January 2000
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9 Reads
DNA sequencing methods, including both Maxam-Gilbert and Sanger di-Principle anddeoxy procedures, have traditionally involved the detection of DNA frag-applicationsments labeled with radioactive isotopes. More recently, other techniques for imaging DNA sequencing ladders with nonisotopic labels have become available. These include colorimetric BCIP and NBT (Richterich et al., 1989), fluorescent (Prober et al., 1988), and chemiluminescent (Beck et al., 1989; Tizard et al., 1990; Creasey et al., 1991; Martin et al., 1991; Bronstein et al., 1992) methods.