Ruth E. Richardson’s research while affiliated with Cornell University and other places

... [18][19][20][21][22][23][24] Many other previous works have also focused on characterizing machine learning classication algorithms for automated processing of spectroscopic datasets. 18,[25][26][27][28] Many of the established techniques, come with challenges. For example, PY-GC/MS is destructive to the samples, LIBS poses safety concerns due to the creation of plasma on plastics, and Raman spectroscopy is susceptible to uorescence background. ...

Reference:

Microplastics in the rough: using data augmentation to identify plastics contaminated by water and plant matter

... This suggests that PO 4 3− may be sequestered in the bioreactors, at least partially, via assimilation and intracellular storage, as also suggested in woodchip bioreactors enhanced by oxic-anoxic cycling. 127 Although this biological strategy can significantly enhance phosphorus removal from wastewater, 125 woodchip biofilms can also remove substantial amounts of PO 4 3− via precipitation on their extracellular polymeric matrix. 128 This strategy becomes progressively more relevant as the biofilms grow on the woodchip substratum and the surface area available for PO 4 3− precipitation increases. ...

Reference:

Control of Microbiome Composition and Agrichemical Remediation in Woodchip Bioreactors by Hydraulic Retention Time

... One of the most crucial environmental issues currently is the ubiquitous presence of microplastics in aquatic ecosystems (Koelmans et al., 2022;Vethaak and Legler, 2021;Zhao et al., 2024;Li et al., 2023). Their persistence in the environment and the harmful impacts on organisms, including circulation throughout the body, penetration of vital barriers, and the potential to cause oxidative damage and immunological stress (Vethaak and Legler, 2021;Li et al., 2024;Kadac-Czapska et al., 2024). ...

Reference:

Enhanced spectral signatures with Ag nanoarrays in hyperspectral microscopy for CNN-based microplastics classfication

... An additional insight is that data from open-water locations can still be effective at estimating shoreline cya-noHAB occurrence. Our findings add to a growing body of literature that uses data collected by the Citizens Statewide Lake Assessment Program (CSLAP) and NYSDEC to evaluate patterns and impacts of cyanoHABs (Gorney, June, et al., 2023;Wang et al., 2024;Wasswa et al., 2024). CSLAP was initiated to track water quality across New York State (Kishbaugh, 1988) but has been leveraged over nearly 40 years to broaden and address new issues as they emerge. ...

Reference:

Estimating indicators of cyanobacterial harmful algal blooms in New York State

... Based on a comparative analysis of the 16S rRNA gene sequence ( Figure S3), strain CWV2 was classified within the Victoria subgroup of Dehalococcoides mccartyi, which, along with Pinellas and Cornell, represented the three common subgroups (Morson et al., 2022). Remarkably, strain CWV2 demonstrated the ability to completely degrade PCE (45.6 ± 0.3 μmole) to ethene, with only a small amount of VC detected (2.9 ± 0.2 μmole) over 40 days (Fig. 1A). ...

Reference:

Mechanistic Insights into Chloroethene Dechlorination by Dehalococcoides mccartyi Strain CWV2: A Multi-Omics Perspective

... Cytochrome c L , in turn, is oxidized by a typical class I cytochrome c (cytochrome c H ), which is also specific for methanol oxidation (Kang-Yun et al. 2022). Importantly, all three components-MDH, cytochrome c L , and cytochrome c H -are soluble and reside within the periplasm of gramnegative methylotrophs (Tentori et al. 2022). In contrast, gram-positive methylotrophs employ an NAD-linked MDH for methanol oxidation, while methanol-oxidizing yeast species use a methanol oxidase system for the same purpose. ...

Reference:

Recent findings in methanotrophs: genetics, molecular ecology, and biopotential

... SRB inhibits methanogens through competition for substrates and electrons, inhibiting the production of methane. 166 (3) Sulfate-reducing bacteria consume organic carbon while reducing sulfate, and accelerate the inorganic mineralization of OM. 167 The inorganic mineralization of OM produces carbonate and combines with Ca 2+ in gypsum to form calcite, which improves the conversion rate of organic carbon to inorganic carbon. More experimental studies are needed to clarify the gypsum addition effects on the BCE C cyclings. ...

Reference:

Coastal blue carbon in China as a nature-based solution toward carbon neutrality

... Nucleic acids can be detected on-site using automated PCR. These systems have been explored particularly in coastal waters, but they are still in research and development phases (Fernández-Baca et al., 2021;Sepulveda et al., 2020;Yamahara et al., 2015). Recent developments demonstrate the feasibility of an automated on-site qPCR biosensor for high frequency monitoring of Legionella pneumophila in cooling towers (Trigui et al., 2024), however, there is currently no scientific literature available to substantiate its capability in drinking water contexts. ...

Reference:

Automation of on-site microbial water quality monitoring from source to tap: Challenges and perspectives

... This phylum is both metabolically diverse and has been found in a variety of environments, both oxic and anoxic. Members of this group have been reported to be directly and indirectly involved in fermentation in soils as well as being key players in sulfate reduction [51][52][53]. Their higher relative abundance in peatlands neighboring the smelter may relate to the higher contaminant concentrations (supp. Tables 1 and 4) and pH of these sites, suggesting a more active organic matter turnover. ...

Reference:

Recovery of Smelter-Impacted Peat and Sphagnum Moss: a Microbial Perspective

... Our results confirmed that bacterial taxa, particularly those in the classes Acidobacteria and Alphaproteobacteria, were most associated with protein-and carbohydrate-like compounds, whereas fungal taxa, including members of Ascomycota and Basidiomycota, showed strong associations with carbohydrateand lignin-like compounds. This is consistent with previous research suggesting that bacteria, particularly those in the Acidobacteria group, are more likely to degrade labile organic substrates (Wang et al. 2021c), while fungi are more specialized in decomposing recalcitrant, lignin-rich compounds (James et al. 2021;Ling et al. 2022). The ability of fungi to decompose complex lignin structures is particularly important for nutrient cycling in forest soils, as lignin is a major component of plant litter and a key source of carbon for soil microorganisms (Huang et al. 2021). ...

Reference:

Effects of DOM Chemodiversity on Microbial Diversity in Forest Soils on a Continental Scale