Richard Barry's research while affiliated with Oxford University Hospitals NHS Trust and other places
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Publications (3)
Many diagnostic antibodies are generated by immunization with whole cells or cell extracts and are shown by screening on tissue sections to label specific cell populations. However, their target molecule then needs to be identified, and this can be technically demanding. Here we describe the use of protein arrays to define the targets of new or unc...
Transmembrane adaptor proteins (of which 7 have been identified so far) are involved in receptor signaling in immune cells. They have only a short extracellular region, with most of the molecule comprising a substantial intracytoplasmic region carrying multiple tyrosine residues that can be phosphorylated by Src- or Syk-family kinases. In this pape...
During the course of raising monoclonal antibodies, reagents are often produced that are not directed against the immunising antigen. These may pass unnoticed unless a screening step based on immunostaining of human tissue is included. Many of these reagents are auto-antibodies, often directed against intracellular targets (e.g. nuclear components)...
Citations
... vi) Hydrogen atom refers to the radioactive tracing of brain activity under particular tests and/or trials (Antoniewicz, Kelleher, & Stephanopoulos, 2011). vii) By 'molecular targets' are meant small molecules, proteins, nuclei acids, cells, tissues, or even parts of an organism ( Kijanka et al., 2009). viii) In such a way, the molecules transport neurotransmitters back into cells and enzymes, the synthesis or metabolism of which can be used as PET radiotracers (Thanos, Wang, & Volkow, 2008). ...
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... NTAL, non-T-cell activation linker [20], or linker for activation of T-cells 2 (LAT2) [21] or for activation of B cells (LAB) [22], is a lipid raft-membrane protein expressed by monocytes, normal B-, plasma-, and NKcells [23] among others. We have shown that NTAL contributes to AKT phosphorylation in leukemic cells and is depleted from the lipid rafts by APLs [17]. ...
