Rena Kasimu’s research while affiliated with Xinjiang Medical University and other places

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Publications (20)


Figure 1. Flow chart of the rat experiments.
Figure 2. Extracts of SDS roots significantly inhibited FeCl 3-induced rat carotid artery thrombosis. (A) HE staining of rat carotid artery thrombosis in each group; (B) comparison of the thrombus weight in each group; (C) comparison of the ratio of thrombosis area in each group. Before the establishment of FeCl 3-induced rat carotid artery thrombosis models, the rats in each group were treated with the indicated solvent extract of SDS roots or CDDP for 20 days, once a day. n = 10, data are presented as the mean ± SD; Significant difference compare to model group *P < 0.05, and compre to sham group Δ P < 0.05.
Figure 5. Effects of SDS root EtOAc extracts on FeCl 3-induced rat plasma levels of PC. (A) and AT-III (B). Before the establishment of the FeCl 3-induced rat carotid artery thrombosis models, rats in each group were treated with different doses of SDS root EtOAc extract or CDDP for 20 days, once a day, n = 10; data are presented as the mean ± SD; *P < 0.05, significant difference compared to the model group; Δ P < 0.05 compared to the sham group.
Different doses of SDS root ESF suppressed FeCl3-induced rat carotid artery thrombosis. (A) HE staining of rat carotid artery thrombosis in each group; (B) comparison of the rat thrombus weight in each group; (C) comparison of the ratio of thrombosis area in each group. Before the establishment of FeCl3-induced rat carotid artery thrombosis models, rats in each group were treated with different doses of SDS root ESF or CDDP for 20 days, once a day, n = 10; data are presented as the mean ± SD; *P < 0.05, significant difference compared to the model group; ΔP < 0.05 compared to the sham group.
Effects of SDS root ESF on FeCl3 induced rat plasma levels of TXB2 and 6-keto-PGF1α. (A) TXB2/6-keto-PGF1α ratio; (B) and vWF (C). Before the establishment of FeCl3-induced rat carotid artery thrombosis models, rats in each group were lavaged with different doses of SDS root EtOAc extract or CDDP for 20 days, once a day. n = 10; data are presented as the mean ± SD; *P < 0.05, significant difference compared to the model group; ΔP < 0.05 compared to the sham group.

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Antithrombotic effects and related mechanisms of Salvia deserta Schang root EtOAc extracts
  • Article
  • Full-text available

December 2018

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249 Reads

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17 Citations

Rena Kasimu

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Xinling Wang

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Xiaomei Wang

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[...]

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Yuming Mu

Salvia deserta Schang (SDS) belongs to the same family as Salvia miltiorrhiza bunge, one of the antithrombotic Chinese herbal medicines. In our study, EtOAc root extracts were analyzed for their effects on adenosine diphosphate (ADP)-induced platelet aggregation in rabbits and FeCl3-induced rat common carotid artery thrombosis as well as on rat blood plasma concentrations of thromboxane B2 (TXB2), 6-keto-prostaglandin F1 alpha (6-keto-PGF1α), antithrombin-III (AT-III), protein C (PC), plasminogen (PLG), plasminogen activator inhibitor (PAI-1), von Willebrand factor (vWF) and tissue-type plasminogen activator (t-PA). EtOAc extracts from SDS roots had significant inhibitory effects on ADP-induced maximum platelet aggregation rate (10.2 ± 2.6 vs control 35.7 ± 5.2; P < 0.05), reduced the FeCl3-induced rat common carotid artery thrombus weight and thrombus area ratio (P < 0.05), significantly decreased plasma TXB2, vWF and PAI-1 levels and increased 6-keto-PGF1α and t-PA levels in a dose dependent manner (all P < 0.05). Thus, the ratio of TXB2/6-keto-PGF1α was significantly decreased (P < 0.05), while the ratio of t-PA/PAI-1 was significantly increased (P < 0.05). In addition, enhanced AT-III and PC activities indicated coagulation inactivation effects of EtOAc SDS root extracts. EtOAc extraction from SDS showed antithrombotic effects, which are likely due to platelet adhesion and aggregation inhibition as well as anticoagulant activities.

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Figure 1. Changes in 24 h urine protein, blood urea nitrogen (BUN), and serum creatinine (Scr) levels. * p < 0.05, ** p < 0.01 compared to the immunoglobulin A nephropathy (IgAN) model group. A: Normal, healthy controls; B: IgAN rat models; C: prednisone acetate group (PAG); D: tripterygium glycoside tablet group; E-G: treatment group that received 0.5, 1, and 2 g/kg periostracum cicadae, respectively. 
Table 1 . IgA fluorescence intensities.
Figure 2. Changes in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), total cholesterol (TCHO), triacylglyceride (TAG), blood urea nitrogen (BUN) and creatinine (CREA) levels. * p < 0.05, ** p < 0.01 compared to the IgAN model group. A: Normal, healthy controls; B: IgAN model group; C: prednisone acetate group; D: tripterygium glycoside tablet group; E-G: treatment group that received 0.5, 1, and 2 g/kg periostracum cicadae, respectively. 
Figure 4. Effects of periostracum cicadae on the serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin 6 (IL-6) in IgAN rats (* p < 0.05, ** p < 0.01). A: Healthy, normal control group; B: IgAN model group; C: prednisone acetate group; D: tripterygium glycoside tablet group; E-G: treatment group that received 0.5, 1, and 2 g/kg periostracum cicadae, respectively. 
Figure 6. Cont. 
Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model

May 2018

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103 Reads

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22 Citations

International Journal of Molecular Sciences

Periostracum cicadae, the cast-off shell of the cicada Cryptotympana pustulata Fabricius, is used in traditional Chinese medicine for its diaphoretic, anticonvulsive, sedative, antipyretic, and antiallergic effects. However, the exact pathogenesis of immunoglobulin A nephropathy (IgAN) remains unclear, thereby hindering investigations to identify novel therapeutic agents. A rat IgAN model was established by administration of bovine serum albumin, lipopolysaccharide, and carbon tetrachloride, which simultaneously established blood stasis and a heat syndrome model. The animals were sacrificed to detect changes in protein levels in urine and blood. Immunofluorescence was performed to assess IgA deposition in the glomeruli. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin 6 (IL-6) levels were measured in bronchoalveolar lavage fluid (BALF) by enzyme-linked immunosorbent assay. Hematology and eosin, periodic acid-Schiff, TUNEL (TdT-mediated dUTP Nick-End Labeling), and immunohistochemical staining were performed to evaluate histopathological changes in kidney tissues. Additionally, target-related proteins were measured by Western blotting. Periostracum cicadae resulted in a reduction in blood and urine protein levels. Serum TNF-α, IL-1β, and IL-6 levels significantly decreased in the periostracum cicadae-treated groups compared to the IgAN group. Furthermore, a reduction in MCP-1 (Monocyte Chemotactic Protein-1), TLR4 ((Toll-Like Receptor 4)), and IgA expression levels and a dose-dependent increase in caspase 3 expression were observed in response to periostracum cicadae treatment. TGF-β1(Transforming Growth Factor-β) levels decreased, whereas that of Fas increased in the kidney tissues of the periostracum cicadae-treated groups. The findings of the present study indicate that periostracum cicadae induces apoptosis and improves kidney inflammation and fibrosis in IgA nephropathy rat models.


Figure 1. Changes in 24-h urine protein, blood urea nitrogen (BUN), and serum creatinine (Scr) levels. 
Figure 2. Changes in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total 
Figure 7. Effects of periostracum cicadae on FAS levels in the kidney. (a) representative 
Figure 8. TLR4, MCP-1, IgA, and caspase-3 expression in rat kidney tissues. (a) TLR4, IgA, MCP-1, 
Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in IgA Nephropathy Rat Model

May 2018

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53 Reads

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5 Citations

Periostracum cicadae, the cast-off shell of the cicada Cryptotympana pustulata Fabricius, is used in traditional Chinese medicine for its diaphoretic, anticonvulsive, sedative, antipyretic, and antiallergic effects. However, the exact pathogenesis of immunoglobulin A nephropathy (IgAN) remains unclear, thereby hindering investigations to identify novel therapeutic agents. A rat IgAN model was established by administration of bovine serum albumin, lipopolysaccharide, and carbon tetrachloride, which simultaneously established blood stasis and a heat syndrome model. The animals were sacrificed to detect changes in protein levels in urine and blood. Immunofluorescence was performed to assess IgA deposition in the glomeruli. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin 6 (IL-6) levels were measured in bronchoalveolar lavage fluid (BALF) by enzyme-linked immunosorbent assay. Hematology and eosin, periodic acid-Schiff, TUNEL, and immunohistochemical staining were performed to evaluate histopathological changes in kidney tissues. Additionally, target-related proteins were measured by western blotting. Periostracum cicadae resulted in a reduction in blood and urine protein levels. Serum TNF-α, IL-1β, and IL-6 levels significantly decreased in the periostracum cicadae-treated groups compared to the IgAN group. Furthermore, a reduction in MCP-1, TLR4, and IgA expression levels and a dose- dependent increase in caspace-3 expression were observed in response to periostracum cicadae treatment. TGF-β1 levels decreased, whereas that of Fas increased in the kidney tissues of the periostracum cicadae-treated groups. The findings of the present study indicate that periostracum cicadae induces apoptosis and improves kidney inflammation and fibrosis in IgA nephropathy rat models.


Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity

July 2017

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18 Reads

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28 Citations

International Journal of Biological Macromolecules

A water-soluble polysaccharide named ESBP2-1 was isolated from Erythronium sibiricum bulb through anion-exchange and size-exclusion chromatography. ESBP2-1 is a neutral polysaccharide fraction with an average molecular weight of 9.4 × 10⁵ Da and is composed of glucose, galactose and arabinose in a ratio of 24.3:1.1:1. Methylation analysis, partial hydrolysis and NMR studies revealed that the backbone of ESBP2-1 primarily consists of repeating →1)-α-D-Glcp-(4→ units where the disaccharide side chains of Arap-(1 → 6)-α-D-Galp-(1→ residue are attached to the O-6 position of Glc. An in vitro assay showed that ESBP2-1 significantly promoted the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cells. In addition, ESBP2-1 stimulated the production of secretory molecules (nitric oxide, TNF-α and IL-1β) of RAW264.7 cells in a dose-dependent manner. These results suggest that ESBP2-1 is a potential immunostimulator.


Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang, China

February 2017

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245 Reads

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21 Citations

BMC Complementary and Alternative Medicine

Background Agastache rugosa is well-known as a common traditional Chinese medicine, which have relieving summer-heat, analgesic and antipyretic effects, have long been used as folkloristic remedy in the treatment of several infectious diseases, anti-inflammatory, and for its antibacterial properties. Considering the lack of available data on the morphology, anatomy and in vitro activity of A. rugosa, the goal of the present study was to carry out the microscopic identification of its aerial parts and in vitro activity research as a contribution to the quality control and reasonable utilization involving A. rugosa. Methods The present study was (a) to describe the microscopic identification with usual light and scanning electron microtechniques of A. rugosa, collected from Xinjiang Region; (b) based on previous research on the essential oil constituents among different parts of A. rugosa from Xinjiang by GC-MS method, to evaluate its antibacterial effect and cell viabilitity assay. ResultsThe microscopic identification of botanical material showed some typical structure. The essential oils from the dried flower (EOF) and leaves (EOL) of A. rugosa were 0.29% and 0.57% (w/w), respectively. The in vitro antibacterial activities showed strong inhibition against S.aureus, E. coli of EOF; strong inhibition against E. coli of EOL. Based GC-MS analysis, the MTT assay showed a dose and time-dependent increase in damage for gastric cancer cell line SGC-7901. Conclusions The results of this work, based on an extensive analytical characterization of the EOF and EOL chemical composition, compared with other origins, showed A. rugosa possessed antibacterial and cytotoxicity properties activities, which need much additional work to open up new biomedical application of these components.


Genome-wide analysis suggests divergent evolution of lipid phosphotases/phosphotransferase genes in plants

July 2016

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25 Reads

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4 Citations

Genes of the LPPT (lipid phosphatase/phosphotransferase) family play important roles in lipid phosphorous transfer and triacylglycerol accumulation in plants. To provide overviews of the plant LPPT family and their overall relationships, here we carried out genome-wide identifications and analyses of plant LPPT family members. A total of 643 putative LPPT genes were identified from 48 sequenced plant genomes, among which 205 genes from 14 plants were chosen for further analyses. Plant LPPT genes belonged to three distinctive groups, namely the LPT (lipid phosphotransfease), LPP (lipid phosphatase), and pLPP (plastidic lipid phosphotransfease) groups. Genes of the LPT group could be further partitioned into three groups, two of which were only identified in terrestrial plants. Genes in the LPP and pLPP groups experienced duplications in early stages of plant evolution. Among 17 Zea mays LPPT genes, divergence of temporal-spatial expression patterns was revealed based on microarray data analysis. Peptide sequences of plant LPPT genes harbored different conserved motifs. A test of Branch Model versus One-ratio Model did not support significant selective pressures acting on different groups of LPPT genes, although quite different nonsynonymous evolutionary rates and selective pressures were observed. The complete picture of the plant LPPT family provided here should facilitate further investigations of plant LPPT genes and offer a better understanding of lipid biosynthesis in plants.


New triterpenoids from the latex of Euphorbia resinifera Berg

November 2015

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154 Reads

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36 Citations

Fitoterapia

Nine novel compounds, six euphane triterpenes, Euphorol A-D (1-4), H (8) and I (9), and three tirucallane triterpenes, Euphorol E-G (5-7) including four nortriterpenes, together with seven known compounds (10-16) have been isolated from the methanol extraction of Euphorbium. Their structures were established on the basis of extensive analyses of their HR-ESI-MS, UV, IR, 1D and 2D NMR methods. A putative biogenetic relationship to these compounds was proposed. The cytotoxicity of all these isolates against MCF-7, U937 and C6 cancer cell lines was evaluated. Compounds 1-3, 10, 11 and 13-16 exhibited moderate cytotoxic activities.


Optimised extraction of Erythronium sibiricum bulb polysaccharides and evaluation of their bioactivities

October 2015

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20 Reads

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20 Citations

International Journal of Biological Macromolecules

In this study, the extraction of Erythronium sibiricum bulb polysaccharides (ESBP) through hot water decoction was optimised using response surface methodology (RSM) and a three-level, four-factor Box-Behnken design. The optimum extraction conditions were as follows: extraction time of 4.28h, extraction temperature of 90°C, ratio of liquid to raw material of 37mL/g and extraction cycle number of three. The experimental yield (37.25%±0.17%) agreed with the predicted value of the RSM model (37.465%). Preliminary ESBP characterisation was conducted through physicochemical analysis. Biological activity test results showed that ESBP exhibited antioxidant activities and excellent anti-inflammatory and analgesic activities, indicating its potential as an anti-inflammatory and analgesic agent.


Anti-platelet aggregation activities of different fractions in leaves of Apocynum venetum L

March 2015

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48 Reads

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15 Citations

Journal of Ethnopharmacology

Ethnopharmacological relevance Apocynum venetum is widely used in Uygur and traditional Chinese medicine. Modern pharmaceutical studies have shown that leaves of A. venetum. have effects of liver protection, antidepressant and regulation of blood pressure. However, it is unclear that which components have pharmacological activities. The aim was to study chemical constituents of A. venetum. and its anti-platelet aggregation activity. Nephelometery was applied to evaluate anti-platelet aggregation activity of multi-components of A venetum. Systematic separation components were characterized by HPLC analysis method, and in vitro screening active components by anti-platelet aggregation study. Ethyl acetate fraction (L-III) and L-III-4 have better anti-platelet aggregation activity than other fractions. The results indicated that isoquercitrin, hyperoside and other flavonoids have anti-platelet aggregation activity in A. venetum. Our studies provide basis on the endeavors of screening chemicals with that anti-platelet aggregation activity in leaves of A. venetum. Copyright © 2015. Published by Elsevier Ireland Ltd.


Citations (12)


... and are distributed in China [3]. The components mainly include diterpenoids and phenolic acids [1,4] and contain ingredients with medicinal values such as antithrombotic [5], antioxidant [6][7][8], anti-tumor [1,7,8], and antiviral [1,9]. The famous traditional Chinese medicine Salvia miltiorrhiza is a plant of the Salvia spp. in the Lamiaceae family, with the effects of promoting blood circulation, removing blood stasis [4], and reducing swelling and pain [7], which is widely applied to treat cardiovascular diseases in clinical practice [1,8,10]. ...

Reference:

Content Determination and Chemical Clustering Analysis of Tanshinone and Salvianolic Acid in Salvia spp
Antithrombotic effects and related mechanisms of Salvia deserta Schang root EtOAc extracts

... Periostracum cicadae (PC), the cast-off shell of the Cryptotympana pustulata Fabricius belonging to the cicadidae, is an animal-based traditional Chinese medicine in Korea and China [10]. PC possess many interesting pharmacological and physiological activities, such as diaphoretic, anti-convulsive, sedative, anti-pyretic, and anti-allergic effects [11,12]. PC is originally described in Ming-I-Pieh-Lu (an ancient Chinese medical book dating to the Han Dynasty), which is used to treat convulsions, the nocturnal crying of children, delirium, and feverish chills [13]. ...

Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model

International Journal of Molecular Sciences

... The surface exhibits irregularities with rough pores and a multibranched aggregation structure. The intertwining and gathering of branching chains may be attributed to the methods used for preparing purified polysaccharides (Kasimu et al., 2017). It can be inferred that there is a strong interaction force between PSP molecules, leading to their tight arrangement in space and the formation of a dense network structure on the surface (Shi, 2016). ...

Water-soluble polysaccharide from Erythronium sibiricum bulb: Structural characterisation and immunomodulating activity
  • Citing Article
  • July 2017

International Journal of Biological Macromolecules

... These compounds have protective effects on human health, especially related to their antioxidant activity (Dwivedi et al., 2016), their anti-inflammatory, antibacterial, antiviral, anti-skin ageing, anti-cancer properties, and to osteoporosis, insulin sensitivity, obesity, and cardiovascular disease (Matsumura et al., 2023;Mutha et al., 2021). It is a plant source containing both bioactive compounds (Desta et al., 2016) used to treat anxiety, cholera, infections, nausea, and gastrointestinal problems (Cao et al., 2017;Gong et al., 2017), and antimicrobial and antifungal agents (Desta et al., 2016) (Table 1). It also possesses HIV integrase inhibitory activity and is a potential drug candidate against COVID-19 (Adhikari et al., 2021). ...

Microscopic identification and in vitro activity of Agastache rugosa (Fisch. et Mey) from Xinjiang, China

BMC Complementary and Alternative Medicine

... In this case, a sustained expression of the WT FXN through gene therapy might augment restoration of neurodegenerative symptoms back to normal. Since there is a set of previous reports [42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61] defining a role for phosphatases such as Protein phosphatase 2A (PP2A) as a negative regulator of protein kinase function and activation, future studies should warrant the potential consequences of PP2A [50] or similar phosphatase function in the absence or presence of mutations in the 129 genes encoding protein kinases investigated in this study. ...

Genome-wide analysis suggests divergent evolution of lipid phosphotases/phosphotransferase genes in plants
  • Citing Article
  • July 2016

... Este látex contiene triterpenoides, diterpenoides, flavonoides, ácidos fenólicos y taninos entre otros principios activos. Recientemente, se ha comprobado que el látex tiene propiedades antitumorales, antivirales, antidiarrea, antihelmíntico, cicatrizantes y como relajante vascular (Wang et al., 2016). ...

New triterpenoids from the latex of Euphorbia resinifera Berg
  • Citing Article
  • November 2015

Fitoterapia

... Our findings demonstrated a positive correlation between the number of extraction cycles and the ARP yield, with a plateau observed beyond three cycles, as illustrated in Figure 1d. This trend is in agreement with findings from other studies [27]. Considering the balance between extraction efficiency and resource optimization, a threecycle extraction protocol was selected for this research. ...

Optimised extraction of Erythronium sibiricum bulb polysaccharides and evaluation of their bioactivities
  • Citing Article
  • October 2015

International Journal of Biological Macromolecules

... Our interest in enrofloxacin and related fluoroquinolones is focused on their potential as multidentate/bridging ligands in coordination chemistry. The cadmium, lead, copper, zinc and cobalt complexes of the enrofloxacin anion have been reported [2][3][4][5] . In the title compound, [Mn(C 19 (2) ...

Crystal structure of di-aqua-bis-[1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluor-4-oxo-1,4-d ihydrochinolin-3-carboxylic acid]zinc(II)bibenzene-1,4-dicarboxylate octahydrate, [Zn(C19H22N3FO3)(2)(H2O)(2)]center dot 2(C8H5O4)center dot 8(H2O), C54H74F2N6O24Zn

Zeitschrift für Kristallographie. New crystal structures

... Qug, as the active compound from Nelumbinis Folium, inhibits lipid peroxidation, ameliorates lipid accumulation in the liver and prevents atherosclerosis (Ohara et al., 2013;Queiroz et al., 2017;Wang et al., 2015). Hyp is one of the major constituents in Nelumbinis Folium and Crataegi Fructus, which has been proved to have anti-oxidative stress, anti-hyperlipidemia and anti-platelet aggregation effects (Kasimu et al., 2015;Park et al., 2016;Zhang et al., 2017). In addition, IQ is also distributed in Nelumbinis Folium and PO,2. ...

Anti-platelet aggregation activities of different fractions in leaves of Apocynum venetum L
  • Citing Article
  • March 2015

Journal of Ethnopharmacology

... Paeonol (83) and its derivatives generally display three absorption maxima bands at 225-230, 270-280 and 300-320 nm, respectively [121]. In order to determine various compounds at its peak absorbance wavelength, UV switch methods simultaneously monitoring multiple wavelength were used [122,123]. For example, Ding Yan et al. developed a HPLC method to determine the content of eight pharmacological compounds, namely, gallic acid (97), paeoniflorin (12), galloylpaeoniflorin (15), benzoic acid (104), quercetin (63), benzoylpaeoniflorin (17), paeoniflorigenone (1), and paeonol (83) [124]. ...

A Novel Method HPLC-DAD Analysis of the Contentsof Moutan Cortexand Paeoniae Radix Alba with Similar Constituents-Monoterpene Glycosides in Guizhi Fuling Wan

Molecules