January 1968
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19 Reads
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13 Citations
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January 1968
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19 Reads
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13 Citations
January 1953
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31 Reads
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782 Citations
Journal of Biological Chemistry
December 1951
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1,789 Reads
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161,445 Citations
Journal of Biological Chemistry
Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins (l), a number of modified analytical pro- cedures ut.ilizing this reagent have been reported for the determination of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and in insulin (10). Although the reagent would seem to be recommended by its great sen- sitivity and the simplicity of procedure possible with its use, it has not found great favor for general biochemical purposes. In the belief that this reagent, nevertheless, has considerable merit for certain application, but that its peculiarities and limitations need to be understood for its fullest exploitation, it has been studied with regard t.o effects of variations in pH, time of reaction, and concentration of react- ants, permissible levels of reagents commonly used in handling proteins, and interfering subst.ances. Procedures are described for measuring pro- tein in solution or after precipitation wit,h acids or other agents, and for the determination of as little as 0.2 y of protein.
November 1951
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18,351 Reads
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269,151 Citations
Journal of Biological Chemistry
January 1951
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25 Reads
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518 Citations
Journal of Biological Chemistry
January 1951
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79 Reads
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25,176 Citations
Journal of Biological Chemistry
January 1951
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33 Reads
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93 Citations
Journal of Biological Chemistry
January 1951
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27 Reads
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1,019 Citations
Journal of Biological Chemistry
January 1951
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11 Reads
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31 Citations
January 1951
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73 Reads
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1,139 Citations
Journal of Biological Chemistry
... One unit of LOX activity was defined as the amount of enzyme catalyzing the formation of 1µmol of the hydroperoxide per min under standard assay conditions. Protein concentration was determined by the method of Lowry et al., (1951). Bovine serum albumin (BSA) was used as standard. ...
January 1951
Journal of Biological Chemistry
... Protein content was measured using bovine serum albumin as the standard [20]: Solution A was prepared by dissolving 0.1 M NaOH and 2% Na2CO3, while Solution B consisted of 0.5% CuSO4·5H2O dissolved in a 1% sodium-potassium tartrate solution. Solution C, a mixture of Solution A and Solution B in a 50:1 ratio, was freshly prepared just before use. ...
January 1951
... Mitochondrial protein concentrations of iBAT were determined by Folin phenol method using bovine serum albumin as standard (Lowry et al., 1951). and goat anti-mouse IgG (1:5,000; ZSGB-BIO Co., Beijing, China). ...
January 1951
... The IAA (Indole acetic acid) production by isolates were determined via using colorimetric method in presence as well as in absence of tryptophan by using method of Brick et al. 29 Protein produced by bacterial isolates were estimated quantitively by using method of Lowry et al., 30 Values were determined with standard curve made by using concentrations of IAA ranging from 10-100 µg/ml separately for both IAA and Protein. Finally, the IAA production by isolates were estimated in microgram per gram (µg/g) of protein produced by bacterial isolates. ...
January 1951
Journal of Biological Chemistry
... The procedure described by Lowry et al. (1951) was used to extract the total protein content. Whereas, the procedure described by Moore and Stein (1948) was employed to obtain the total free amino acid content. ...
January 1951
Journal of Biological Chemistry
... Total soluble solids (TSS) content in leaves was estimated using a hand-held refractometer. Protein content in leaves was estimated using bovine serum albumin as a standard [15]. ...
November 1951
Journal of Biological Chemistry
... Total proteins and total carbohydrates for both T. aes0vum leaves and C. vulgaris microalga were es>mated as reported by Lowry et al. (1951) and Dubois et al. (1959), respec>vely. The lipid content of C. vulgaris has been determined by Bligh and Dyer method, (1959). ...
January 1951
Journal of Biological Chemistry
... Method of Lowry et al., 20 was used to determine protein content in each sample. The assay for LPO was carried out according to Islam et al., 21 method, whereas the assay for GSH performed according to Jollow et al., 22 method. ...
January 1951
Journal of Biological Chemistry
... Glutamic oxaloacetic transaminase (GOT) and glutamine pyruvic transaminase (GPT) were measured by the method of [21]. Proteins content was assayed using the Lowry et al. method [22] using bovine serum albumin (BSA) as standard protein, glycogen content and total lipids were measured by [23,24], respectively. ...
Current Science
... The results represented U/mg protein. The total protein contents of samples were evaluated based on Lowry's method [45]. ...
January 1953
Journal of Biological Chemistry