Raúl Arredondo-Peter's research while affiliated with Universidad Autónoma del Estado de Morelos and other places

Publications (59)

Article
Full-text available
Non-symbiotic hemoglobins (nsHbs) are widely distributed in land plants, including rice. These proteins are classified into type 1 (nsHbs-1) and type 2. The O 2-affinity of nsHbs-1 is very high mostly because of an extremely low O 2-dissociation rate constant resulting in that nsHbs-1 apparently do not release O 2 after oxygenation. Thus, it is pos...
Article
Full-text available
Hemoglobin (Hb) is a heme-containing protein found in the red blood cells of vertebrates. For many years, the only known Hb-like molecule in plants was leghemoglobin (Lb). The discovery that other Hb-like proteins existed in plants led to the term "nonsymbiotic Hbs (nsHbs)" to differentiate them from the Lbs. While this terminology was adequate in...
Article
Full-text available
Non-symbiotic hemoglobins (nsHbs) are widely distributed in land plants, including rice. These proteins are classified into type 1 (nsHbs-1) and type 2. The O 2-affinity of nsHbs-1 is very high mostly because of an extremely low O 2-dissociation rate constant resulting in that nsHbs-1 apparently do not release O 2 after oxygenation. Thus, it is pos...
Article
Full-text available
Globins (Glbs) are proteins widely distributed in organisms. Three evolutionary families have been identified in Glbs: the M, S and T Glb families. The M Glbs include flavohemoglobins (fHbs) and single-domain Glbs (SDgbs); the S Glbs include globin-coupled sensors (GCSs), protoglobins and sensor single domain globins, and the T Glbs include truncat...
Article
Full-text available
Hemoglobins (Hbs) corresponding to non-symbiotic (nsHb) and truncated (tHb) Hbs have been identified in rice (Oryza). This review discusses the major findings from the current studies on rice Hbs. At the molecular level, a family of the nshb genes, consisting of hb1, hb2, hb3, hb4 and hb5, and a single copy of the thb gene exist in Oryza sativa var...
Article
Full-text available
Hemoglobins (Hbs) corresponding to non-symbiotic (nsHb) and truncated (tHb) Hbs have been identified in rice ( Oryza). This review discusses the major findings from the current studies on rice Hbs. At the molecular level, a family of the nshb genes, consisting of hb1, hb2, hb3, hb4 and hb5, and a single copy of the thb gene exist in Oryza sativa va...
Article
Hemoglobins (Hbs) corresponding to non-symbiotic (nsHb) and truncated (tHb) Hbs have been identified in rice (Oryza). This review discusses the major findings from the current studies on rice Hbs. At the molecular level, a family of the nshb genes, consisting of hb1, hb2, hb3, hb4 and hb5, and a single copy of the thb gene exist in Oryza sativa var...
Article
Full-text available
Non-symbiotic (nsHb) and truncated (tHb) hemoglobins (Hbs) have been detected in a variety of land plants. The evolution of land plant nsHbs and tHbs at the protein level is well documented; however, little is known about the evolution of genes coding for these proteins. For example, the variability of the land plant nshb and thb genes is not known...
Article
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Ferrous oxygenated hemoglobins (Hb(2+)O2) autoxidize to ferric Hb(3+), but Hb(3+) is reduced to Hb(2+) by enzymatic and non-enzymatic mechanisms. We characterized the interaction between the soybean ferric leghemoglobin reductase 2 (FLbR2) and ferric rice non-symbiotic Hb1 (Hb1(3+)). Spectroscopic analysis showed that FLbR2 reduces Hb1(3+). Analysi...
Article
Full-text available
Non-symbiotic hemoglobins (nsHbs) are O(2)-binding proteins widely distributed in land plants, including primitive bryophytes. Little is known about the properties of bryophyte nsHbs. Here, we report the spectroscopic characterization of two moss recombinant nsHbs, CerpurnsHb of Ceratodon purpureus and PhypatnsHb of Physcomitrella patens. Spectra s...
Article
This review discusses the evolution of land plant hemoglobins within the broader context of eukaryote hemoglobins and the three families of bacterial globins. Most eukaryote hemoglobins, including metazoan globins and the symbiotic and non-symbiotic plant hemoglobins, are homologous to the bacterial 3/3-fold flavohemoglobins. The remaining plant he...
Article
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Two types of non-symbiotic hemoglobins (nsHbs) have been detected in angiosperms: nsHb-1 and nsHb-2. The origin of nshb-1 and nshb-2 prior to the monocot-dicot divergence was a major event in the evolution of land plant nsHbs. Because nshb-2 has not been detected in monocots, apparently only nshb-1 exists in monocots. Here, we report the phylogenet...
Article
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It has been well established that land plant non-symbiotic hemoglobin (nshb), symbiotic hemoglobin (shb) and leghemoglobin (lb) genes are interrupted by 3 introns (IVS-I, IVS-II and IVS-III). Here, we report the detection, PCR-amplification and DNA sequencing of a 4 intron-containing nshb (nshb2) gene from the Physcomitrella patens DNA. Sequence an...
Article
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Two phylogenetically unrelated superoxide dismutase (SOD) families, i.e., CuZnSOD (copper and zinc SOD) and FeMn-CamSOD (iron, manganese, or cambialistic SOD), eliminate superoxide radicals in different locations within the plant cell. CuZnSOD are located within the cytosol and plastids, while the second family of SOD, which are considered to be of...
Article
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Rice (Oryza sativa) contains five copies of the non-symbiotic hemoglobin (hb) gene, namely hb1 to hb5. Previous analysis by RT-PCR revealed that rice hb1 expresses in roots and leaves and hb2 expresses in leaves. However, it is not known whether or not hb1 and hb2 express in rice embryonic organs. Here, we report the expression of hb1 and hb2 genes...
Article
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Land plants and algae are now represented by about 40 genomes. Although most are incomplete, putative globins appear to be present in all the ca. 30 land plant genomes and in all except one algal genomes. The globins have either the canonical 3/3 α-helical fold characteristic of vertebrate myoglobin (Mb) or 2/2 α-helical folds, characteristic of ba...
Article
Three types of hemoglobins (Hbs) exist in land plants: symbiotic Hbs (including to leghemoglobins (Lbs)), non-symbiotic Hbs (nsHbs), and truncated Hbs (tHbs). Although a general phylogeny and evolution for land plant Hbs emerged over the last several years little is known about the evolutionary rate of this group of proteins. This work reports the...
Article
Land plants and algae form a supergroup, the Archaeplastida, believed to be monophyletic. We report the results of an analysis of the phylogeny of putative globins in the currently available genomes to bacterial and other eukaryote hemoglobins (Hbs). Archaeplastida genomes have 3/3 and 2/2 Hbs, with the land plant genomes having group 2 2/2 Hbs, ex...
Article
In plants, it has been proposed that hexacoordinate (class 1) non-symbiotic Hbs (nsHb-1) function in vivo as peroxidases. However, little is known about peroxidase activity of nsHb-1. We evaluated the peroxidase activity of rice recombinant Hb1 (a nsHb-1) by using the guaiacol/H2O2 system at pH 6.0 and compared it to that from horseradish peroxidas...
Article
Full-text available
Nonsymbiotic hemoglobins (nsHbs) are widespread in plants including bryophytes. Bryophytes (such as mosses) are among the oldest land plants, thus an analysis of a bryophyte nsHb is of interest from an evolutionary perspective. However, very little is known about bryophyte nsHbs. Here, we report the cloning and characterization of an nshb gene (cer...
Article
Nonsymbiotic hemoglobins (nsHbs) and leghemoglobins (Lbs) are plant proteins that can reversibly bind O(2) and other ligands. The nsHbs are hexacoordinate and appear to modulate cellular concentrations of NO and maintain energy levels under hypoxic conditions. The Lbs are pentacoordinate and facilitate the diffusion of O(2) to symbiotic bacteroids...
Article
This work reports the analysis of an additional hemoglobin (hb) gene copy, hb5, in the genome of rice. The amino acid sequence of Hb5 differs from the previously determined rice Hbs 1-4 in missing 11 residues in helix E. Transcripts of hb5 were found to be ubiquitous in rice organs, and hormone- and stress-response promoters exist upstream of the r...
Article
Ferric leghemoglobin reductase (FLbR) is able to reduce ferric leghemoglobin (Lb3+) to ferrous (Lb2+) form. This reaction makes Lb functional in performing its role since only reduced hemoglobins bind O2. FLbR contains FAD as prosthetic group to perform its activity. FLbR-1 and FLbR-2 were isolated from soybean root nodules and it has been postulat...
Article
Amino acid sequences for more than 60 plant hemoglobins (Hbs) are deposited in databases, but the tertiary structure of only 4 plant Hbs have been reported; thus, the gap between the reported sequences and structures of plant Hbs is large. Elucidating the structure of plant Hbs is essential to fully understanding the function of these proteins in p...
Article
This review describes contributions to the study of plant hemoglobins (Hbs) from a historical perspective with emphasis on non-symbiotic Hbs (nsHbs). Plant Hbs were first identified in soybean root nodules, are known as leghemoglobins (Lbs) and have been characterized in detail. It is widely accepted that a function of Lbs in nodules is to facilita...
Article
Nonsymbiotic hemoglobins (nsHb) are proteins widely distributed in land plants, however their function in plant organs is still not known. Rice nsHbs have been characterized in detail and represent a model to study plant nsHbs. Rice recombinant Hb1 (rHb1) has a very high affinity for O2 (1,800 μM-1 s-1), because the dissociation constant is extreme...
Article
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Influence of expressed leghemoglobin on stability of Escherichia coli cells to reactive oxygen and nitrogen species – tert-butyl hydroperoxide and S-nitrosoglutathione was studied. We determined both action of these compounds on cells with different level of leghemoglobin expression and peroxidase activity of the cells. It was shown that priority o...
Data
Fig. 1. Alignment of selected globin sequences. Fig. 2. Alignment of putative globins from Caenorhabditis briggsae and C. elegans. Fig. 3. Alignment of 175 representative globin sequences used in the construction of the Bayesian phylogenetic tree shown in Fig. 5.
Data
Table 1. List of genomes from the three kingdoms of life used in the present study. Table 2. Identified and putative globins in archaeal genomes. Table 3. Phylogenomic distribution of identified and putative globins in bacteria. Table 4. Identified and putative globins in eukaryote genomes. Table 5. The putative globin orthologs of Caenorhabditis b...
Article
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Globins occur in all three kingdoms of life: they can be classified into single-domain globins and chimeric globins. The latter comprise the flavohemoglobins with a C-terminal FAD-binding domain and the gene-regulating globin coupled sensors, with variable C-terminal domains. The single-domain globins encompass sequences related to chimeric globins...
Article
Computational methods, such as the ADM (average distance map) method, have been developed to predict folding of homologous proteins. In this work we used the ADM method to predict the folding pathway and kinetics among selected plant nonsymbiotic (nsHb), symbiotic (Lb), and truncated (tHb) hemoglobins (Hbs). Results predicted that (1) folding of pl...
Article
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Although most globins, including the N-terminal domains within chimeric proteins such as flavohemoglobins and globin-coupled sensors, exhibit a 3/3 helical sandwich structure, many bacterial, plant, and ciliate globins have a 2/2 helical sandwich structure. We carried out a comprehensive survey of globins in the genomes from the three kingdoms of l...
Article
The tertiary structure of a maize (Zea mays ssp. mays) non-symbiotic hemoglobin (Hbm) was modeled using computer tools and the known tertiary structure of rice Hb1 as a template. This method was tested by predicting the tertiary structure of soybean leghemoglobin a (Lba) using rice Hb1 as a template. The tertiary structures of the predicted and nat...
Article
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Using in silico methods, several putative phytohormone-responsive cis-elements in the Oryza sativa non-symbiotic haemoglobin (NSHB) 1-4 and Arabidopsis thaliana NSHB1-2 promoters have been identified. An OsNSHB2 promoter::GUS reporter gene fusion shows tissue-specific expression in A. thaliana. GUS expression was observed in roots, the vasculature...
Article
Hemoglobins (Hbs) have been characterized from a wide variety of eubacteria, but not from nitrogen-fixing rhizobia. Our search for Hb-like sequences in the Sinorhizobium meliloti genome revealed that a gene coding for a flavohemoglobin (fHb) exists in S. meliloti (SmfHb). Computer analysis showed that SmfHb and Alcaligenes eutrophus fHb are highly...
Article
A hemoglobin (hb) gene family was identified in rice, consisting of four functional hb copies located in BAC inserts OSM11719 and OSM11676, which contain the gene clusters hb1/hb3/hb4 and hb2, respectively. Sequence comparison with databases revealed that a number of potential promoters exist upstream from the hb genes suggesting that these genes a...
Article
Full-text available
Nodule ferric leghemoglobin reductase (FLbR) and leaf dihydrolipoamide reductase (DLDH) belong to the same family of pyridine nucleotide-disulfide oxidoreductases. We report here the cloning, expression, and characterization of a second protein with FLbR activity, FLbR-2, from soybean (Glycine max) nodules. The cDNA is 1,779 bp in length and codes...
Article
Nodule ferric leghemoglobin reductase (FLbR) and leaf dihydrolipoamide reductase (DLDH) belong to the same family of pyridine nucleotide-disulfide oxidoreductases. We report here the cloning, expression, and characterization of a second protein with FLbR activity, FLbR-2, from soybean (Glycine max) nodules. The cDNA is 1,779 bp in length and codes...
Article
With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and...
Article
In rice (Oryza sativa var. Jackson) at least three copies of hemoglobin (hb) gene exist. Rice hb1 and hb2 genes are differentially expressed in roots and leaves from mature plants. We used polyclonal antibodies raised to recombinant rice Hb1 and Western blotting to analyze the synthesis of Hbs in rice plants growing under normal or stress condition...
Article
Nonsymbiotic hemoglobins (ns-Hbs) previously have been found in monocots and dicots; however, very little is known about the tissue and cell type localization as well as the physiological function(s) of these oxygen-binding proteins. We report the immunodetection and immunolocalization of ns-Hbs in rice (Oryza sativa L.) by Western blotting and in...
Article
Nonsymbiotic hemoglobins (nsHbs) form a new class of plant proteins that is distinct genetically and structurally from leghemoglobins. They are found ubiquitously in plants and are expressed in low concentrations in a variety of tissues including roots and leaves. Their function involves a biochemical response to growth under limited O(2) condition...
Article
Ferric leghemoglobin reductase (FLbR), an enzyme reducing ferric leghemoglobin (Lb) to ferrous Lb, was purified from cowpea (Vigna unguiculata) root nodules by sequential chromatography on hydroxylapatite followed by Mono-Q HR5/5 FPLC and Sephacryl S-200 gel filtration. The purified cowpea FLbR had a specific activity of 216 nmol Lb2+O2 formed min−...
Article
Oxygen concentration is an environmental signal that regulates nitrogen fixation in the Rhizobium-legume symbiosis, We investigated the effect of the heterologous expression of Vitreoscilla sp, hemoglobin (VHb), which is an oxygen-binding protein, in Rhizobium etli, The vhb gene and its native promoter were subcloned in the plasmid pMR4 and transfo...
Article
Hbs are ubiquitous proteins in most organisms, including bacteria, protozoans, fungi, plants, and animals (Vinogradov et al., 1993; Bolognesi et al., 1997). The known and predicted roles of Hbs in each organism encompass functions that include the reversible binding of gaseous ligands and the ability to bind other cellular molecules.
Article
Full-text available
Although nonsymbiotic hemoglobins (Hbs) are found in different tissues of dicots and monocots, very little is known about hb genes in monocots and the function of Hbs in nonsymbiotic tissues. We report the cloning and analysis of two rice (Oryza sativa L.) hb genes, hb1 and hb2, that code for plant Hbs. Rice hb1 and hb2 genes contain four exons and...
Article
Full-text available
Cowpea (Vigna unguiculata) nodules contain three leghemoglobins (LbI, LbII, and LbIII) that are encoded by at least two genes. We have cloned and sequenced the gene that encodes for LbII (lbII), the most abundant Lb in cowpea nodules, using total DNA as the template for PCR. Primers were designed using the sequence of the soybean lbc gene. The lbII...
Article
The cDNA for soybean leghemoglobin a (Lba) was cloned from a root nodule cDNA library and expressed in Escherichia coli. The crystal structure of the ferric acetate complex of recombinant wild-type Lba was determined at a resolution of 2.2 Å. Rate constants for O2, CO and NO binding to recombinant Lba are identical with those of native soybean Lba....
Article
Phage DNA isolation from genomic and cDNA libraries is time-consuming and cumbersome. Our work reports on the use of a pair of λ gt11 primers to amplify by PCR any insert DNA that is cloned into theEco RI restriction site. Using crude phage pools and direct phage plaques from a corn root cDNA library, we amplified and cloned DNA fragments. The amou...
Article
Several rhizobial strains were isolated from Phaseolus coccineus root nodules and were determined to be Rhizobium leguminosarum biovar phaseoli strains after reinfection of the same host plant. These strains were characterized by cultural procedures (growth on different carbon sources and intrinsic antibiotic resistance) and electrophoretic procedu...
Article
This work describes the gathering of the reported bacterial cytochrome oxidase subunit II sequences and the construction of the corresponding consensus sequence (COXII CS). Such sequence was analyzed by computer procedures and a model for the COXII CS was elaborated. The main features of the COXII CS are fully described and the several further appl...
Article
From the study of plant hemoglobin protein and gene sequences, a consensus sequence was constructed and analysed by computer methods; analysis comprised the amino acid composition, hydropathy profile, and match degree with other plant and non-plant hemoglobin sequences. Resulting consensus sequence shows the main features of hemoglobin genes and pr...
Article
Full-text available
A gene coding for the cowpea (Vigna unguiculata L) leghemoglobin II (LbII) was previously cloned and sequenced. This work concerns the gene and expression analysis of cowpea LbII. Southern blot analysis using the cowpea LbII gene as probe showed that at least two copies of the leghemoglobin gene exist in cowpea. A gene coding for the cowpea leghemo...

Citations

... We herein analyzed the ICE-harboring strains of V. parahaemolyticus JHY20 isolated from shrimps in Xuzhou, Jiangsu, China (Song et al., 2013). Our findings pertaining to the DLDH protein were consistent with those previously reported in the literature (Park et al., 2004;Ono et al., 2006;Salomon et al., 2013); further, consistent with the findings of Jackman et al. (1990), Moran et al. (2002), McMillan et al. (2005), Jiang et al. (2006), and Wu et al. (2008), we report that DLDH is a secreted protein. We found that it is involved in nine signaling pathways, such as citrate cycle, glycolysis, and gluconeogenesis, and considering the information pertaining to its physical and biochemical characterization, we predicted that DLDH plays a key role in metabolic pathways and cytotoxicity. ...
... In a scan of the Rice Genome Annotation Database, the five class 1 rice Pgbs showed organ-specific expression. 35 OsPgbs were preferentially localized in the ovary, embryo, roots, reproductive stems and in response to stress. However, within the reproductive tissue, OsPgb1.5 expression could only be found in the lemma, while OsPgb1.3 was expressed in the palea. ...
... However, numerous other hemebinding globin families have been identified and are found to display distinct functions [2,5,17,18]. For example, several plant globins, including symbiotic leghemoglobin, present in nitrogenfixing root nodules of leguminous plants [19], and non-symbiotic phytoglobins [20][21][22], have functions ranging from oxygen buffering [23] and NO scavenging [24] to facilitating oxygen supply to developing tissues [25,26]. Aerotactic globin-coupled sensors, such as HemAT on the other hand, represent bacterial and archaeal globins that sense the level of diatomic oxygen in the cellular environment [27]. ...
... 1−3 Apart from the well-characterized symbiotic hemoglobins, plant genomes are endowed with multiple nonsymbiotic hemoglobins (nsHbs), the significance of which can be anticipated by their ubiquitous occurrence in almost all land plants. 4,5 The nsHbs are further categorized into three separate classes: class I (nsHb1), class II (nsHb2), and class III or truncated hemoglobins (trHbs). 6 Members of each class bear unique kinetic and structural fingerprints, suggestive of their distinct physiological role. ...
... Generally, phytoglobins are categorized into symbiotic, non-symbiotic and truncated phytoglobins. Symbiotic phytoglobins are localized in the root nodules of nitrogen fixing plants whereas non-symbiotic and truncated phytoglobins are localized in the diverse organs throughout the plants [25,26]. On the basis of oxygen affinity and sequence similarity,nonsymbiotic phytoglobins can be further divided in to type 1 and type 2phytoglobins [27]. ...
... The results obtained are therefore consistent with a monomer in solution; no dimers or larger oligomers are present. Biomolecules 2020, 10, x 7 of 18 An additional estimate of the size of AHb2 in native conditions was obtained by analyzing the electrophoretic mobility (Rf) of AHb2 and comparing it with that of standard proteins with known molecular masses at various polyacrylamide concentrations (8,9,10 and 12%) in native-PAGE ( Figure 2C-E). Only one band was observed for AHb2 at either low (5 μM) or high protein concentrations (25 μM) which corresponds approximately to 18 kDa, a value that is fully consistent with a monomeric species. ...
... Plant Pgbs are known to be differentially expressed under various stresses, such as, hypoxia, nutrient deprivation, osmotic, cold, oxidative, and nitro-sative stress (Arredondo-Peter, Moran & Sarath, 2014;Mira et al., 2016;Mira, El-Khateeb, Sayed Ahmed, Hill, & Stasolla, 2017). The recent reverse genetic approaches on Pgbs in Arabidopsis thaliana have emphasized their paramount role during plant growth and development by demonstrating that at least one functional Pgb gene is necessary for survival of young seedlings (Hebelstrup, Shah, & Igamberdiev, 2013;Hill, 2012). ...
... Evaluation of the rate of divergence of selected land plant Hbs revealed that evolutionary rates slowed down previous to the origin of magnoliophyta and that the rate of divergence was slower in rice Hb1 than in rice tHb 150 . This observation suggested that rice Hb1 (and conceivably other rice nsHbs) evolved under the effect of the stabilizing selection. ...
... The function of this latter class is unknown, although they can also be involved in NO scavenging at high oxygen tensions (reviewed in . Barley does not possess Pgb2; its genome contains Pgb1 and Pgb3 encoding correspondingly the class 1 and truncated (class 3) phytoglobins (Rodríguez-Alonso and Arredondo-Peter, 2013). ...
... The protein was not detectable in very young nodules (25-d-old plants) but was relatively abundant in mature nodules (46-d-old plants) and in senescent nodules (82-d-old plants). In the latter plants, two nodule populations could be distinguished by their color ("red-brown" and "green"), which reflected increased leghemoglobin (Lb) degradation (Arredondo Peter et al., 1997). The VuFeSOD content of green nodules was clearly greater than that of red-brown nodules. ...