Rupert A. Collins's research while affiliated with University of Bristol and other places
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Publications (85)
Lanternfishes (Myctophidae) are one of the most species‐rich families of mid‐water fishes. They inhabit the mesopelagic zone, where physical barriers to dispersal and gene flow are permeable. Thus, modes of speciation that rely exclusively on geographical separation are potentially of less importance than those that rely more prominently on evoluti...
Many factors influence how environmental DNA (eDNA) abundance varies in natural environments. One of the least studied contributors to eDNA variation is that of reproduction. Marine organisms that broadcast spawn are expected to shed increased quantities of DNA in association with the release of gametes and the elevated levels of activity associate...
Molecular phylogenetic evidence clearly resolves the African cichlid fish genus Ctenochromis, as defined by Greenwood (1979), as paraphyletic. Here, we redefine the genus Ctenochromis and assign Ctenochromis horei, a member of the Tropheini from Lake Tanganyika, to a new genus Shuja gen. nov. We restrict Ctenochromis to Ctenochromis pectoralis and...
We present a genome assembly from an individual Trachurus trachurus (the Atlantic horse mackerel; Chordata; Actinopteri; Carangiformes; Carangidae). The genome sequence is 801 megabases in span. The majority of the assembly, 98.68%, is scaffolded into 24 chromosomal pseudomolecules. Gene annotation of this assembly on Ensembl has identified 25,797...
Abstract Many sharks, skates, and rays (elasmobranchs) are highly threatened by the activities of commercial fisheries, and a clear understanding of their distributions, diversity, and abundance can guide protective measures. However, surveying and monitoring elasmobranch species can be highly invasive or resource‐intensive, and utilization of non‐...
Aquatic invasive species, such as the signal crayfish (Pacifastacus leniusculus), present a major threat to freshwater ecosystems. However, these species can be challenging to detect in recently invaded habitats. Environmental DNA (eDNA)–based methods are highly sensitive and capable of detecting just a few copies of target DNA from non-invasively...
The accuracy and reliability of DNA metabarcoding analyses depend on the breadth and quality of the reference libraries that underpin them. However, there are limited options available to obtain and curate the huge volumes of sequence data that are available on public repositories such as NCBI and BOLD. Here, we provide a pipeline to download, clea...
Environmental DNA analysis is rapidly transforming biodiversity monitoring and bolstering conservation applications worldwide. This approach has been assisted by the development of metabarcoding PCR primers that are suited for detection of a wide range of taxa. However, little effort has gone into exploring the value of the non-target DNA sequences...
[This corrects the article DOI: 10.1371/journal.pntd.0008129.].
Pacus of the genus Myloplus represent a formidable taxonomic challenge, and particularly so for the case of M. asterias and M. rubripinnis, two widespread and common species that harbor considerable morphological diversity. Here we apply DNA barcoding and multiple species discovery methods to find candidate species in this complex group. We report...
Background
Schistosomiasis is a neglected tropical parasitic disease associated with severe pathology, mortality and economic loss worldwide. Programs for disease control may benefit from specific and sensitive diagnostic methods to detect Schistosoma trematodes in aquatic environments. Here we report the development of novel environmental DNA (eDN...
Marine environments have increased in temperature by an average of 1°C since pre-industrial (1850) times [1]. Given that species ranges are closely allied to physiological thermal tolerances in marine organisms [2], it may therefore be expected that ocean warming would lead to abundance increases at poleward side of ranges and abundance declines to...
Background:
Schistosomiasis is a neglected tropical disease that infects over 200 million people worldwide. Control measures can benefit from improved surveillance methods in freshwaters, with environmental DNA (eDNA) surveys having the potential to offer effective and rapid detection of schistosomes. However, sampling eDNA directly from natural w...
Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, and especially so when developing community standards and legislative frameworks, is the choice of genetic marker and primer set. Mitochondrial cytochrom...
Environmental DNA (eDNA) analysis, as a tool for assessing and monitoring aquatic animal distributions, depends on our understanding of biotic and abiotic factors that may affect the degradation and transport of water-borne DNA. The mechanisms of dispersal of cellular material and particle-bound DNA across open water systems, such as the ocean, rem...
Cichlids are a conspicuous component of Amazonian ichthyofauna, filling a wide range of niches. Yet taxonomy of many groups is still poorly known in the Amazon, and most of the yet-to-be discovered species are concentrated there. We analyzed 230 individuals sampled from six major Amazonian River Basins representing 56 morpho-species, 34 nominal and...
As environmental DNA (eDNA) becomes an increasingly valuable resource for marine ecosystem monitoring, understanding variation in its persistence across contrasting environments is critical. Here, we quantify the breakdown of macrobial eDNA over a spatio-temporal axis of locally extreme conditions, varying from ocean-influenced offshore to urban-in...
Piranhas and pacus (Characiformes: Serrasalmidae) are a charismatic but understudied family of Neotropical fishes. Here, we analyse a DNA barcode dataset comprising 1,122 specimens, 69 species, 16 genera, 208 localities, and 34 major river drainages in order to make an inventory of diversity and to highlight taxa and biogeographic areas worthy of f...
The rapids-dwelling suckermouth catfish genus Pseudolithoxus was previously only known from the Guiana-Shield-draining Orinoco and Casiquiare river systems of Colombia and Venezuela, but new records have expanded this range considerably further into the Amazon basin of Brazil, and include occurrences from rivers draining the northern Brazilian Shie...
In the age of genome-scale DNA sequencing, choice of molecular marker arguably remains an important decision in planning a phylogenetic study. Using published genomes from 23 primate species, we make a standardized comparison of four of the most frequently used protocols in phylogenomics, viz., targeted sequence-enrichment using ultraconserved elem...
The Asian (nandid) and Afro-Neotropical (polycentrid) leaffishes represent two superficially similar, but historically poorly diagnosed families – a situation resulting in a convoluted systematic history. Here, and including for the first time in a molecular study all leaffish genera, we generate a hypothesis of the phylogenetic history of both gro...
R script to access GBIF and FishBase records
The Rio Nhamundá is a poorly-known clearwater river draining the southern Guiana Shield of Brazil. In this study we report the findings of a preliminary ichthyological survey, focusing on catfishes (Siluriformes). We identify a total of 36 species (31 genera, seven families) from the Nhamundá, including 11 species already recorded from the river. O...
Bash script to search locally stored checklists in PDF format
The international trade in ornamental aquatic organisms represents an important vector in the spread of invasive species worldwide, but the accurate identification of imported organisms as part of a biosecurity surveillance program offers an opportunity to mitigate potential problems. Species level identification is historically conducted visually,...
DNA barcoding remains a challenge when applied to diet analyses, ancient DNA studies, environmental DNA samples and, more generally, in any cases where DNA samples have not been adequately preserved. Because the size of the commonly used barcoding marker (COI) is over 600 base pairs (bp), amplification fails when the DNA molecule is degraded into s...
1. DNA barcoding studies use Kimura's two-parameter substitution model (K2P) as the de facto standard for constructing genetic distance matrices. Distances generated under this model then provide the basis for most downstream analyses, but uncertainty in model choice is rarely explored and could potentially affect how reliably DNA barcodes discrimi...
NJ phylogram (COI data) of all specimens (this study plus GenBank/Bold data), in phyloXML SVG (scalable vector graphic) format. Archived version of Figure S1 may require open-source archiving software such as “7-Zip” to unpack. The interactive Web version can be found at http://goo.gl/avNuz. Data including identifiers, sequences, trace files, museu...
Text file containing all COI sequences used in the study (Fasta format).
(TXT)
NJ phylogram (reduced RHO data) generated in phyloXML SVG (scalable vector graphic) format. Archived version of Figure S2 may require open-source archiving software such as “7-Zip” to unpack. The interactive Web version can be found at http://goo.gl/h9sY5. Data including identifiers, sequences, trace files, museum voucher codes and specimen images...
Full list of specimens, identifications, morphological characters, comments, and bibliography of samples generated in this study.
(PDF)
Background:
Poorly regulated international trade in ornamental fishes poses risks to both biodiversity and economic activity via invasive alien species and exotic pathogens. Border security officials need robust tools to confirm identifications, often requiring hard-to-obtain taxonomic literature and expertise. DNA barcoding offers a potentially a...
Text file containing all RHO sequences used in the study (Fasta format).
(TXT)
Spider: SPecies IDentity and Evolution in R is a new R package implementing a number of useful analyses for DNA barcoding studies and associated research into species delimitation and speciation. Included are functions essential for generating important summary statistics from DNA barcode data, assessing specimen identification efficacy, and for te...
Citations
... Trachurus trachurus (Atlantic horse mackerel)[50][51][52][53].DATA VALIDATION AND QUALITY CONTROLSequencingCLR sequencing (PacBio) generated 3.74 M reads with a total of 58.25 Gbp, which is approximately 93× physical coverage of the genome. The mean and N50 read lengths were 15,591.278 ...
... Current methods for obtaining census data on cartilaginous fish are based on the visual identification of caught individuals, which is invasive and expensive, often speciesselective and dependent on taxonomic expertise (Boussarie et al., 2018). The recent development of environmental DNA (eDNA) offers a powerful, cost-effective, noninvasive alternative (Taberlet et al., 2012;Thomsen et al., 2012), which has been shown to be effective in tracking the presence of cartilaginous fish in various environments (Bakker et al., 2017;Boussarie et al., 2018;West et al., 2021;Dunn et al., 2022;Liu et al., 2022). Spatial models are continuously developed for studying the impact of demersal fisheries, and the spatial origin of catches, using satellite-based information on fishing activities (D'Andrea et al., 2020;Russo et al., 2018Russo et al., , 2019; eDNA could enhance these models, being itself an important source of biological information. ...
... Insights into the spatial distribution of AIS and patterns of secondary spread are essential in devising successful containment strategies (Vimercati et al. 2019;Araya-Donoso et al. 2022;Greenhalgh et al. 2022). However, conventional aquatic species surveillance can be labour-intensive and costly when conducted on vast spatiotemporal scales and when a priori knowledge of occurrence and ecological habitat preferences is unavailable (Mueller et al. 2017;Vimercati et al. 2019;Da Silva Neto et al. 2020). ...
... We first downloaded an accurate and updated list of Mediterranean chondrichthyan species following the steps available at github.com /genner-lab/meta-fish-lib (Collins et al., 2021). The list was then validated according to the recent literature (Serena et al., 2020;F. ...
... Some automation of both approaches has been achieved in field deployable remote automated underwater vehicles and there is significant capacity to improve this further 99 . Living filters such as mussels and other shellfish also concentrate exogenous nucleic acids and may be suitable for monitoring, including some in pre-existing populations, but this concept remains very much in early development 96,100 . ...
... However, we maintained the data for this species in our analysis (Table S2), as the interviewed fishers noticed the difference in the photograph shown and, although they provided information for the related species (filhote , Table S2), they alerted us that the fish shown is not usually found in the Tapajos River. In the Negro River, we included two photographs of the species Myloplus nigrolineatus (pacu galo, Table S1), which has morphological differences between females (Fig. S7a) and males ( Fig. S7b) (Ota et al. 2020, Silvano 2020. Nevertheless, at least half of the interviewed fishers in the Negro River accurately mentioned that these two fish would be f