Ruth Ben-Arie’s research while affiliated with Migal - Galilee Technology Center and other places

The effect of ‘Hayward’ kiwifruit maturity at harvest on fruit quality during long-term storage at −0.5 °C was evaluated by harvesting the fruit several times, at different stages of maturity. The progress of maturation on the vine was monitored weekly from 136 DAFB (days after full bloom). Fruit were harvested for storage at three points and stored for 3–6 months in regular air (RA), or for 6–10 months in a controlled atmosphere (CA), with or without prestorage exposure to 1-methylcyclopropene (1-MCP). The softening rate under both storage regimes decreased with the advance in fruit maturation on the vine, as indicated by increasing soluble solids content (SSC), and declining firmness. As a result, the fruit from the first harvest (152 DAFB), which were the firmest at harvest, were the softest at the end of both storage regimes. Delaying harvest also decelerated the decline in acidity during storage, so that fruit picked last maintained the highest titratable acidity (TA) upon removal from storage. The overall fruit quality after shelf life, following prolonged storage in either RA or CA, was improved by delaying harvest to late November (ca. 200 DAFB). The harvest criteria for fruit with the best storage potential were dry matter (DM) > 17%, SSC > 7%, TA 2.0–2.6%, with more than 40% of the DM non soluble. From a commercial aspect the rule should therefore be ‘Last in, last out’ (LILO).

The physiology of fruit ripening is defined as either ‘climacteric’ or ‘non-climacteric’. In climacteric fruit respiration during ripening increases until it reaches a peak, which is accompanied by an increase in autocatalytic ethylene production, whereas the respiration of non-climacteric fruit does not increase and they have no requirement for ethylene to complete their ripening. In an attempt to gain further insight into the involvement of autocatalytic ethylene production with the climacteric rise in respiration, tomato fruit were harvested at three defined stages of maturity prior to the climacteric peak (mature green, breaker, and early orange) and immediately exposed to the gaseous molecule 1-methylcyclopropene (1-MCP). The gene expression profile at each of these stages was monitored after 24 h, using an Affymetrix tomato microarray chip. This approach enabled us to identify ethylene responsive genes that are commonly regulated at early stages of ripening, as well as new candidate genes. In addition, 1-MCP treatment affected the levels of metabolites related to methionine biosynthesis. Methionine feeds climacteric ethylene production and we found that promotors of the genes of enzymes that catalyze the production of homoserine and homocysteine (aspartokinase/homoserine dehydrogenases and cystathionine beta lyase, respectively), precursors in the methionine pathway, contain the AtSR1 binding motif. This binding motif is recognized by ethylene activated transcription factors, hence indicating a role for ethylene in methionine synthesis during early ripening, explaining the autocatalytic ethylene production during subsequent ripening stages.

Kiwifruit are commercially harvested when mature but firm. The length of the softening process in shelf life (SL) till the fruit becomes edible depends upon several pre-harvest and postharvest factors, such as harvest maturity, environmental conditions (temperature, relative humidity, composition of the atmosphere) and duration of cold storage. The ability to predict the time till softening would be of considerable commercial value, but to date there is no reliable method to do so. We assessed the suitability of the non-destructive measurement of fruit firmness with the Sinclair IQ Firmness Tester (SIQ) to predict the time required for adequate fruit softening for consumption. ‘Hayward’ kiwifruit were harvested at commercial maturity and stored in either regular air (RA) or controlled atmosphere (CA), with or without pre-storage 1-MCP treatment. Fruit softening was monitored during shelf-life at 20 °C by finger pressure, SIQ and with a FTA penetrometer. Highly significant correlations were obtained between SIQ and both firmness assessments. The linear decline in firmness measured by the non-destructive SIQ enables us to construct models that can predict the time till softening, based on the SIQ value at removal from storage under different conditions.