Robert Opiro's research while affiliated with Gulu University (GU) and other places

Publications (18)

Article
Shimba Hills is a wildlife area in Kenya and a major focus of tsetse-borne trypanosomes in East Africa. In Shimba Hills, tsetse-borne trypanosomes constrain animal health and smallholder livelihoods. However, epidemiological data to guide hotspot-targeted control of infections are limited. This study assessed the dynamics of tsetse-borne trypanosom...
Preprint
Full-text available
The primary vector of the trypanosome parasite causing human and animal African trypanosomiasis in Uganda is the riverine tsetse fly Glossina fuscipes fuscipes ( Gff ). We conducted a genome-wide association (GWA) analysis with field-caught Gff . To increase statistical power, we first improved the Gff genome assembly with whole genome 10X Chromium...
Data
Pairwise FST and ɸST comparisons for microsatellites (A) and mtDNA (B) respectively. FST values are reported in the lower diagonal. Since most values are significant, we highlight those that are non-significant in bold. All computations were done in ARLEQUIN. Significance was calculated based on a P<0.05. (XLSX)
Data
Total number of microsatellite alleles by locus. (DOCX)
Data
Microsatellite loci information. The table reports loci names followed by the forward (F) and reverse (R) primers names and sequences. The last column reports its source. M13 tails are marked with an asterisk (*). (DOCX)
Data
Table showing list and frequency of distribution of haplotypes recovered from the G. f. fuscipes samples mtDNA sequences analyzed from northern Uganda. (XLSX)
Data
Effective population size and bottleneck tests. Estimates of effective population size (Ne) were computed for each of the 42 sampling sites across the geographic regions in northern Uganda using three methods: LD, modified temporal method of Waples [66] based on [67] and heterozygote excess method. Estimates are provided together with their 95% CI....
Data
Discriminant Analysis of Principal Components (DAPC) based on genetic diversity at 16 microsatellite loci in 42 populations and obtained using the adegenet package [52] in R [53]. Two linear discriminants (LD1 and LD2) were used, following selection of principal components using a-score optimization, to plot G. f. fuscipes genotypes. Color codes ar...
Data
Sample information and molecular diversity indices. Sample geographic locations, sample sizes, and genetic diversity statistics for 16 microsatellite loci and for a 490bp mtDNA COI-COII gene fragment in 42 populations of G. f. fuscipes. ** Indicates samples collected prior to 2014, N = number of individuals analyzed, AR = Allelic richness, Ho = Obs...
Data
Table showing the total number of migrants based on GENECLASS and FLOCK. General information is displayed (population, closest village, drainage basin). Total, female, and male migrants are shown as counts from each unit based on both analyses. (XLSX)
Data
Linear regression of allelic richness (microsatellite loci) and haplotype diversity (mtDNA) over longitude produced using JMP V11.0 (SAS Institute Inc., Cary, NC, USA, 1989–2007). Triangles, diamonds, and squares identify sampling sites within the Northwest, Transition Zone, Northeast genetic units, respectively. (PDF)
Data
Table shows the probability of assignment (q-values) of individuals to each of the 3 genetic units, individual mtDNA haplotype, home region of individual, and if it’s a migrant the origin of migration, as well as comparison between microsatellite and mtDNA genetic assignment. (XLSX)
Data
Results of tests for isolation by distance where geographic distance between sampling sites (km) were linear-regressed over nuclear microsatellites based genetic distance (FST /(1-FST)) and mtDNA sequence based genetic distance (ΦST/(1-ΦST)). Results for the Northwest, Transition Zone, Northeast, West units, and all samples combined (Overall) are s...
Data
Wilcoxon signed rank test results comparing STRUCTURE results from the real data to the hybrid swarm model and the mechanical mixing model. (XLSX)