Rachel B. Currier's scientific contributions
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Publications (8)
Raw data following gene expression analysis by quantitative PCR. Gene expression analysis conducted using the BioRad CFX manager software. Relative gene expression was calculated from the cycle time (Ct value) using the ΔΔCt method. Expression profiles for all individual specimens in the study are shown illustrating fold changes in the expression o...
Optimisation of venom quantitative PCR. Representative standard curves for snake venom metalloproteinase (SVMP) and C-type lectin (CTL) (1Ai and Aii) show high efficiency amplification of 94.0 and 96.6% respectively. Representative melt curves for SVMP and CTL amplicons showing a single melt peak indicating a single specific amplicon (1Bi and Bii)....
Raw individual relative gene expression data generated by quantitative PCR. Raw qPCR data generated from relative expression analysis to show fold changes in expression of venom genes of interest, including snake venom metalloproteinase (SVMP), serine protease (SP), C-type lectin (CTL), Kunitz inhibitors (KTI), protein disulphide isomerase (PDI) an...
Minimum information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines. Guidelines published by Bustin et al 2009 were referred to in order to ensure accuracy and reliability of quantitative PCR data.
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