Rui Xin’s scientific contributions

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Publications (1)


Figure 1: Peptide contents (<10 kDa) of the control (cow milk) and four varieties of CBW (namely white, black, kidney and cranberry) subjected to in vitro gastrointestinal simulated digestion (GIS): P0-before the GIS; P1-after buccal digestion; P2−5, P2−30, P2−60 represented samples taken at 5 min, 30 min, and 60 min of gastric digestion (P2); P3−5, P3−30, P3−120 represented samples taken at 5 min, 30 min, and 120 min of intestinal digestion (P3). Each value was expressed as mean ± standard deviation (n=3). Means with different small letters (a, b, c, d and e) indicated signifi cantly different (p < 0.05).
Figure 2: SDS-PAGE analysis of the control (cow milk) and four varieties of CBW (namely white, black, kidney and cranberry) before the GIS digestion (P0), during buccal (P1), gastric (P2: 5 min, 30 min, 60 min), and duodenal (P3: 5 min, 30 min, 120 min) phases of in vitro GIS digestion. (A) control; (B) white; (C) black; (D) kidney; (E) cranberry. The numbers in each picture from top to bottom represents α-CN, β-CN, κ-CN and β-LG subunits respectively.
Figure 3: DPPH radical scavenging effects (A), ABTS radical cation scavenging ability (B), ferric reducing antioxidant power (C), hydroxyl radical scavenging activity of the control and CBWFY after (P3-120) phase of duodenal digestion. Each value was expressed as mean ± standard deviation (n=3). Means with different small letters (a, b, c, d and e) indicated signifi cantly different (p < 0.05).
Figure 4: In vitro ACE inhibitory activity of control and CBWFY after (P3-120) phase of duodenal digestion. Each value was expressed as mean ± standard deviation (n=3). Means with different small letters (a, b, c and d) indicated signifi cantly different (p < 0.05).
Figure 5: Taste profi le (descriptive analysis) of common bean whey plus cow milk as control. Each value is expressed as mean ± standard deviation scored on a 9-cm line scale, ranging from 0 (detectable) to 9 (intense), by 9 panelists (n= 9, 3 x replicates), p < 0.05(ANOVA, Tukey's HSD).

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Antioxidant and ACE-Inhibitory Activity of Common Bean Whey Fortified Yoghurt with Assessed by in vitro Static Gastrointestinal Digestion
  • Article
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March 2020

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239 Reads

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3 Citations

International Journal of Agricultural Science and Food Technology

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Rui Xin

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Dong Mingsheng

Yogurt supplemented with plant source proteins are received increased attention but few studies investigated effect of plant source proteins supplementation on protein digestibility and releasing of bioactive peptides. In this study, a residue rich in protein derived from common beans starch production, Common Bean Whey (CBW), was applied in development of novel yogurt. CBW from four major common beans varieties, namely white, black, kidney and cranberry were utilized. The novel yogurts were subjected to in vitro Gastro-Intestinal Simulation (GIS) digestion and protein digestibility, antioxidant activity and Angiotensin I Converting Enzyme (ACE) inhibitory property were studied. Compared to the control, Common Bean Whey Fortifi ed Yogurt (CBWFY), particularly black bean whey was the predominate stable sample to protein degradation in the gastric and intestinal phases. Peptide content and SDS-PAGE revealed that CBWFY samples exhibited mostly lower hydrolysis grades in gastric and commence of duodenal phases. High antioxidant and ACE inhibitory activities results were attributed to CBWFY, in which signifi cantly (p < 0.05) higher value was observed in kidney bean whey digesta at120min phase of intestinal digestion. For the fi rst time, the outcomes of this investigation demonstrated the infl uence of four varieties of common beans whey supplementation on protein hydrolysis kinetics, digestive degree, antioxidant activity and ACE inhibitory properties on yogurt.

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Citations (1)


... During food processing, the pH of the food not only inhibits the growth of microorganisms but also influences chemical reactions (Moosavy et al. 2017). The growth of pathogenic bacteria is restricted by pH levels below 4.6, and the Maillard reaction does not occur in acidic conditions Zahir et al. 2020). In addition to pH, the moisture content of the food is also a crucial factor in AA formation. ...

Reference:

Food process contaminants: formation, occurrence, risk assessment and mitigation strategies–a review
Antioxidant and ACE-Inhibitory Activity of Common Bean Whey Fortified Yoghurt with Assessed by in vitro Static Gastrointestinal Digestion

International Journal of Agricultural Science and Food Technology