R.J. Randall's scientific contributions
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Publications (16)
Since 1922 when Wu proposed the use of the Folin phenol reagent for
the measurement of proteins (l), a number of modified analytical pro-
cedures ut.ilizing this reagent have been reported for the determination
of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and
in insulin (10).
Although the reagent would seem to be recommended...
Citations
... One unit of LOX activity was defined as the amount of enzyme catalyzing the formation of 1µmol of the hydroperoxide per min under standard assay conditions. Protein concentration was determined by the method of Lowry et al., (1951). Bovine serum albumin (BSA) was used as standard. ...
... carbohydrate, lipid, and astaxanthin chemical analyses163 Protein analysis following the method originally fromLowry et al. (1951). About 0.1 g of 164 powered macroalgae is extracted with trisodium phosphate (Na3PO4) (buffer pH = 0.7) and165 centrifuged. ...
... Mitochondrial protein concentrations of iBAT were determined by Folin phenol method using bovine serum albumin as standard (Lowry et al., 1951). and goat anti-mouse IgG (1:5,000; ZSGB-BIO Co., Beijing, China). ...
... Using an acid-alkali treatment method, the crude fibre content was analysed by weighing the sample's initial and final weights before and after ignition at 600 0 C. Using Nelson-Somogyi's arsenomolybdate reagent technique, reduced sugar is estimated. Protein was estimated using Lowry's (1951) technique. Oil was extracted using the Soxhlet method with Petroleum ether as the solvent, following the instructions provided by Blighand Dyer (1959). ...
... Total protein levels were assayed by the Lowry's (1951) method spectrophotometrically by measuring the absorbance at 700 nm. Bovine serum albumin (Merck 112018, Germany) was used as a standard. ...
... Measurements of SCOD, TCOD, TSS, VSS and pH were carried out according to Standard Methods (APHA, 2012). Quantification of proteins and humic-like substances was conducted using the modified Lowry method (Lowry et al., 1951), with bovine serum albumin (BSA) and humic acid as standards, respectively. The Dubois method was applied to determine carbohydrate content using D-glucose as a standard (DuBois et al., 1956). ...
... Whole hearts were homogenized and mitochondria were obtained by differential centrifugation, as previously described [26]. Mitochondrial total protein was quantified by the Lowry method [27]. ...
... Method of Lowry et al., 20 was used to determine protein content in each sample. The assay for LPO was carried out according to Islam et al., 21 method, whereas the assay for GSH performed according to Jollow et al., 22 method. ...
... Glutamic oxaloacetic transaminase (GOT) and glutamine pyruvic transaminase (GPT) were measured by the method of [21]. Proteins content was assayed using the Lowry et al. method [22] using bovine serum albumin (BSA) as standard protein, glycogen content and total lipids were measured by [23,24], respectively. ...
... The results represented U/mg protein. The total protein contents of samples were evaluated based on Lowry's method [45]. ...