Raffaele Peduzzi's research while affiliated with University of Geneva and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (57)
Here, we present sedimentological, trace metal, and molecular evidence
for tracking bottom water redox-state conditions during the past 12,500
years in nowadays sulfidic and meromictic Lake Cadagno (Switzerland). A
10.5 m long sediment core from the lake covering the Holocene period was
investigated for concentration variations of the trace metals...
Tapeworms (cestodes) of the genus Diphyllobothrium are widely distributed all around the world, some of which are agents of human diphyllobothriosis, one of the most important fish-borne zoonoses. Piscivorous birds and mammals (including humans) are definitive hosts and contract these parasites by eating raw or poorly cooked freshwater or marine fi...
Two isolates, designated CadH11(T) and Cad448(T), representing uncultured purple sulfur bacterial populations H and 448, respectively, in the chemocline of Lake Cadagno, a crenogenic meromictic lake in Switzerland, were obtained using enrichment and isolation conditions that resembled those used for cultured members of the genus Thiocystis. Phenoty...
The aim of this study was to compare the composition of bacterial and archaeal communities in contaminated sediments (Vidy Bay) with uncontaminated sediments (Ouchy area) of Lake Geneva using 16S rRNA clone libraries. Sediments of both sites were analysed for physicochemical characteristics including porewater composition, organic carbon, and heavy...
Human diphyllobothriosis is caused by at least 14 species of cestodes belonging to the genus Diphyllobothrium. Molecular analysis by sequencing of nuclear and mitochondrial targets identifies some species at inter- and intra-specific level, and helps to reconstruct their phylogenetic relationships. Nevertheless, the suitability of further molecular...
In recent years, human diphyllobothriosis has staged a comeback in Swiss, French and Italian sub-alpine regions. The main putative infective source of the causative agent (the tapeworm Diphyllobothrium latum) in these areas is perch (Perca fluviatilis). Therefore, the occurrence of D. latum in this fish species was investigated between 2005 and 200...
Diphyllobothriosis affects man and mammals as definitive hosts, as well as several fish species as intermediate or paratenic hosts. In Italy, in the last decade, Diphyllobothrium latum (Cestoda, Diphyllobothriidea) has shown a comeback around the shores of Lake Lario (Como), with several cases diagnosed from 2001. Anamnestic data constantly reporte...
Antibiotic resistant bacteria and antibiotics are discharged in various amounts in the environment as a result of the increasing and often indiscriminate use of antibiotics in medical, veterinary and agricultural practices. Surface waters are the main receptacle for these pollutants and are the major source of water, directly or indirectly used for...
Five Aeromonas strains, isolated from both clinical and environmental sources and characterized by a polyphasic approach, including phylogenetic analysis derived from gyrB, rpoD, and 16S rRNA gene sequencing, as well as DNA-DNA hybridization, extensive biochemical and antibiotic susceptibility tests, were recognized as members of an unknown, or und...
The tapeworm Diphyllobothrium nihonkaiense (Cestoda: Diphyllobothriidea), originally described from Japan, is reported from a man in North America for the first time. Species identification was based on sequences of ribosomal (partial 18S rRNA) and mitochondrial (partial Cytochrome c Oxidase subunit I) genes of proglottids expelled from a Czech tou...
We report for the first time in Switzerland a clinical case because of Diphyllobothrium dendriticum, identified by molecular methods. We discuss the potential for this imported species to infect local intermediate hosts and thus to achieve autochthonous cyclic transmission.
A 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of Aeromonas sp. in the water. An isolate of Aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. The cau...
We report the first cases of locally-acquired Diphyllobothrium nihonkaiense (Yamane, Kamo, Bylund and Wikgren, 1986) in Switzerland, confirmed by genetic analysis (18S rRNA, COI and ITS1-5.8S rRNA-ITS2 genes). Diphyllobothriasis in this country is attributed to the tapeworm D. latum (Linnaeus, 1758) but the increasing popularity of raw fish culinar...
Genetic relationships among bacterial strains belonging to the genus Aeromonas were inferred from 16S rRNA, gyrB and rpoB gene sequences. Twenty-eight type or collection strains of the recognized species or subspecies and 33 Aeromonas strains isolated from human and animal specimens as well as from environmental samples were included in the study....
Comparative sequence analysis of almost complete 16S rRNA genes of members of the Desulfobacteriaceae retrieved from two gene clone libraries of uncultured bacteria of the chemocline of Lake Cadagno, Switzerland, resulted in the molecular identification of nine sequences, with a tight cluster of five sequences that represented at least three differ...
Population analyses in water samples obtained from the chemocline of crenogenic, meromictic Lake Cadagno, Switzerland, in
October for the years 1994 to 2003 were studied using in situ hybridization with specific probes. During this 10-year period,
large shifts in abundance between purple and green sulfur bacteria and among different populations wer...
A previously described sequence-based epidemiological typing method for clinical and environmental isolates of Legionella pneumophila serogroup 1 was extended by the investigation of three additional gene targets and modification of one of the previous targets.
Excellent typeability, reproducibility, and epidemiological concordance were determined...
Lake Cadagno, a crenogenic meromictic lake located in the catchment area of a dolomite vein rich in gypsum in the Piora Valley in the southern Alps of Switzerland, is characterized by a compact chemocline with high concentrations of sulfate, steep gradients of oxygen, sulfide and light and a turbidity maximum that correlates to large numbers of bac...
Diphyllobothriasis, a parasitosis caused by the flatworm Diphyllobothrium latum, is contracted by consuming raw or undercooked freshwater fish. The aim of this study was to evaluate the situation of this parasitosis during the past 20 years in Europe through the analysis of databases and search engines (Medline, Cabi Helminthological abstracts,Yaho...
In the current study, a group of 17 "Aeromonas eucrenophila-like" isolates from clinical and environmental origins was subjected to a polyphasic taxonomic characterization including ribotyping, 16S rDNA sequencing, FAFLP fingerprinting, and biochemical testing. Genotypic, phylogenetic, and phenotypic results were consistent with the conclusion that...
We report a case of endocarditis due to Arthrobacter woluwensis and review the published reports of Arthrobacter species isolated from human clinical samples. A 39-year-old injection drug user presented with fever and a new heart murmur.
A. woluwensis was isolated from blood cultures, and a diagnosis of subacute infective endocarditis of the native...
Seven gene loci of Legionella pneumophila serogroup 1 were analyzed as potential epidemiological typing markers to aid in the investigation of legionella outbreaks.
The genes chosen included four likely to be selectively neutral (acn, groES, groEL, and recA) and three likely to be under selective pressure (flaA, mompS, and proA). Oligonucleotide pr...
Abstract In situ hybridization was used to study the spatio-temporal distribution of phototrophic sulfur bacteria in the permanent chemocline of meromictic Lake Cadagno, Switzerland. At all four sampling times during the year the numerically most important phototrophic sulfur bacteria in the chemocline were small-celled purple sulfur bacteria of tw...
To assess the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in Switzerland.
One-year national survey of all MRSA cases detected in a large sample of Swiss healthcare institutions (HCI). Analysis of epidemiological and molecular typing data (PFGE) of MRSA strains.
During 1997, 385 cases of MRSA were recorded in the 5 university...
The effects of different sewage treatments on the viral contamination in rivers which receive water from treatment plants without a final sand filtration step were investigated. They were all heavily contaminated with bacteriophages. and human enteric viruses (detected by single step reverse transcription amplification followed by a nested polymera...
Lake Cadagno is a 21 m deep alpine meromictic lake situated at an altitude of 1921 m in the Piora valley in the southern part of central Switzerland. The bedrock of the valley containing dolomite and gypsum determines the chemistry of the water. The lake basin was created by glacial erosion and originally dammed by a glacial moraine. The water body...
The secretory immunoglobulin A (sIgA) response at the intestinal mucosa is one of the primary defense mechanisms protecting against enteric infections and may therefore be used as an indicator of bacterial enteropathogenicity. In order to understand the role played by Aeromonas strains as gastrointestinal infectious agents, the sIgA response in fec...
Stool specimens from children (<4 years old) with diarrhea were collected over a 1-year period in Ticino (southern region of Switzerland). During the same period, environmental samples were collected from surface waters in the proximity of major water treatment plants. From treatment plants, samples were collected from the raw sewage and before the...
Ribotyping was used to study the epidemiology of Aeromonasassociated gastro-enteritis in young children. Ribotyping patterns of 29 Aeromonasstrains (16 Aeromonas caviae, 8 Aeromonas hydrophila, 3 Aeromonas eucrenophila, 1 Aeromonas veronii, and 1 Aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the GelC...
Comparative sequence analysis of a 16S rRNA gene clone library from the chemocline of the meromictic Lake Cadagno (Switzerland)
retrieved two clusters of sequences resembling sulfate-reducing bacteria within the family Desulfovibrionaceae. In situ hybridization showed that, similar to sulfate-reducing bacteria of the familyDesulfobacteriaceae, bact...
We found 73.1 to 96.9% similarity by aligning the cytolytic enterotoxin gene of Aeromonas hydrophila SSU (AHCYTOEN; GenBank accession no. M84709) against aerolysin genes of Aeromonas spp., suggesting the possibility of selecting common primers. Identities of 90 to 100% were found among the eight selected primers from those genes. Amplicons obtained...
Comparative sequence analysis of a 16S rRNA gene clone library from the chemocline of the meromictic Lake Cadagno (Switzerland) revealed the presence of a diverse number of phototrophic sulfur bacteria. Sequences resembled those of rRNA of type strains Chromatium okenii DSM169 and Amoebobacter purpureus DSM4197, as well as those of four bacteria fo...
Lake Cadagno is ideally suited for ecological studies and for investigations on metabolic responses of aquatic bacteria, especially of the phototrophs, to environmental signals such as light and environmental redox conditions. The dynamics of the water chemistry and the special conditions which follow from it, are consequences of geological feature...
To study the population's composition of the bacterioplankton observed in the monimo- limnion of the Lake Cadagno, we isolated the genes encoding the small subunit ribosomal RNA from a water sample taken at 17 meters depth, using PCR universal primers for the do- main Bacteria. After cloning in a plasmid vector, 276 clones containing the 1.4 kb ful...
Bacterioplankton was analyzed in monthly intervals from June to September in water samples from the meromictic Lake Cadagno (Switzerland) that was characterized by a per- manent chemocline separating the aerobic epilimnion from the anaerobic, sulfidogenic hy- polimnion. During the whole period, the physico-chemical parameters showed the character-...
While studying the microbial flora of Lake Cadagno, a meromictic alpine lake, a peculiar morphological group of bacteria, named "morphotype R", was observed. This group of bacte- ria was present only in the anoxic monimolimnion. Its distribution in the water column could be correlated with the hydrogen sulfide content and the redox potential. Attem...
To study nutrient fluxes within aquatic ecosystems, the synthesis of biomass and of various storage polymers has been analysed in samples from a meromictic alpine lake. Methods are described for the quantitative determination of whole cell biomass, glycogen, polyhydroxyalkanoates (PHA) and sulfur. Methods were adapted to conditions present in natur...
This research was carried out with the aim to explore the heterotrophic microbial population of two sediments in different oxic conditions of the Lake of Lugano (Lago di Lugano). The values of the viable bacterial counts found in our sediment samples were typical for an eutrophic lake.
The increase in the proportion of anaerobic to aerobic bacteria...
Allelic variations in the chromosomal genome of 120 isolates of motile Aeromonas (A. hydrophila, A. sobria and A. caviae) and eight reference strains with one Plesiomonas shigelloides were assessed by analysis of electrophoretically demonstrable polymorphism in 16 genes encoding metabolic enzymes. The strains were collected from humans (n = 59) and...
Citations
... Subaquatic springs within the Triassic rocks that dip below the lake maintain a flow of solute-rich groundwaters to the lake. As a result, a strong density gradient exists in the water column leading to permanent stratification with an oxic mixolimnion, an anoxic monimolimnion, and a chemocline at a depth of 10 to 14 m separating the two water masses (Del Don et al., 2001). Fig-ure 1b shows water-column profiles of dissolved O 2 and H 2 S, as well as the electrical conductivity and water temperature, demonstrating the contrasting chemical and physical properties of Lake Cadagno above and below the chemocline (Dahl et al., 2010). ...
... All rights reserved. and human safety (Corvaglia et al., 2008;Xu et al., 2011;Manzetti and Ghisi, 2014;Lee et al., 2014;Iliev et al., 2015;Jahne et al., 2015). ...
... Dibothriocephalus dendriticus) plerocercoids in fish has been identified at 83.2% and 8.8% [41] and D. latum (syn. D. latus) the mean infection intensity has been identified as low as 1.25 parasites/fish [42]. ...
... Dispersal of adult Ae. albopictus by ground vehicles (e.g. trucks and private vehicles) from Italy is believed to have resulted in the dispersal of this species into Switzerland, Slovenia, San Marino, the Czech Republic, Croatia and Germany [16][17][18][19][20][21]. In Spain, dispersal of adult Ae. albopictus inside private vehicles across the country was directly observed in multiple instances [5], likely contributing to the rapid spread of Ae. albopictus throughout Spain since its first detection in 2004. ...
... In this study, we investigate the sediments of Lake Cadagno, a meromictic lake in the Swiss Alps with a wellstudied sedimentary record (Wirth et al., 2013;Berg et al., 2022) and known for its dense populations of diverse phototrophic sulfur bacteria (Tonolla et al., , 2005Peduzzi et al., 2012Peduzzi et al., , 2011. Therefore, it is an ideal site to test the feasibility of using HSI to measure GSB-related bacteriochlorophylls in sediments that contain a mixture of pigments from both oxic and anoxic phototrophs. ...
... Parallel to this evolution, Lake Cadagno sediments have changed from organic-poor, oxic glacial clays to organic-rich, anoxic sediments. The transition from fully mixed, oxygenated waters to stratified, permanently anoxic bottom waters was driven by the inflow of saline sulfidic subaquatic springs since the end of the last glacial stage (Del Don et al., 2001;Wirth et al., 2013). The presence of anoxic sulfidic conditions, together with anoxygenic photosynthesis, has made Lake Cadagno a famous analog for oceanic conditions on the early Earth (Dahl et al., 2010;Wirth et al., 2013;Xiong et al., 2019). ...
... Desulfomonile tidjei in the monimolimnion of Lake Cadagno is easily recognizable as it emits a green fluorescence after acridine orange staining (Bensadoun et al. 1998) and is detectable with the two specific probes DsmA445 and DsmB455 (Tonolla et al. 2005a). Its vertical distribution was unusual, compared with other SRB, as it did not produce a maximum in abundance in the chemocline, but its distribution pattern followed that of hydrogen sulphide profiles. ...
... Pure cultures of ''morphotype R'', however, are not available yet and attempts to characterize ''morphotype R'' by molecular methods such as, e.g., by in situ hybridization with probes commonly used to analyze populations of sulfate-reducing bacteria on a lower taxonomic level [23,24] were not successful [17]. The aim of this study was therefore to take advantage of the availability of two gene clone libraries of almost complete 16S rRNA genes retrieved by PCR from DNA samples obtained from uncultured bacteria of the chemocline [25] in order to phylogenetically characterize ''morphotype R'' unaffected by the limitations of culturability. After comparative sequence analysis of clones selected after hybridization with probes SRB385Db and DSS658 targeting members of the Desulfobacteriaceae , specific oligonucleotide probes were designed and used to evaluate the significance of the sequences in environmental samples. ...
... based on previous descriptions in the literature (e.g. ref. 35 ). Other bacteria were also present, however only in low numbers (<10%). ...
... Although surface sulfide minerals can have diverse origins, sedimentary processes predominate over magmatic, hydrothermal, and volcanic ones . In modern environments, sulfide formation is mainly driven by the metabolism of sulfatereducing microorganisms (SRM), which are prevalent in anoxic environments, e.g., marine sediments or water column of permanently stratified euxinic waters such as the Black Sea (Vetriani et al., 2003) or Lake Cadagno (Tonolla et al., 2004) or even in ferruginous environments such as Lake Pavin (Lehours et al., 2005;Berg et al., 2016;Berg et al., 2019). Understanding the mechanisms of a possible biogenic pyrite formation pathway is crucial as pyrites are used as paleoenvironmental proxies and might also be good candidates for the search of biosignatures of early life (Shen and Buick, 2004). ...