Raquel S. Peixoto's research while affiliated with King Abdullah University of Science and Technology and other places
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Publications (65)
Managing marine nonindigenous species (mNIS) is challenging, because marine environments are highly connected, allowing the dispersal of species across large spatial scales, including geopolitical borders. Cross-border inconsistencies in biosecurity management can promote the spread of mNIS across geopolitical borders, and incursions often go unnot...
Here we report the oil degradation genetic potential of six oil-degrading bacteria (ODB), previously used as a bioremediation consortium, isolated from the hydrocoral Millepora alcicornis and seawater. The strains were identified as Halomonas sp. (LC_1), Cobetia sp. (LC_6), Pseudoalteromonas shioyasakiensis (LC_2), Halopseudomonas aestusnigri (LC_3...
Climate change is turning formerly pristine ecosystems into ever-changing states, causing major disturbance and biodiversity loss. Such impacted marine ecosystems and organisms exhibit clear microbiome shifts that alter their function. Microbiome-targeted interventions appear as feasible tools to support organismal and ecosystem resilience and reco...
More than 20,000 species of prokaryotes (less than 1% of the estimated number of Earth’s microbial species) have been described thus far. However, the vast majority of microbes that inhabit extreme environments remain uncultured and this group is termed “microbial dark matter.” Little is known regarding the ecological functions and biotechnological...
Sponges thrive in the deep, dark and nutrient-depleted ocean and may rely on microbial symbionts for carbon acquisition and energy generation. However, these symbiotic relationships remain largely unexplored. In this study, we analyze the microbiome of deep-sea sponges and show that ammonia-oxidizing archaea (AOA) of the family Nitrosopumilaceae ma...
Within microeukaryotes, genetic variation and functional variation sometimes accumulate more quickly than morphological differences. To understand the evolutionary history and ecology of such lineages, it is key to examine diversity at multiple levels of organization. In the dinoflagellate family Symbiodiniaceae, which can form endosymbioses with c...
Calcifying organisms are considered as threatened by ocean acidification, because of their calcium carbonate skeleton. This study investigated if a calcareous sponge could synthesize its skeleton (i.e. spicules) under ocean-acidification conditions. Sponge cell aggregates that have the potential to develop into a functional sponge, called primmorph...
Mycobacteria are ubiquitous microorganisms and may be found in many different environments, either associated or not to diseases. Culture-independent molecular techniques brought new perspectives for research regarding soil microorganisms, which is relevant to microbiologists and industry. Therefore, new research is necessary to broaden our knowled...
The symbiotic relationship between corals and symbiodiniaceans can favor reef formation, but is easily rupted when these organisms are exposed to thermal anomalies. Here, we assessed the ITS2 rDNA phylotype diversity of dominant Symbiodiniaceae lineages associated with the hydrocoral Millepora alcicornis and investigated host–symbiont distribution...
Global biodiversity loss and mass extinction of species are two of the most critical environmental issues the world is currently facing, resulting in the disruption of various ecosystems central to environmental functions and human health. Microbiome-targeted interventions, such as probiotics and microbiome transplants, are emerging as potential op...
Within microeukaryotes, genetic and functional variation sometimes accumulate more quickly than morphological differences. To understand the evolutionary history and ecology of such lineages, it is key to examine diversity at multiple levels of organization. In the dinoflagellate family Symbiodiniaceae, which can form endosymbioses with cnidarians...
Merten’s carpet sea anemone, Stichodactyla mertensii Brandt, 1835, is the largest known sea anemone species in the world, regularly exceeding one meter in oral disc diameter. A tropical species from the Indo-Pacific, S. mertensii drapes prominently over coral reef substrates and is a common host to numerous species of clownfishes and other symbiont...
Micropollutants (MPs) include a wide range of biological disruptors that can be toxic to wildlife and humans at very low concentrations (<1 μg/L). These mainly anthropogenic pollutants have been widely detected in different areas of the planet, including the deep sea, and have impacts on marine life. Because of this potential toxicity, the global d...
Merten’s carpet sea anemone, Stichodactyla mertensii , is the largest known sea anemone species in the world, regularly exceeding one meter in oral disc diameter. A tropical species from the Indo-Pacific, S. mertensii drapes prominently over coral reef substrates and is a common host to numerous species of clownfishes and other symbionts throughout...
Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM for biotechnological purposes has not yet been ad...
Coral research is being ushered into the genomic era. To fully capitalize on the potential discoveries from this genomic revolution, the rapidly increasing number of high-quality genomes requires effective pairing with rigorous taxonomic characterizations of specimens and the contextualization of their ecological relevance. However, to date there i...
this document is the work of a team assembled by the International Coral Reef Society (ICRS). The mission of ICRS is to promote the acquisition and dissemination of scientific knowledge to secure the future of coral reefs, including via relevant policy frameworks and decision-making processes. This document seeks to highlight the urgency of taking...
Bacteria associated with coral hosts are diverse and abundant, with recent studies suggesting involvement of these symbionts in host resilience to anthropogenic stress. Despite their putative importance, the work dedicated to culturing coral-associated bacteria has received little attention. Combining published and unpublished data, here we report...
Host-associated microbiomes contribute in many ways to the homeostasis of the metaorganism. The microbiome's contributions range from helping to provide nutrition and aiding growth, development, and behavior to protecting against pathogens and toxic compounds. Here we summarize the current knowledge of the diversity and importance of the microbiome...
Ocean higher temperature and acidity levels affect its carbonate chemistry, and calcifying organisms that depend on the calcium carbonate saturation state (XCaCO 3) to build their skeleton may be vulnerable to these changes. Despite their important roles in the marine environment , little is known about the vulnerability of calcareous sponges under...
The use of Beneficial Microorganisms for Corals (BMCs) has been proposed recently as a tool for the improvement of coral health, with knowledge in this research topic advancing rapidly. BMCs are defined as consortia of microorganisms that contribute to coral health through mechanisms that include ( a) promoting coral nutrition and growth, ( b) miti...
The use of Beneficial Microorganisms for Corals (BMCs) to increase the resistance of corals to environmental stress has proven to be effective in laboratory trials. Because direct inoculation of BMCs in larger tanks or in the field can be challenging, a delivery mechanism is needed for efficient transmission of the BMC consortium. Packaged delivery...
Enrichment based isolation methods and molecular identification were used to investigate the microbial communities in produced water obtained from Periquito (PQO) and Galo de Campinas (GC) onshore oilfields in Brazil. Produced water was enriched with Postgate B, Postgate C and Baars media and incubated. DNA was extracted, PCR amplified and16S rDNA...
Mangrove ecosystems provide important ecological benefits and ecosystem services, including carbon storage and coastline stabilization, but they also suffer great anthropogenic pressures. Microorganisms associated with mangrove sediments and the rhizosphere play key roles in this ecosystem and make essential contributions to its productivity and ca...
Symbiotic relationships between corals and their associated microorganisms are essential to maintain host homeostasis. Coral‐associated bacteria (CAB) can have different beneficial roles in the coral metaorganism, such as metabolizing essential nutrients for the coral host and protecting the coral from pathogens. Many CAB exert these functions via...
Ethinylestradiol (EE2) is a synthetic estrogen that can cause harmful effects on animals, such as male feminization and infertility. However, the impact of the EE2 contamination on microbial communities and the potential role of bacterial strains as bioremediation agents are underexplored. The aim of this work was to evaluate the impact of EE2 on t...
Have you ever dived into the ocean and seen several colored “rocks” in the water? Those “rocks” might actually be animals called corals! Corals form coral reefs and they are very important, because thousands of marine animals depend on them for survival. Did you know that corals and humans have something in common? Both have what are called mutuali...
Coral reefs are threatened by climate change on a global scale with thermal stress events and mass coral bleaching being widely reported. The reefs off the east coast of Brazil (and other turbid areas) have, however, historically escaped such thermal stress events, with relatively low levels of background coral mortality (5–10%). This has recently...
The significance of symbioses between eukaryotic hosts and microbes extends from the organismal to the ecosystem level and underpins the health of Earth’s most threatened marine ecosystems. Despite rapid growth in research on host-associated microbes, from individual microbial symbionts to host-associated consortia of significantly relevant taxa, l...
In this study, oil spills were simulated in field-based mangrove mesocosms to compare the efficiency of bioremediation strategies and to characterize the presence of the alkB, ndo, assA, and bssA genes and the ecological structures of microbial communities in mangrove sediments at two different depths, (D1) 1–10 cm and (D2) 25–35 cm. The results in...
The significance of mutualisms between eukaryotic hosts and microbes extends from the organismal to the ecosystem level, and mutualistic symbioses underpin the health of Earth’s most threatened marine ecosystems. Despite rapid growth in research on host-associated microbes (microbiomes), very little is known about their interactions for the vast ma...
The significance of mutualisms between eukaryotic hosts and microbes extends from the organismal to the ecosystem level, and mutualistic symbioses underpin the health of Earth’s most threatened marine ecosystems. Despite rapid growth in research on host-associated microbes (microbiomes), very little is known about their interactions for the vast ma...
Based on modern microbiology, we propose a major revision in current space exploration philosophy and planetary protection policy, especially regarding microorganisms in space. Mainly, microbial introduction should not be considered accidental but inevitable. We hypothesize the near impossibility of exploring new planets without carrying and/or del...
Resumo O objetivo deste estudo foi avaliar as condições higiênico-sanitárias e o perfil da comunidade microbiana dos utensílios e das mesas de um serviço de alimentação localizado no município do Rio de Janeiro. A caracterização do processo de higienização dos utensílios (pratos, bandejas e talheres) e das mesas foi realizada por observação sistemá...
Although numerous studies have been carried out on the impacts of oil spills on coral
physiology, most have relied on laboratory assays. This scarcity is partly explained by
the difficulty of reproducing realistic conditions in a laboratory setting or of perform‐
ing experiments with toxic compounds in the field. Mesocosm systems provide the
opport...
Light microscopy micrograph of a thin section of M. hispida bundles, highlighting male and female gametes. F: female gametes. M: spermatic cysts.
Scheme showing the experimental strategy used in this study. Boxes in gray scale: collected samples are represented from embryonic stage (bundles, spawning time) to coral planula larvae (3 and 5 days a.f.). Blue rectangles: techniques used in this study; bacterial communities profile and composition were evaluated by PCR-DGGE and sequencing, respec...
Bacterial core community of different life stages of M. hispida at OTU level. G0d: bundles at the spawning event, L5d: coral planula larvae, 5 days a.f., and MH: adult colonies of M. hispida.
Richness indexes for bacterial communities associated with different life stages of Mussismilia hispida and surrounding seawater. (A) Phylogenetic distance, (B) Chao and (C) Observed OTUs. W0d: seawater at the spawning event, W5d: seawater 5 days after the spawning event, G0d: Bundles at the spawning event, L5d: coral planula larvae, 5 days a.f., a...
Relative abundance of the variable and core microbiome from different M. hispida life stages, according to 16S rRNA gene high throughput (Ion Torrent PGM) sequencing data. G0d: bundles at the spawning event, L5d: coral planula larvae, 5 days a.f., and MH: adult colonies of M. hispida.
Permutational statistical (PERMANOVA) analyses of DGGE and sequencing data to assess the water and Mussismilia hispida microbiome.
Figure S2. Inside view of control room, showing the Reef Angel cabinet, the pH sensors, and precision gate valves for flow control.
Figure S1. Panoramic view of the mesocosm system as observed from the secondary ecotoxicology system aquaria.
Table S1. Average abiotic parameters concentrations from each treatment of mesocosm raceway tanks (n = 4 replicates).
Table S2. Summary of values for pCO2 and Ωaragonite parameters during acidification system experiment.
Citations
... In line with even moderate projections of global warming, 70-90% of coral reefs may disappear in the coming decades 5,6 , jeopardizing the biological diversity they support and the more than 500 million people who rely on the services they provide 7 . To minimize further losses, and maximize their potential for recovery, the implementation of effective conservation strategies for these ecosystems is imperative [8][9][10] . Moreover, developing adaptation strategies and building increased reef resilience based on the natural adaptive potential of corals has become a requirement to ensure a future for coral reefs 5,9 . ...
... The dominant bands from each distinctive DGGE fingerprint profiles were excised, re-amplified, and directly sequenced on an Applied Biosciences sequencer (Applied Biosciences, Foster City, CA) at the Pennsylvania State University Genomics Core facility. Therefore, putative species were assigned based on the dominant or co-dominant ITS2 sequence [38]. Second, the nuclear large-subunit ribosomal DNA (LSU) was amplified and sequenced from a subset of samples using methods described by Zardoya et al. [39] to verify taxonomic identity. ...
... Lastly, while a marginalized fish community could suffice to keep coral nurseries free of fouling, the protection of the full fish community or, potentially, restocking of key species (Abelson et al. 2016) will likely provide additional benefits for reef restoration including nutrient recycling, increased functional redundancy and more effective grazing pressure around outplanted corals. Therefore, we consider the integration of restoration and protection the most effective way forward to rehabilitate and preserve functional coral reefs locally, while global stressors are being addressed simultaneously (Knowlton et al. 2021). ...
... Bacteria protecting their host is a known phenomenon in various natural systems [33,46,47]. Our study is the first to show this behavior in algal-bacterial interactions. ...
... where sequence reads were processed using a mothurbased pipeline (Schloss et al., 2009), taxonomically screened using blast with a SymPortal-curated Symbiodiniaceae database, and finally decomposed using minimum entropy decomposition (med) (Eren et al., 2015). SymPortal predicts ITS2 type profiles (Davies et al., 2022) as a proxy for putative Symbiodiniaceae taxa by identifying sets of sequences, referred to as defining intragenomic sequence variants (DIVs), found in common between multiple samples. Profiles recovered from P. verrucosa had a larger number of DIVs in common with their next most similar profile than in S. pistillata (3.50 vs. 2.01 DIVs in common for P. verrucosa and S. pistillata, respectively). ...
... While sexual reproduction has been relatively well studied in cnidarians, particularly coral spawning, causal factors that affect rates of asexual reproduction are not yet well characterized in any species [2], and most studies are descriptive-focusing on how the process of asexual reproduction occurs (e.g. budding, fissioning) [3][4][5]. ...
... These hazardous compounds may endanger aquatic life after being released into the environment, mostly through sewage discharge [1]. According to several research investigations, micropollutants and their metabolites are extensively distributed in urban aquatic habitats, which has raised global concerns [2][3][4][5][6]. Humans as well as livestock are often treated with pharmaceuticals and hormones; nevertheless, these drugs have the potential to contaminate groundwater. ...
... Nevertheless, SWA reefs have been highlighted as less susceptible to mortality from bleaching than the Caribbean and Indo-Pacific reefs due to characteristics, such as water turbidity, the depth range of coral species, the dominance of more resilient massive corals, and the generalism of symbiosis patterns, including the dominance of generalist host species and symbiont lineages [21] . In addition, many studies have revealed a high diversity of symbiodiniaceans associated with SWA corals, following Caribbean patterns [22][23][24][25] . However, the dynamics of these diverse photosymbiont assemblages during bleaching events are still poorly understood. ...
... capacities for restoration (de Jesus et al., 2015;Berg et al., 2021;Peixoto et al., 2021), biotechnological application (Cross et al., 2019;Modolon et al., 2020;Vilela et al., 2021), and promotion of organismal health and resilience (Sanders et al., 2019;Woodhams et al., 2020;Berg et al., 2021;Peixoto et al., 2021), predominantly from a anthropocentric point of view (Cavicchioli et al., 2019). ...
... Fortunately, next-generation sequencing methods, such as RADseq (Arrigoni et al. 2020, Wepfer et al. 2020 and targeted capture of conserved loci (Quattrini et al. 2018, Cowman et al. 2020, Quek et al. 2020, Ramirez-Portilla et al., 2022a, which examine a much larger number of independent markers, are proving more effective at resolving species-level relationships in previously problematic coral taxa. These powerful molecular tools, combined with increasing access to type material and original descriptions through digitization of museum collections (Beaman and Cellinese 2012), provide the opportunity to develop a robust taxonomy for reef corals (Bonito et al. 2021, Voolstra et al. 2021. ...