Rosario Martín-Orti’s research while affiliated with Complutense University of Madrid and other places

The study of the brain by magnetic resonance imaging (MRI) allows to obtain detailed anatomical images, useful to describe specific encephalic structures and to analyze possible variabilities. It is widely used in clinical practice and is becoming increasingly used in veterinary medicine, even in exotic animals; however, despite its potential, its use in comparative neuroanatomy studies is still incipient. It is a technology that in recent years has significantly improved anatomical resolution, together with the fact that it is non-invasive and allows for systematic comparative analysis. All this makes it particularly interesting and useful in evolutionary neuroscience studies, since it allows for the analysis and comparison of brains of rare or otherwise inaccessible species. In the present study, we have analyzed the prosencephalon of three representative sauropsid species, the turtle Trachemys scripta (order Testudine), the lizard Pogona vitticeps (order Squamata) and the snake Python regius (order Squamata) by MRI. In addition, we used MRI sections to analyze the total brain volume and ventricular system of these species, employing volumetric and chemometric analyses together. The raw MRI data of the sauropsida models analyzed in the present study are available for viewing and downloading and have allowed us to produce an atlas of the forebrain of each of the species analyzed, with the main brain regions. In addition, our volumetric data showed that the three groups presented clear differences in terms of total and ventricular brain volumes, particularly the turtles, which in all cases presented distinctive characteristics compared to the lizards and snakes.

Simple Summary Anatomy is considered critical to understanding the size and shape of the different systems of animals belonging to the same group or individuals that are close in the phylogenetic scale. In the case of the Franciscana dolphin or Pontoporia blainvillei, the digestive apparatus shows some differential characteristics compared to other dolphins or other marine mammals. In the present work, the authors try to demonstrate whether these characteristics are conditioned by the animal’s own diet or if they have more to do with certain phylogenetic adaptations. Considering that the Franciscana dolphin is an endangered species, any work that could facilitate better knowledge of these individuals has a high social, ecological, and scientific value because it will help in their care and conservation, and this is of utmost importance to implement effective management strategies for this species. Abstract The Franciscana (also known as the La Plata River Dolphin) is a small dolphin that lives in the coastal waters of Brazil, Uruguay, and Argentina. This species is considered the most endangered marine mammal in the western South Atlantic Ocean. Anatomic dissection of the digestive system of 19 animals of different ages, including 2 neonates, 12 juveniles, and 5 adults, was performed. Parameters related to length, breadth, weight, and diameter of the digestive viscera were considered in each case. Our results show that the Franciscana dolphin presents differential characteristics in relation to several parts of the digestive system, including, specifically, the tongue, the teeth, the stomach, and the small intestine. Thus, this paper add precious information to the actual knowledge of this vulnerable marine mammal species in order to improve conservation efforts.

Simple Summary Anatomy is regarded as a key element in medical and veterinary education all over the world. It is a subject of voluminous and sometimes complex content, which normally causes students’ different difficulties. These difficulties increase dramatically when it comes to the study of neuroanatomy. Hence, there have been continued efforts in developing new methods of teaching, learning and assessment that are aimed at the long-term retention of anatomical and neuroanatomical knowledge. Additionally, clinical neurology can be difficult for veterinary students to comprehend, and there is no doubt that part of its understanding is directly related to the knowledge of neuroanatomy. Therefore, the aim of this present work is to be a teaching tool oriented towards clinical neurology and clinical practice, through 3D reconstructions of magnetic resonance images of normal brains and clinical neurological cases. Abstract Neuroanatomy is always a challenging topic for veterinary students. It is widely accepted that understanding the anatomy of the central nervous system (CNS) is essential to explain many of the pathological processes that affect the brain. Although its study has varied over time to achieve this goal, in human and veterinary medicine it is difficult to find a teaching method that associates normal anatomy with pathological alterations of the brain. For the first time, we have created an educational tool that combines neuroanatomy and neuropathology, using different magnetic resonance (MR) images as a basis and EspINA software as analyzer, to obtain segmented structures and 3D reconstructions of the dog brain. We demonstrate that this combination is an optimal tool to help anatomists to understand the encephalon, and additionally to help clinicians to recognize illness including a multitude of neurological problems. In addition, we have tried to see whether photogrammetry, which is a common technique in other sciences, for example geology, could be useful to teach veterinary neuroanatomy. Although we still need further investigations, we have been able to generate 3D reconstructions of the whole brain, with very promising results to date.

Simple Summary It is normally recognized that anatomy is crucial for an improved knowledge of many physiological adaptations. In the case of marine mammals, their ability to dive for long periods of time is especially striking. They spend most of their lives in water, although in general terms, they behave and have characteristics very similar to those of land mammals. Our results demonstrate that, due to its capacity to stay under water, the respiratory apparatus of the South American fur seal shows specific characteristics. Yet, in general terms, being a carnivore seems to be the main characteristic of this species, and the adaptations to the aquatic environment being less important. Abstract Marine mammals are divided into three groups, with similar adaptations resulting from their aquatic lifestyle: sirenians, pinnipeds, and cetaceans. The present work focused on the South American fur seal, or Arctocephalus australis, a carnivore included in the pinnipeds group. We assessed whether the anatomical features of the Arctocephalus australis’ respiratory system are comparable to those of other land-carnivores or whether these individuals show anatomical adaptations related to their ability to dive or their breath-holding capacities. We studied 11 cadavers of Arctocephalus australis, which included adult (n = 2) and juvenile (n = 9) individuals, by anatomically dissecting their isolated entire respiratory system. Although it is generally similar to that in land-carnivores, we demonstrated that the Arctocephalus australis’s respiratory apparatus shows several specific characteristics. Therefore, our results are of great importance for clinical diagnostic and wildlife conservation purposes.

Simple Summary Marine mammals are warm-blooded vertebrates that behave in the same way as land mammals do but they spend most or all of their lives in the ocean. There is much previous research on whales, dolphins, or even different types of seals, including their behavior, health, anatomy or perception. Between all these fields, it is commonly accepted that anatomy is considered critical to understanding many physiological adaptations. For example, their ability to dive by holding their breath underwater for long periods of time. During this process they can postpone digestion for several hours. However, and contrary to what might be expected, our results show that being a carnivore seems to be the critical characteristic defining their digestive apparatus, the adaptations to the aquatic environment being less significant. Abstract Marine mammals play a critical ecological role as both predator and prey. They are divided into three groups that share similar adaptations to their aquatic life, but that have very different origins and life patterns: sirenians, pinnipeds, cetaceans. The species object of our interest is the South American fur seal or Arctocephalus australis, a carnivore classified within the group of pinnipeds. The objective of the present study was to evaluate whether the anatomical characteristics of the Arctocephalus australis’ digestive system are similar to that of other land-carnivores or if, on the contrary, this species shows anatomical adaptations related to their life in the ocean. The study was carried out on 11 cadavers of the species Arctocephalus australis, made up of two adults and nine juveniles, by means of the anatomical dissection of their entire isolated digestive system. We demonstrate that, with several exceptions, the anatomical characteristics of the digestive system of the Arctocephalus australis are similar to those in other carnivores. Therefore, our data constitute an important contribution for clinical diagnostic and conservation purposes, for both veterinarians and biologists.