were determined. Triclopyr (acid and amine) generally provided the most consistent control of both species as evidenced by the visual control ratings and shoot dry weight data which showed reductions of 76% to 89% in shoot biomass at trial conclusion. Triclopyr + 2,4-D reduced shoot dry weights by 52% to 54% and was the least effective when considering the control of both species.

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FIGURE 1 Examples of commonly used mulch material in container plant production consisting of (A) Crape myrtle (Lagerstroemia indica L.) mulched with pine bark, (B) Crape myrtle mulched with rice hulls, (C) Podocarpus (Podocarpus macrophyllum var. maki) mulched with shredded hardwood and (D) Podocarpus mulched with sawdust.
FIGURE 2
FIGURE 3
Examples of effective mulch materials and required depths previously evaluated for use in container plant production.
Mulching as a weed management tool in container plant production - review

December 2023

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216 Reads

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5 Citations

Frontiers in Agronomy

Weed management in container plant production is a serious problem and remains one of the most expensive and time-consuming aspects of the industry. Weeds cause severe reductions in crop growth due to the limited growing environment characteristic of container plant production. The container nursery industry relies heavily on a limited number of preemergence herbicide options. The use of herbicides as the primary means to manage weeds has resulted in some negative consequences such as high chemical costs, leaching, runoff, and concerns with recycling irrigation water. Additionally, nursery growers are shifting their focus toward different nonchemical weed management strategies because many ornamental plants are very sensitive to preemergence herbicides. One such method is using organic mulch to control weeds in container plant production. Mulching is the foundation of a nonchemical weed management protocol and acts as the first line of defense against weeds. Organic mulches used in container plant production include rice hulls, pine bark, wood chips, wood shavings, coconut coir, nut (peanut, pecan) shells, oyster shells, cacao bean hulls, pelletized newspaper, recycled newspaper, pine straw, and other materials; material selection often depends on the availability of the product. The objective of this manuscript is to provide a comprehensive review of existing research on the utilization of various mulch materials as a weed management tool in container plant production. Additionally, it aims to highlight any critical knowledge gaps and provide suggestions for possible future research.


Fig. 1. Average monthly temperature and cumulative rainfall over two experimental runs [Mar to Nov 2020 (Florida Automated Weather Network 2022)]. The first experimental run was initiated on 11 Mar 2020, and the second run on 12 May 2020, with both experiments being harvested at 24 weeks after planting; ( F -32) 4 1.8 5 C, 1 inch 5 2.54 cm.
Fig. 2. Established seedlings of spotted spurge at 4 weeks after planting. Substrate consisted of 1/8 to 1/4-inch pine bark (PB) at 1 inch depth (1/8-1/4:S:1), 1/8 to 1/-inch PB at 2 inches depth (1/8-1/4:S:2), 1/4 to 1/2-inch PB at 1 inch depth (1/4-1/2:S:1), 1/4 to 1/2-inch PB at 2 inches depth (1/4-1/2:S:2), 3/8 to 3/4-inch PB at 1-inch depth (3/8-3/ 4:S:1), 3/8 to 3/4-inch PB at 2 inches depth (3/8-3/4:S:2), #1.2-inch PB throughout (#1/2:TO); 1 inch 5 2.54 cm.
Fig. 3. Liverwort coverage at 16 weeks after planting. Substrate consisted of 1/8 to 1/4-pine bark (PB) at 1 inch depth (1/8-1/4:S:1), 1/8 to 1/4-inch PB at 2 inches depth (1/8-1/4:S:2), 1/4 to 1/2-inch PB at 1 inch depth (1/4-1/2:S:1), 1/4 to 1/2-inch PB at 2-inch depth (1/4-1/2:S:2), 3/8 to 3/4-inch PB at 1 inch depth (3/8-3/4:S:1), 3/8 to 3/4-inch PB at 2 inches depth (3/8-3/4:S:2), #1/2-inch PB throughout (#1/2:TO); 1 inch 5 2.54 cm.
Effect of Substrate Stratification Without Fine Pine Bark Particles on Growth of Common Nursery Weed Species and Container-grown Ornamental Species

December 2022

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109 Reads

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8 Citations

HortTechnology

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S. Christopher Marble

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James E. Altland

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[...]

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Pratap Devkota

Substrate stratification is a new research area in which multiple substrates, or the same substrate with differing physical properties, are layered within a container to accomplish a production goal, such as decreasing water use, nutrient leaching, or potentially reducing weed growth. Previous research using stratification with pine ( Pinus sp.) bark screened to ≤1/2 or 3/4 inch reduced the growth of bittercress ( Cardamine flexuosa ) by 80% to 97%, whereas liverwort ( Marchantia polymorpha ) coverage was reduced by 95% to 99%. The objective of this study was to evaluate substrate stratification with pine bark screened to remove all fine particles as the top strata of the substrate and determine its effect on common nursery weeds and ornamental plants. Stratified treatments consisted of pine bark screened to either 1/8 to 1/4 inch, 1/4 to 1/2 inch, or 3/8 to 3/4 inch, applied at depths of either 1 or 2 inches on top of a standard ≤1/2-inch pine bark substrate. An industry-standard treatment was also included in which the substrate was not stratified but consisted of only ≤1/2-inch pine bark throughout the container. A controlled-release fertilizer was incorporated at the bottom strata in all stratified treatments (no fertilizer in the top 1 or 2 inches of the container media), whereas the industry standard treatment had fertilizer incorporated throughout. Compared with the nonstratified industry standard, substrate stratification decreased spotted spurge ( Euphorbia maculata ) counts by 30% to 84% and bittercress counts by 57% to 94% after seeding containers. The shoot dry weight of spotted spurge was reduced by 14% to 55%, and bittercress shoot dry weight was reduced by 71% to 93% in stratified treatments. Liverwort coverage was reduced by nearly 100% in all the stratified substrate treatments. Compared with the industry standard substrate, stratified treatments reduced shoot dry weight of ligustrum ( Ligustrum japonicum ) by up to 20%, but no differences were observed in growth index, nor were any growth differences observed in blue plumbago ( Plumbago auriculata ).


Particle size distribution of pine bark, rice hulls, and peatmoss mixed soil evaluated for Pennsylvania bittercress growth.
Improving Pennsylvania Bittercress Weed Control Efficacy with Mulch and Herbicide in Containers

December 2022

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59 Reads

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1 Citation

HortTechnology

Pennsylvania bittercress ( Cardamine pensylvanica ) and other bittercress ( Cardamine ) species are among the most common and difficult-to-control weed species in container nurseries, and they have been vouched in most counties in Florida. Preemergence herbicides can provide control, but concerns over potential resistance development, environmental issues, and crop injury problems associated with herbicide use create the need for alternative weed control methods to be explored. Previous studies have shown the potential of mulch materials for controlling weeds in nurseries, but their use along with preemergence herbicides has not been extensively investigated. To compare the effects of different mulch materials and herbicides on Pennsylvania bittercress control, a full factorial designed greenhouse study was conducted. Three mulch treatments including no mulch, pine ( Pinus sp.) bark, and rice ( Oryza sativa ) hulls were evaluated with three herbicide treatments, including water (i.e., no herbicide), isoxaben, and prodiamine applied at label rates. Twenty-five seeds of Pennsylvania bittercress were sown on the surface of each container and emergence (percent), coverage (square centimeters), seedhead number, and biomass (grams) were measured. The results showed that Pennsylvania bittercress in containers mulched with rice hulls had the lowest emergence throughout the experiment. For coverage, seedhead, and biomass parameters, Pennsylvania bittercress seeded in rice hulls treatments had significantly lower coverage, fewer seedheads, and lower biomass compared with those in nonmulched or pine bark treatments, regardless of herbicide treatment. With isoxaben and the water check, nonmulched treatments had the highest coverage/seedhead/biomass, whereas with prodiamine, Pennsylvania bittercress in pine bark mulched containers had the highest coverage/seedhead/biomass. In conclusion, applying rice hulls alone can provide better Pennsylvania bittercress control compared with isoxaben or prodiamine applied alone.


Representive allelochemicals from the classified 14 categories based on their chemical similarities.
Selected seven allelochemicals from the 14 categories, their sources, and the allelochemicals present in them.
Allelopathy and its application as a weed management tool: A review

November 2022

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1,908 Reads

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99 Citations

Weeds are a serious threat to crop production as they interfere with the crop growth and development and result in significant crop losses. Weeds actually cause yield loss higher than any other pest in crop production. As a result, synthetic herbicides have been widely used for weed management. Heavy usage of synthetic herbicides, however, has resulted in public concerns over the impact of herbicides on human health and the environment. Due to various environmental and health issues associated with synthetic herbicides, researchers have been exploring alternative environmentally friendly means of controlling weed. Among them, incorporating allelopathy as a tool in an integrated weed management plan could meaningfully bring down herbicide application. Allelopathy is a biological phenomenon of chemical interaction between plants, and this phenomenon has great potential to be used as an effective and environmentally friendly tool for weed management in field crops. In field crops, allelopathy can be applied through intercropping, crop rotation, cover crops, mulching and allelopathic water extracts to manage weeds. Accumulating evidence indicates that some plant species possess potent allelochemicals that have great potential to be the ecofriendly natural herbicides. This review is intended to provide an overview of several allelopathic species that release some form of the potent allelochemical with the potential of being used in conventional or organic agriculture. Further, the review also highlights potential ways allelopathy could be utilized in conventional or organic agriculture and identify future research needs and prospects. It is anticipated that the phenomenon of allelopathy will be further explored as a weed management tool, and it can be a part of a sustainable, ecological, and integrated weed management system.


Herbicide selection for controlling Tahitian bridal veil ( Gibasis pellucida )

October 2022

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41 Reads

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2 Citations

Invasive Plant Science and Management

Tahitian bridal veil [ Gibasis pellucida (M. Martens & Haleotti) D.R. Hunt], a Central and South America native plant that is often confused with another more well-known invasive plant, small leaf spiderwort ( Tradescantia fluminensis Vell.), has become invasive in natural areas throughout Florida. However, very little is known regarding herbicide control or other methods. To begin the process of developing herbicide recommendations for land managers who are working to control G. pellucida , multiple postemergence herbicides were screened for efficacy in a shaded greenhouse to determine active ingredients and/or combinations that warrant further investigation under field conditions. Nine different herbicides or combinations including glyphosate, triclopyr acid, 2,4-D+triclopyr, aminopyralid, 2,4-D, triclopyr amine, metsulfuron-methyl, fluroxypyr, and glufosinate were applied at standard label rates and compared with a non-treated control group. Visual control ratings were taken at 2, 4, and 8 weeks after treatment (WAT) and shoot dry weights were determined at trial conclusion (8 WAT). Data showed glufosinate and triclopyr (acid and amine) provided the highest level of control as evidenced by control ratings (100% or complete control) and shoot fresh weight reduction, followed by 2,4-D+triclopyr (∼70%) and fluroxypyr (∼50% control). 2,4-D, and metsulfuron-methyl provided the lowest level of control and were similar to non-treated plants on most evaluation dates.


Practice in Nursery Weed Control—Review and Meta-Analysis

February 2022

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353 Reads

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8 Citations

Weeds, as one of the biggest challenges in the nursery industry, have been controlled by various methods, such as chemical and non-chemical practices. Although these practices have been widely established and tested to control weeds, there is no systematic or meta-analysis review to provide quantitative weed control efficacy information of these practices. To provide a systematic understanding of weed control practices in nursery production, a visualization research trend, a systematic review, and a meta-analysis were conducted. A total of 267 relevant studies were included for the research trend and 83 were included in the meta-analysis. The results in this study showed that interests in nursery weed control have switched dramatically in the past 2–3 decades (1995–2021) from chemical dominant weed control to chemical coexistent with non-chemical techniques. Developing new management tactics and implementing diverse combinations of integrated weed management present the future trend for weed control. The systematic review results showed that chemical methods had the highest weed control efficacy, while non-chemical had the lowest on average, nonetheless, all three weed control practices (chemical, non-chemical, and combined) reduced the weed biomass and density significantly compared with when no strategy was employed. Weed control challenges could be the catalyst for the development of new non-chemical and integrated weed control techniques.


Fig. 1. Average monthly temperature and cumulative rainfall during both experimental runs (Nov. 2019 to July 2020). Experiment run 1 was initiated on 20 Nov. 2019, and experimental run 2 was initiated on 2 Feb. 2020, with both experiments being harvested at 24 weeks after planting. 1 inch = 2.54 cm, ( F 2 32) 4 1.8 = C.
Fig. 2. Liverwort coverage at 16 weeks after planting. 0.38:TO = 0.38-inch pine bark (PB) incorporated with controlledrelease fertilizer (CRF) throughout the container; 0.5:TO = 0.5-inch PB incorporated with CRF throughout the container; 0.75:TO = 0.75-inch PB incorporated with CRF throughout the container; 0.75:S:2:N/F = 0.75-inch bark with fines removed, applied at a depth of 2 inches with 0.38-inch bark as the bottom substrate with CRF incorporated; 0.5:S:2 = 0.5-inch PB as top substrate applied at a depth of 2 inches with 0.38-inch bark as the bottom substrate with CRF incorporated; 0.5:S:3 = 0.5-inch PB as top substrate applied at a depth of 3 inches with 0.38-inch bark as the bottom substrate with CRF incorporated; 0.75:S:2 = 0.75-inch PB as top substrate applied at a depth of 2 inches with 0.38-inch bark as the bottom substrate with CRF incorporated, 0.75:S:3 = 0.75-inch PB as top substrate applied at a depth of 3 inches with 0.38-inch bark as the bottom substrate with CRF incorporated; 1 inch = 2.54 cm.
Fig. 3. Example of ligustrum root growth in a stratified substrate. The plant on the left was grown in a substrate constructed with 0.75-inch pine bark at a depth of 3 inches used as the top substrate and 0.38-inch pine bark as the bottom substrate with fertilizer only incorporated in the bottom portion (0.75:S:3). The plant on the right was grown using a 0.38-inch pine bark throughout the substrate profile with a controlled-release fertilizer incorporated throughout the container at the same rate (0.38:TO). 1 inch = 2.54 cm.
Effect of Substrate Stratification on Growth of Common Nursery Weed Species and Container-grown Ornamental Species

January 2022

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294 Reads

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8 Citations

HortTechnology

Substrate stratification is a method of filling nursery containers with “layers” of different substrates, or different textures of the same substrate. Recently, it has been proposed as a means to improve drainage, substrate moisture dynamics, and optimize nutrient use efficiency. Substrates layered with larger particle bark as the top portion and smaller particle bark as the bottom portion of the container profile would theoretically result in a substrate that dries quickly on the surface, thereby reducing weed germination, but that would also retain adequate moisture for crop growth. The objective of this study was to evaluate the effect of stratified substrates on the growth of common nursery weeds and ornamental crops. This study evaluated the use of coarser bark (<0.5 or 0.75 inches) as the top substrate and finer bark (<0.38 inches) as the bottom substrate with the goal of reducing the water-holding capacity in the top 2 to 3 inches of the substrate to reduce weed germination and growth. Results showed that substrate stratification with more coarse bark on the top decreased the growth of bittercress ( Cardamine flexuosa ) by 80% to 97%, whereas liverwort ( Marchantia polymorpha ) coverage was reduced by 95% to 99%. Substrate stratification initially reduced the growth of ligustrum ( Ligustrum japonicum ) and blue plumbago ( Plumbago auriculata ), but there was no difference in the shoot or root dry weights of either species in comparison with those of nonstratified industry standard substrates at the end of 24 weeks. The data suggest substrate stratification could be used as an effective weed management strategy for container nursery production.


Efficacy of Postemergence Herbicides for Control of Small-Leaf Spiderwort (Tradescantia fluminensis) in Florida

April 2021

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20 Reads

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2 Citations

Natural Areas Journal

Tradescantia fluminensis (small-leaf spiderwort; SLSW) is a fast-growing herbaceous groundcover and one of the most problematic invasive plants in Florida. The objective of this research was to determine the efficacy of selected postemergence herbicides for SLSW control in greenhouse and field experiments in Florida. An additional objective was to determine if pre-cutting plants would increase herbicidal efficacy. In greenhouse experiments, cutting mature SLSW plants increased the control of most herbicides evaluated. Overall, triclopyr ester provided the highest level of control along with triclopyr amine, triclopyr choline, and glufosinate, all of which were similar. Few differences were observed between 2,4-D, aminopyralid, metsulfuron-methyl, sulfentrazone, and glyphosate and all provided 66% control as evidenced by shoot weight reduction. In field experiments, pre-cutting SLSW had no effect on herbicide efficacy. Triclopyr was again generally the most efficacious treatment, outperforming glyphosate, aminopyralid, glufosinate, and fluroxypyr on most evaluation dates, especially as trials progressed past 5 mo. Overall, data suggest that triclopyr would be the most effective option for SLSW management. However, as efficacy was noted with fluroxypyr, glyphosate, and glufosinate depending upon location, other options exist. Further research is needed to determine reapplication intervals and effects on nontarget native plants in order to develop comprehensive management plans.

Citations (8)


... Mulching is a water-saving technique used in arid regions to conserve soil moisture and reduce evaporation [129]. It involves covering the soil with materials like plastic or wheat straw to minimize moisture loss [130] and control weeds [131]. This method is particularly effective in vegetable and fruit farming, reducing the need for chemical herbicides [132] while retaining soil moisture for longer periods (Figure 16). ...

Reference:

Sustainable Soil and Water Management in Arid Climates in the Mediterranean Climate Zone
Mulching as a weed management tool in container plant production - review

Frontiers in Agronomy

... T. fluminensis has been the subject of numerous herbicide evaluations as it is a problematic species in Florida and other countries such as New Zealand and Brazil. Overall, triclopyr (multiple formulations) has been the most consistently effective option across many of the different studies [4,[11][12][13][14], yet triclopyr can damage many native broadleaf plant species [15], leading researchers to search for other viable non-chemical alternatives. Artificial shading (reducing ambient light by 80-90%) has been shown to reduce T. fluminensis cover by over 60% and was less injurious to native tree seedlings compared with the use of herbicides but was not feasible in large-scale infestations [15]. ...

Efficacy of Postemergence Herbicides for Control of Small-Leaf Spiderwort (Tradescantia fluminensis) in Florida
  • Citing Article
  • April 2021

Natural Areas Journal

... The following results were obtained for coarse (≥ 2 mm), medium (0.5-2 mm), and fine particles (≤ 0.5 mm in weight) (Khamare et al., 2022). Coarse particles: substrate 1 (17.3 %), substrate 2 (14.6 %), substrate 3 (14.8 ...

Effect of Substrate Stratification Without Fine Pine Bark Particles on Growth of Common Nursery Weed Species and Container-grown Ornamental Species

HortTechnology

... Literatürde yabancı ot yoğunluğunda toprak özelliklerinin ve mikroiklim koşullarının da etkili olduğu ifade edilmektedir (Gastonvd, 2001;James vd, 2006 (Üremiş & Kuru, 2021). Örneğin, çıkış öncesi herbisitlerin malçlama ile kombinasyonu konusunda diğer ülkelerde yürütülen bazı çalışmalar sonucunda yabancı ot mücadelesinde başarılı sonuçlar elde edilmiştir (Chen ve ark., 2013; Witcher & Pouder, 2020;Yu & Marble, 2022). Sonuç olarak, Sakarya ili dış mekân süs bitkileri üretim alanlarında yabancı otlar ile ilgili kalite ve iş gücü kayıplarının neden olduğu bazı sorunlar yaşanmaktadır ve üretim saksılarındaki yabancı otların mücadelesi kimyasal kullanmadan üretim sezonu boyunca elle yolma yöntemi ile yapılmaktadır. ...

Improving Pennsylvania Bittercress Weed Control Efficacy with Mulch and Herbicide in Containers

HortTechnology

... The vacant space helps weeds grow, resulting in nutrient depletion through weeds (Kaur et al. 2018). Besides, the allelopathic effect of weeds on the growth of the main crop has also been recorded (Yuvraj et al. 2022). Thus, intercrop provides shelter/ cover to the soil, suppresses weed growth, provides greater below-ground productivity than sole crops, and sequesters more soil carbon over time due to more significant root litter input (Cong et al. 2015). ...

Allelopathy and its application as a weed management tool: A review

... T. fluminensis has been vouchered in over 20 counties in Florida, primarily confined to central and north Florida [2]. Differences and similarities between the two species have been detailed previously [5,9,10]. In brief, both plants are spreading herbaceous groundcovers which root extensively along their nodes, have a high propensity for spreading via stem fragmentation, and form dense vegetative mats on the forest floor which alter the germination and growth of many understory species [4,6]. ...

Herbicide selection for controlling Tahitian bridal veil ( Gibasis pellucida )

Invasive Plant Science and Management

... All investigated treatments reduced the fresh broadleaved weed biomass when compared to the control. Also, other investigations of the application of herbicides in forestry found that using chemical measures reduced the biomass of weeds [42,43]. Although the measured fresh weed biomass was larger in the plots with manual weeding, it was not significantly different when compared with the fresh weed biomasses obtained in the herbicide treatments. ...

Practice in Nursery Weed Control—Review and Meta-Analysis

... Accordingly, P that is released from the fertilizer and leaches past the root zone may be adsorbed to the FSB before draining from the container. Growing containerized nursery and greenhouse crops in strategic layers of substrate, sometimes referred to as substrate stratification, has recently been proposed to decrease fertilizer requirements [30], reduce weed growth and weed seed germination [31,32], mitigate crop water stress during drought [33], and reduce peat use [34]. However, stratifying a substrate to include a P-sorbing base layer has not yet been explored. ...

Effect of Substrate Stratification on Growth of Common Nursery Weed Species and Container-grown Ornamental Species

HortTechnology