Rahul M. Jawale's research while affiliated with University of Massachusetts Medical School and other places

Publications (15)

Article
Full-text available
While changes in DNA methylation are known to occur early in breast carcinogenesis and the landscape of breast tumour DNA methylation is profoundly altered compared with normal tissue, there have been limited efforts to identify DNA methylation field cancerization effects in histologically normal breast tissue adjacent to tumour. Matched tumour, hi...
Article
DNA methylation plays a role in the etiology of primary breast cancers. We analyzed paired primary and second breast tumors to elucidate the role of methylation in recurrence. Methylation profiles from paired primary and second breast tumors of 23 women were assessed using the HumanMethylation450 BeadChip. Twelve women had estrogen receptor positiv...
Article
Full-text available
Background The DNA methyltransferase 1 inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-dC) is a potential treatment for breast cancer. However, not all breast tumors will respond similarly to treatment with 5-Aza-dC, and little is known regarding the response of hormone-resistant breast cancers to 5-Aza-dC. Methods We demonstrate that 5-Aza-dC-treatment h...
Article
Full-text available
Background: Most women with primary breast cancers that express estrogen receptor alpha (ER or ESR1) are treated with endocrine therapies including the anti-estrogen tamoxifen, but resistance to these anti-endocrine therapies often develops. This study characterizes the expression of hormone receptors, and the mRNA and DNA methylation levels of do...
Article
Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA Women who receive adjuvant tamoxifen for estrogen receptor (ER) positive breast cancer may develop drug resistant tumors through several pathways. DNA methylation of CpG islands in gene promoters is a mechanism of gene silencing, which has been implicated in acquired tamoxifen r...

Citations

... To further examine how the 3D chromatin architectures of TNBC tissues are reorganized compared to those of normal tissues, we collected primary tumor tissues from TNBC patients (Supplementary Table 9) and investigated the 3D chromatin organization and transcriptomic changes by comparing these samples with normal tissues. In previous studies on breast cancer, tumor-adjacent histologically normal tissues (Fig. 6a, adjacent normal) have frequently been used as controls [45][46][47] . However, several reports have shown that the accumulation of genetic abnormalities, known as the cancerization effect, is often observed in adjacent normal tissues 45,46,48 . ...
... In addition to cfDNA gene sequence and mutation, cfDNA can be further analyzed for epigenetic alterations, such as DNA methylation, histone modifications, and expression of long and micro non-coding RNAs [51,52]. Methylation changes in DNA contribute to gene expression regulation and play a significant role in the etiology of early breast cancer [53,54]. The DNA methylation pattern is retained in the cfDNA released from its tissue origins of tumor cells [55,56]. ...
... Trop-2 expression has also been described in estrogen receptor (ER)-positive breast cancers [1,[37][38][39], although it appeared to be lower than in TNBC. This was observed in breast cancer cell lines [40] and in breast tumor samples [14,38,39,[41][42][43] (Figure 1). Recent data also indicate a promising therapeutic activity of SG in patients with luminal (ER+) subtype of breast cancer [44]. ...
... It is a cytoplasmic activator of the muscle-speci c kinase (MuSK), involved in neuromuscular junction formation and maintenance [11]. CpG site hypermethylation was observed within the DOK7 promoter region in cancers with epithelial origins, such as breast [12] and lung [13] cancers. SPDEF (SAM Pointed Domain containing ETS transcription factor) is an ETS family transcription factor reported to playing a role in tumor progression and several cancers' metastasis. ...
... Collection and processing of human breast tumors was conducted with IRB approval from Baystate Medical Center and the University of Massachusetts as previously described [30]. Breast tumor samples purified using the BiOstic FFPE tissue DNA isolation kit (Mo Bio, Carlsbad, CA) were sent to the core facility at the University of Southern California for HM450 BeadChip (Illumina) analysis and detailed results are in preparation (Williams in prep). ...