R.M.W. Mans's research while affiliated with University of Cologne and other places

Publications (12)

Article
Full-text available
During the infection cycle of Autographa californica multicapsid nuclear polyhedrosis virus, the TATA-binding protein (TBP) of the insect host cell likely participates in early viral transcription, which is mediated by the host RNA polymerase II. However, the role of TBP in late and very late viral transcription, which is accomplished by an alpha-a...
Article
Virulence and speed of action, as related to dose, are important effectiveness-determining properties of insect-pathogenic biocontrol agents. We used the droplet-feeding bioassay to compare dose responses between two wild-type baculoviruses, Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Spodoptera exigua MNPV (SeMNPV), and th...
Article
We have examined whether mitochondrial DNA could act as target of the RNA polymerase encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus, because the baculovirus late promoters and the control region of host mitochondrial DNA show a high degree of sequence similarity. In vitro transcription using mitochondrial D...
Article
Full-text available
In vitro transcription was used to analyze the promoter specificity of the alpha-amanitin-resistant RNA polymerase that is induced late during infection of Autographa californica multicapsid nuclear polyhedrosis virus. By modifying the preparation of crude nuclear extracts, we have established an assay that permits differentiation between weak late...
Article
Full-text available
The nucleotide sequence of a 1200 bp DNA fragment of Spodoptera exigua nucleopolyhedrovirus (SeMNPV) was determined. This sequence contained a cluster of two open reding frames (ORFs), one coding for a viral ubiquitin (v-ubi) and another with homology to orf2 of Autographa californica (Ac) MNPV and Orgyia pseudotsugata (Op) MNPV. The vubi ORF is 24...
Article
Full-text available
Deletion of the ecdysteroid UDP-glucosyltransferase gene (egt) from the Autographa californica nuclear polyhedrosis virus (AcNPV) genome increases the speed of killing of this virus (D. R. O'Reilly and L. K. Miller, Bio/Technology 9:1086-1089, 1991). Second-instar Spodoptera exigua larvae are killed more rapidly by the egt deletion mutant of AcNPV...
Article
Recombinant Autographa californica nuclear polyhedrosis viruses (AcNPV) were constructed to reduce their persistence in the field. A two-step cotransfection procedure was used to generate specific deletions in the genome of AcNPV. Used as an insecticide, such viruses are likely to cause less danger and damage to the environment when heterologous ge...

Citations

... The transcription of viral late and very late genes started in the conserved late and very late gene promoter motif sequence (A/T/G) TAAG. The TAAG sequence is an essential element for both polh and p10 promoters, which is also a common binding motif for viral polymerase [24]. The strength of hyperexpression of very late genes is dependent on an A/T rich sequence present in the downstream sequences of very late promoters [26]. ...
... Many members of baculoviruses encode the gene egt, which can interact with 20E in the host. Expression of egt suppresses the level of 20E and extends the host survival so as to increase the production of progeny virus (Flipsen et al., 1995;O'Reilly, 1995). The increase in bantam level after AcMNPV infection seems to have the same effect as egt, which could be another example of virus manipulating host physiology in favor of its own replication and dissemination. ...
... The lepidopteran conserved gene, polyhedrin, is known to be the major structural component of NPV OBs and thus is likely conserved to ensure viable OBs are formed (Rohrmann, 2013). Ubiquitin and sod are auxiliary genes involved in cell cycle response/stress response and virus entry/superoxide inactivation respectively (Li et al., 2003;Rohrmann, 2013;Van Strien et al., 1996). Based on the level of conservation it would appear these functions are significantly important in the virus infection cycle. ...
... The egt gene deletion in baculovirus increases the viral pesticide effectiveness significantly. Reduced feeding and earlier mortality was showed by egt deleted mutant of AcMNPV infected larvae than the wildtype AcMNPV infected larvae (Bianchi et al. 2000). The same approach also decreased the survival time of larvae infected with Lymantria dispar MNPV and H. armigera NPV. ...
... According to a recent study [33], in insects, TATA-binding protein (TBP) is associated with subunits of the viral RNA-dependent RNA polymerase complex (RdRp), which may indicate that TBP interacts with individual components of the viral RdRp complex for enhancing viral RNA replication. In sigma virus, the L gene encodes RdRp, whose main function is to regulate viral replication and translation. ...