Roger Dumke's research while affiliated with Technische Universität Dresden and other places
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Publications (75)
Analysis of illicit drugs, medicines, and pathogens in wastewater is a powerful tool for epidemiological studies to monitor public health trends. The aims of this study were to (i) assess spatial and temporal trends of population-normalized mass loads of illicit drugs and nicotine in raw wastewater in the time of regulations against SARS-CoV-2 infe...
Purpose: Analysis of illicit drugs, medicines and pathogens in wastewater is a powerful tool for epidemiological studies to monitor public health trends. The aims of this study were to (i) assess spatial and temporal trends of population-normalized mass loads of illicit drugs and nicotine in raw wastewater in the time of regulations against SARS-Co...
In the ongoing SARS-CoV-2 pandemic, there is a need for new strategies for surveillance and identification of arising infection waves. Reported cases of new infections based on individual testing are soon deemed inaccurate due to ever changing regulations and limited testing capacity. Wastewater based epidemiology is one promising solution that can...
Dependent on the excretion pattern, wastewater monitoring of viruses can be a valuable approach to characterizing their circulation in the human population. Using polyethylene glycol precipitation and reverse transcription-quantitative PCR, the occurrence of RNA of SARS-CoV-2 and influenza viruses A/B in the raw wastewater of two treatment plants i...
Wastewater-based epidemiology provides a conceptual framework for the evaluation of the prevalence of public health related biomarkers. In the context of the Coronavirus disease-2019, wastewater monitoring emerged as a complementary tool for epidemic management. In this study, we evaluated data from six wastewater treatment plants in the region of...
Mycoplasma pneumoniae and Mycoplasma genitalium are cell wall-less bacteria with strongly reduced genome content and close phylogenetic relatedness. In humans, the only known natural host, the microorganisms colonize the respiratory or genitourinary mucosa and may cause a broad range of clinical presentations. Besides fundamental differences in the...
Background
Mycoplasma pneumoniae respiratory infections are transmitted by aerosol and droplets in close contact.
Aim
We investigated global M. pneumoniae incidence after implementation of non-pharmaceutical interventions (NPIs) against COVID-19 in March 2020.
Methods
We surveyed M. pneumoniae detections from laboratories and surveillance systems...
The treatment of infections from the sexually transmitted pathogen Mycoplasma genitalium is hampered by the rapidly increasing resistance to the recommended first- (macrolides) and second-line antibiotics (quinolones). Thus, resistance-guided therapy (RGT) is key for its successful eradication but the efficiency of this approach can be influenced b...
Background: Factors leading to the wide range of manifestations associated with Mycoplasma pneumoniae ( Mp ) infection are unclear. We investigated whether Mp genotypes are associated with specific clinical outcomes.
Methods: We compared Mp loads and genotypes of children with mucocutaneous disease to those with pneumonia, family members with upper...
We used multi-locus variable-number of tandem repeat analysis (MLVA), p1, multi-locus sequence (MLS) and single nucleotide polymorphisms (SNP) typing to characterize a collection of Mycoplasma pneumoniae strains from Cuba and Germany. Among 67 strains, 5 p1, 7 MLVA, 11 MLS, and 11 SNP types were obtained.
Ureaplasma urealyticum and U . parvum are mollicutes species that colonize the urogenital tract of many asymptomatic persons but are also thought to be associated with symptomatic infections. Using 170 strains isolated between 2016 and 2019 in a German university hospital, resistance was tested by a combination of commercial tests, molecular method...
Mycoplasma pneumoniae (M. pneumoniae) is a common causative pathogen of community-acquired pneumonia. Here, we report the development of macrolide resistance during a school outbreak of severe M. pneumoniae infections in southwest Germany. We conducted a case series to assess the clinical and laboratory characteristics of hospitalized children with...
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus...
Die Klasse der Mollicutes („Weichhäutigen“) umfasst Bakterien, die sich durch die Besonderheit einer komplett fehlenden genetischen Ausrüstung zum Aufbau einer Zellwand von den gramnegativen und grampositiven Bakterien unterscheiden. Mollicutes sind ähnlich wie eukaryontische Zellen nur durch eine Zytoplasmamembran begrenzt. Aufgrund der fehlenden...
Bacteria evolved many ways to invade, colonize and survive in the host tissue. Such complex infection strategies of other bacteria are not present in the cell-wall less Mycoplasmas. Due to their strongly reduced genomes, these bacteria have only a minimal metabolism. Mycoplasma pneumoniae is a pathogenic bacterium using its virulence repertoire ver...
Mycoplasma pneumoniae is a genome reduced pathogen and causative agent of community acquired pneumonia. The major cellular adhesin, P1, localises to the tip of the attachment organelle forming a complex with P40 and P90, two cleavage fragments derived by processing Mpn142, and other molecules with adhesive and mobility functions. LC-MS/MS analysis...
Background
Rectal sexually transmitted infections (STI) are common in men having sex with men (MSM). Mycoplasma genitalium is increasingly being reported in this localization, but due to frequent lack of symptoms at this site, clinical significance is still unclear. Rectal prevalence of Mycoplasma hominis and Ureaplasma species is not well studied...
Background
Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia, with large epidemics previously described to occur every 4 to 7 years.AimTo better understand the diagnostic methods used to detect M. pneumoniae; to better understand M. pneumoniae testing and surveillance in use; to identify epidemics; to determine detection numb...
Objectives:
The cell-wall-less Mollicutes species Mycoplasma genitalium is a sexually transmitted micro-organism that causes different male and female genital tract infections. In recent years, resistance of the pathogen to macrolides and fluoroquinolones has been increasingly reported worldwide and is more frequent in risk groups.
Methods:
To d...
Mycoplasma pneumoniae is a frequent cause of infections of the lower and upper respiratory tract of humans.…
Objectives: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia in humans. Treatment of infections can be complicated by the occurrence of macrolide resistant strains. The study was conducted to evaluate the presence of resistant strains in Cuba and to determine the corresponding genotypes. Methods: DNA of M. pneumoniae isolates...
Mycoplasma pneumoniae is a frequent cause of community-acquired infections of the human respiratory tract. During the evolutionary adaptation of the bacteria to the host, the genome of the pathogen is strongly reduced resulting in the loss of cell wall, limited metabolic pathways and a relatively small number of virulence factors. As interacting wi...
Many bacterial moonlighting proteins were originally described in medically, agriculturally, and commercially important members of the low G + C Firmicutes. We show Elongation factor Tu (Ef-Tu) moonlights on the surface of the human pathogens Staphylococcus aureus (SaEf-Tu) and Mycoplasma pneumoniae (MpnEf-Tu), and the porcine pathogen Mycoplasma h...
Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.
Mycoplasma pneumoniae is a common cause of community-acquired infections of the human respiratory tract. The strongly reduced genome of the cell wall-less bacteria results in limited metabolic pathways and a small number of known virulence factors. In addition to the well-characterized adhesion apparatus and the expression of tissue-damaging substa...
Mycoplasma pneumoniae is a major cause of community-acquired respiratory infections worldwide. Due to the strongly reduced genome, the number of virulence factors expressed by this cell wall-less pathogen is limited. To further understand the processes during host colonization, we investigated the interactions of the previously confirmed surface-lo...
Among 323 specimens from male patients with symptoms of non-gonococcal urethritis, . Mycoplasma genitalium was detected in 19 samples by real-time PCR. Mutations of 23S rRNA gene associated with macrolide resistance were confirmed in 10 strains. Amino acid changes at positions 81 and 83 of ParC protein were demonstrated indicating quinolone resista...
In humans of all ages, the cell wall-less and genome-reduced species Mycoplasma pneumoniae can cause infections of the upper and lower respiratory tract. The well-documented occurrence of major peaks in the incidence of community-acquired pneumonia cases reported world-wide, the multifaceted clinical manifestations of infection and the increasing n...
In different bacteria, primarily cytosolic and metabolic proteins are characterized as surface-localized and interacting with different host factors. These moonlighting proteins include glycolytic enzymes and it has been hypothesized that they influence the virulence of pathogenic species. The presence of surface-displayed glycolytic enzymes and th...
Since a well-documented incidence peak in 2011/12 in European countries, infections due to the cell wall-less bacterium Mycoplasma pneumoniae have gained the increased attention of clinicians, microbiologists and health authorities. Despite the mild or asymptomatic clinical course of most M. pneumoniae infections, the microorganism is responsible f...
The dual role of glycolytic enzymes in cytosol-located metabolic processes and in cell surface-mediated functions with an influence on virulence is described for various micro-organisms. Cell wall-less bacteria of the class Mollicutes including the common human pathogen Mycoplasma pneumoniae possess a reduced genome limiting the repertoire of virul...
Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem-repeat analysis (MLVA) is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six international laboratories to perform MLVA and results were compared....
Mycoplasma pneumoniae and Chlamydia spp., which are associated with community-acquired pneumonia (CAP), are difficult to propagate, and can cause clinically indistinguishable disease patterns. During 2011-2012, we used molecular methods to test adult patients in Germany with confirmed CAP for infection with these 2 pathogens. Overall, 12.3% (96/783...
The development of macrolide resistance that occurred during 3 days of therapy with azithromycin to treat Mycoplasma pneumoniae pneumonia in a paediatric patient is reported. After extended molecular characterization of strains, the parallel occurrence of clones showing the non-mutated wild-type 23S rRNA sequence as well as mutations A2063G and A20...
Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold
values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing
the best clinical sensitivit...
The attachment organelle of the human respiratory tract pathogen Mycoplasma pneumoniae is essential for colonization of the host mucosa. Furthermore, adherence-related proteins such as the major adhesin P1 and protein P30 represent vaccine candidates. Using the chimeric recombinant protein HP14/30, which combines surface-localized and adherence-inv...
Mycoplasma pneumoniae is a common cause of a broad spectrum of human respiratory tract infections which can be followed by extra-pulmonary complications.…
The obligate pathogenic mycoplasma species Mycoplasma pneumoniae uses a limited but effective repertoire of virulence factors to infect and colonize the human respiratory tract. Besides the development of an unique adhesion complex and the expression of tissue-damaging factors, surface-located glycolytic enzymes and their capacity to bind to compon...
Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our immunoblotting approach having...
A PCR approach for multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae directly from respiratory samples was developed and evaluated on 54 specimens from M. pneumoniae-positive pneumonia patients. The method resulted in a clearly identifiable MLVA type in all samples tested and can be used for MLVA without laborious i...
In different, phylogenetically unrelated micro-organisms, glycolytic enzymes play a dual role. In the cytosol they are involved in metabolic reactions whereas the surface-localized fraction of the enzymes contributes to adhesion and virulence. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a typical member of this group of multifunctional prot...
Mycoplasma pneumoniae is a frequent cause of community-acquired pneumonia. Three subtypes and three variants of M. pneumoniae have been described showing sequence differences in the main P1 adhesin. Between 2003 and 2006 we collected respiratory tract samples of adult outpatients with symptoms of pneumonia in a German nationwide network and detecte...
Legionella-like isolates, strains W03-356(T), W03-357 and W03-359, from three independent water samples from the river Elbe, Germany, were analysed by using a polyphasic approach. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut glass colony appearance that grew only...
The cell wall-less bacterium Mycoplasma pneumoniae is one of the most common agents of respiratory tract diseases in humans. Adhesin-mediated binding of the bacteria to host
cells is a crucial step in colonization and subsequent pathogenesis. For the first time, we expressed 16 recombinant proteins
covering almost the whole major adhesin P1 and the...
Clin Microbiol Infect 2010; 16: 613–616
In a total of 167 respiratory tract specimens from adult outpatients with confirmed Mycoplasma pneumoniae pneumonia, sampled between 2003 and 2008, and a further 99 isolates obtained from patients between 1991 and 2009 in Germany, M. pneumoniae was tested for macrolide resistance. Using PCR, real-time PCR and...
Raman spectroscopy has previously been demonstrated to be a highly useful methodology for the identification and/or typing of micro-organisms. In this study, we set out to evaluate whether this technology could also be applied as a tool to discriminate between isolates of Mycoplasma pneumoniae, which is generally considered to be a genetically high...
We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/μl, but the mean crossing points resulted in differences in the concentration
of the genome copies of a factor of 20.
Distinct sequence differences within the repetitive elements (RepMP) of the Mycoplasma pneumoniae P1 adhesin are the only targets to discriminate patient isolates with molecular approaches into subtypes and variants. Since the P1 protein is also one of the most immunodominant proteins of the bacterium, the antigenic regions of the differing repetit...
The protein Mpn474 encoded by the mpn474 gene of the human-pathogenic Mycoplasma pneumoniae contains 1033 amino acids and has an isoelectric point of 4.79, which is caused by the large excess of glutamic acid residues (11 %). Although the protein lacks recognizable export signals we showed by immuno-electron microscopy that Mpn474 is surface expose...
To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we
found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1...
Mycoplasma pneumoniae is one of the most common pathogens that causes community-acquired respiratory tract infection. Outbreaks are well known, and all age groups are susceptible. An outbreak in an army training unit afforded an opportunity to identify possible risk factors for morbidity.
An outbreak of respiratory illness that occurred in a unit c...
To evaluate the occurrence and abundance of phages that carry the stx(1) and stx(2) gene in water samples of different quality.
Phages growing on the Shiga toxin-negative Escherichia coli O157:H7 (ATCC 43,888) strain were enumerated by a plaque assay in concentrated raw and treated waste water samples and river water samples. Plaques were investiga...
In order to assess the risk of transmission of viral diseases during floods, the viral burden in flooded areas of the city of Dresden (Germany) in August 2002 was investigated. Water samples were collected from 9 sampling sites and tested for the presence of 11 enteric viral pathogens. As a control, water samples from the same sites were analyzed i...
A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region
of the P1 gene of Mycoplasma pneumoniae. It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and e...
A German mining lake and the supplying surface waters, which are located downstream of a sewage plant, were examined regarding their microbiological and virological quality. Between October 2002 and September 2003, specific PCR methods were used to determine the occurrence of enteric viruses in 123 water specimens drawn at different sites downstrea...
The genes P1 (MPN141) and ORF6 (MPN142) are essential for the successful colonization of the human respiratory tract by Mycoplasma pneumoniae. Both genes are located in the P1 operon, which consists of three genes. The P1 gene is the second gene in the operon, followed by the ORF6 gene. The P1 gene contains two (RepMP2/3, RepMP4) and the ORF6 gene...
The interaction between Mycoplasma pneumoniae and its natural host, humans, cannot be studied directly for obvious reasons. Therefore, we used guinea pigs instead, which had been recently introduced as an acceptable alternative host organism. The following experimental approaches were taken to study the pathogen-host relationship: characterization...
Mycoplasma pneumoniae isolates from patients, collected over a period of 12 years in Germany, were characterized by various methods (parameters) including multilocus sequence typing, restriction fragment length polymorphisms, Western blotting with mono-specific antibodies directed against selected proteins or with polyspecific antibodies directed a...
Citations
... In Germany, Agrawal et al. monitored the time course of viral RNA concentrations in untreated sewage in Frankfurt and demonstrated the potential of WBE as an early surveillance system for SARS-CoV-2 infection to identify global COVID-19 hotspots (Agrawal et al. 2021). Dumke et al. frequently detected SARS-CoV-2 and influenza viruses in 2 German WWTPs and concluded wastewater monitoring can be used to track disease epidemiology(Dumke et al. 2022). Randazzo et al. tested viral levels of SARS-CoV-2 in 6 WWTPs for the Murcia region and found that SARS-CoV-2 RNA could be detected in wastewater before municipalities reported confirmed cases, and argued that municipalities can use this environmental monitoring to make decisions to gradually lift the blockade measures during COVID-19 pandemic(Randazzo et al. 2020). ...
... The current activities to establish an early warning system for SARS-CoV-2 epidemic peaks has brought WBE strongly into focus (Erickson et al. 2021;Price et al. 2022, Wainwright et al. 2020Bade et al. 2021;Been et al. 2021;Cisneros and Cunningham 2021;Manchikanti et al. 2021;Palamar et al. 2021;Reinstadler et al. 2021;Helm et al. 2022). The study presented here deals with characterization of changes in the consumption patterns of illicit drugs and nicotine in a project for SARS-CoV-2-tracking in Germany (Helm et al. 2022). ...
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... Modellers have also exploited the detected viral load in their SEIR-based model in order to calibrate it 11 . Statistical regressions have been performed in order to compute reported cases based on the measured viral load 12 . The challenge that arises is, that both the wastewater data and the number of reported cases is very uncertain. ...
... 6 Compared with the pre-pandemic incidence of M pneumoniae (8⋅61%, 2017-20), a significant reduction was observed in the first year after the implementation of NPIs (1⋅69%, 2020-21), 6 similar to the incidence of other respiratory pathogens. 7 A further unprecedented, yet substantial, reduction in the incidence of M pneumoniae was observed in the second year (0⋅70%, 2021-22), 8 when other respiratory pathogens resurged as an indicator of community transmission. 8,9 The first global prospective surveillance study of M pneumoniae (ESGMAC MAPS study) 10 was initiated in April 2022 to allow for rapid notification regarding the geographical location of any substantial increase in activity via monthly website updates alerting clinicians. ...
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... It is unclear why epidemics occur in cyclic patterns, but one theory links this phenomenon to an alternation of Mp genotypes [1]. Mp isolates can be classified into two major genetic groups, designated subtype 1 and subtype 2, based on sequence differences in repetitive elements RepMP2/3 and RepMP4 in the P1 protein gene [1,41]. It has been speculated that the cyclical Mp epidemics that tend to occur every few years can be related to a shift from one P1 subtype to the other since the two major subtypes are immunologically distinct and exposure to one subtype may induce transient herd immunity that suppresses infection with that subtype while allowing the other one to reemerge [1]. ...
... The most recent epidemic occurred in late 2019-early 2020 simultaneously across multiple nations, predominantly in Europe and Asia. 6 In March, 2020, the introduction of non-pharmaceutical interventions (NPIs) against COVID-19 resulted in an abrupt ending of these epidemics and a marked decline in M pneumoniae detection worldwide. 6 Compared with the pre-pandemic incidence of M pneumoniae (8⋅61%, 2017-20), a significant reduction was observed in the first year after the implementation of NPIs (1⋅69%, 2020-21), 6 similar to the incidence of other respiratory pathogens. ...
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... In the African region, the rate of mutations connected with macrolide resistance in clinical isolates of ureaplasmas was near total at 99.5% (95% CI: 92.6-100). Comparatively, in European countries and the WHO Western Pacific region, this rate was considerably lower at 30 3), signaling a serious concern for health authorities. Regrettably, due to insufficient information, we could not calculate the rate of primary treatment failure among MDR Ureaplasma infections. ...
... Mp strains can be classified into different genotypes by using several typing methods, most frequently P1 typing, multilocus variable-number tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST) [11,12]. P1 typing separates the isolates into two major subtypes, P1 type 1 and P1 type 2, according to nucleotide differences in two repetitive elements (RepMP2/3 and RepMP4) in the MPN141 gene that codes for the P1 adhesion protein [13][14][15]. ...
... According to the difference of the RepMP2/3 and RepMP4 repetitive elements in the P1 protein gene, MP is roughly divided into two genetic groups, subtype 1 and 2 (Hsieh et al. 2022;Meyer Sauteur et al. 2021). Cell adhesion and mobility are the most important intrinsic virulence factors of MP. ...
... This hypothesis is supported by the results of studies reporting a decrease in the resistance rate after genotype change (Nakamura et al., 2021), followed by a subsequent increase among strains of the previously nondominant genotype (Wang et al., 2021). In small-scale outbreaks, time-dependent emergence of resistance among strains of the same genotype can be found (Hubert et al., 2021), emphasizing the need for mutation analysis in cases of treatment failure. ...
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