Russell M. Lebovitz's research while affiliated with Washington University in St. Louis and other places

Publications (3)

Article
The 5.2-kilobase (kb) genome of the autonomous parvovirus H-1 was transcribed in the rightward direction, yielding steady-state polyadenylated transcripts of 4.8, 3.2, and 2.9 kb. Detailed mapping of these transcripts demonstrated that the H-1 genome contained two overlapping transcription units: the larger unit extended from 4 map units (5' end) t...
Article
Full-text available
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription ini...
Article
We identified viral transcripts in parvovirus H1-infected rodent cells using the S1 nuclease mapping technique of Berk and Sharp (1977, 1978). The most abundant viral transcript, present in both nucleus and cytoplasm, is approximately 2.8 kb long and represents about 56% of the viral genome. Less abundant viral transcripts of 3.0, 1.45 and 1.30 kb,...

Citations

... Also, an analysis of cells harboring bovine papillomavirus vectors containing portions of the MVM genome shows that VP1 is produced in cell lines that do not express the Ri or R2 mRNAs (21). Our data indicate that the mRNA coding for VP1 closely resembles the R3 mRNA and could not have been distinguished from R3 with the rather long probes used in the initial mapping experiments (12,20). For this reason, we chose to call it R3'. ...
... H-1 parvovirus (H-1 PV) is a small rodent virus whose genome is a 5 kb single-stranded DNA organized in two overlapping transcriptional units controlled by early P4 and late P38 promoters (57,65,134,210). The first one depends on cellular factors for its activation and drives the expression of both non structural proteins, NS1 and NS2 (3,87,199,230), while the second one, mainly activated by NS1 itself, allows capsid proteins VP1 and VP2 to be generated (209). ...
... Nuclear extract from MS2-tagged RUS-expressing Neuro2A cells was prepared typically from 8 × 10 7 cells without dialysis, according to Dignam et al (1983). Extract preparation and MS2-affinity purification were carried out at 4°C. Cell pellets were suspended in 5 vol buffer A (10 mM Hepes, pH 7.9 at 4°C, 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT, and 200 U/ml RNAsin) and incubated for 10 min. ...