Runjia Ji's research while affiliated with Chinese Academy of Sciences and other places
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Publications (2)
Magnetosome gene clusters (MGCs), which are responsible for magnetosome biosynthesis and organization in magnetotactic bacteria (MTB), are the key to deciphering the mechanisms and evolutionary origin of magnetoreception, organelle biogenesis, and intracellular biomineralization in bacteria. Here, we report the development of MagCluster, a Python s...
Magnetotactic bacteria represent a valuable model system for the study of microbial biomineralization and magnetotaxis. Here, we report two metagenome-assembled genome sequences of uncultivated magnetotactic bacteria belonging to the order Magnetococcales . These genomes contain nearly complete magnetosome gene clusters responsible for magnetosome...
Citations
... For comparative genomic analysis, a total of seven representative Nitrospirota MTB genomes and three marine MTB genomes were obtained from the NCBI GenBank database. The identification, annotation, and visualization of magnetosome gene clusters (MGCs) were performed using MAGcluster (Ji et al., 2022) with manual inspection. ...
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... To obtain sufficient DNA for metagenomic sequencing, whole-genome amplification was carried out using the multiple displacement amplification technique with the Genomiphi V2 DNA Amplification Kit (GE Healthcare, United States). This approach has been widely used previously in various works (Kolinko et al., 2015;Monteil et al., 2019;Zhang et al., 2020b). The amplified DNA was purified by sodium acetate precipitation. ...
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