Robert Baines's research while affiliated with University College London and other places

... To examine the role of Vwa1 in development, a strain from a genome-wide screen that was reported to have an insertion at codon-15 of its vwa1 ORF (Gruenheit et al., 2021), was analyzed. This strain, referred to as Vwa1-N2, was sporulation-negative. ...

... Moreover, tenfold more plates must be prepared to analyse tenfold more mutants; hence, it is important to improve the efficiency of selection methods. For selection of REMI and NTG-treated mutants in Dictyostelium, various powerful strategies were applied, including adhesion selection on 10-cm plates, electrotaxis experiments with barcoded microplates, high-throughput growth and development assays that affect lithium and valproic acid, and counterselection with 5-fluoroorotic acid to enrich genes that suppress polyglutamine aggregation 28,[54][55][56] . Because these techniques allow for the screening of numerous cells, they are easily adaptable to CRISPR mutant pools with millions of mutants. ...

... A recently identified PTM form that is directly influenced by CoA levels is CoAlation [21,23,[47][48][49][50]. CoA contains a thiol group that can interact with other thiol groups to form a disulphide or mixed disulphide, such as CoA-cysteine and CoA-glutathione. ...