R W Rosenstein's research while affiliated with Howard Hughes Medical Institute and other places

Publications (33)

Article
Previous studies have shown that T cell-dependent activation of mast cells to release serotonin is required for the elicitation of delayed-type hypersensitivity in mice. We have recently described an antigen-binding T cell factor that is a suitable candidate for participation in the mechanism by which T cells activate mast cells in delayed-type hyp...
Article
T cell-derived TNP-specific factors associated with immunoregulatory activity were obtained by culture of T cells obtained from mice sensitized by skin-painting with picrylchloride. Culture medium was absorbed to TNP-Sepharose and TNP binding proteins were prepared by elution with TNP. The hapten affinity-purified proteins were characterized by siz...
Article
Heterologous antisera to murine or rat T-cell antigen-binding molecules (T-ABM) were raised in rabbits or sheep. The T-ABM used for immunization were purified by affinity for antigen and did not bear known immunoglobulin isotypes. T-ABM and anti-T-ABM were raised in three separate laboratories. Antisera to T-ABM were exchanged and tested for bindin...
Article
Rabbits were immunized with TNP-specific Lyt-1+, 2- T cell-derived, antigen-binding proteins (PCI-F) released by T cells sensitized by skin painting with picrylchloride. The resulting antiserum (anti-PCI-F) bound to PCI-F and TNP-specific factors that suppressed delayed hypersensitivity (TSF) known to be comprised of PCI-F and Lyt-2+ -derived polyp...
Article
T cell-dependent activation of resident tissue mast cells is required for the elicitation of delayed-type hypersensitivity skin reactions in mice. A T cell-derived antigen-binding factor that transfers the ability to elicit an immediate hypersensitivity-like skin reaction is described and compared with a hybridoma IgE antibody. Both the T cell fact...
Article
T cells that have been immunized to express optimal levels of contact hypersensitivity upon adoptive transfer to normal animals can be inhibited from doing so by incubating them with an antigen-specific T suppressor factor. This factor is composed of at least two subunits which come from cells expressing different Ly phenotypes; an antigen-specific...
Article
The fact that T cell-dependent activation of mast cells occurs in delayed-type hypersensitivity led us to investigate whether a T cell product could mimic some of the functions of IgE. We report that 24- or 48-hr cultures of T cells from mice immunized optimally for delayed-type hypersensitivity resulted in release of an antigen-binding factor that...
Article
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Article
Full-text available
A single-cell secretion assay was used to detect cells that secrete products which react with an antiserum that binds T cell antigen-binding polypeptides. The antiserum (R11), which was produced by immunization of rabbits with a murine trinitrophenyl-specific suppressor factor, reacts with T cells and their products and with a suppressor T-cell clo...
Article
After immunization of mice with 2,4-dinitrophenyl-ovalbumin (DNP-OVA), it was shown previously that strains having Igh-Va genes and able to express light chains of the Vk1 group produce high levels of anti-DNP antibody bearing an idiotype (Id-460) associated with the combining site of the BALB/c DNP-binding myeloma protein MOPC 460. Expression of I...
Article
Heterologous antisera specific for murine T-cell antigen-recognition molecules were prepared by immunization of rabbits with dinitrophenyl-specific murine T-cell suppressor factors that had been purified by hapten-affinity chromatography. The antisera (i) bind to antigen-specific T-cell products that differ in their antigen-recognizing specificity;...
Article
Antigen-specific factors associated with immunosuppressive activity, released by cultured T cells from mice tolerant to the haptens trinitrophenyl, dinitrophenyl and oxazolone, were purified by hapten affinity chromatography. Their binding specificity for antigens paralleled their immunoregulatory activity. Like some immunoglobulin molecules, these...
Article
Radiolabelled cell membrane proteins obtained by detergent lysis of murine T cells labelled by lactoperoxidase-catalysed radioiodination were specifically immunoprecipitated with an antiserum to immunoglobulin Fab determinants. Absorption of the antiserum by myeloma proteins or normal immunoglobulins coupled to Sepharose and the use of antibody pro...
Article
The genetic contro of the expression of an idiotype (Id-460) associated with the 2,4-dinitrophenyl (DNP)-binding BALB/c myeloma protein MOPC 460 was studied using congenic strains of mice. It was shown that the expression of high levels of Id-460 during secondary in vivo anti-DNP-ovalbumin responses was determined by genes governing immunoglobulin...
Article
Sera directed against the public and private idiotypic determinants of protein 460 and protein 315 were used to detect the presence of these idiotypic markers in serum immunoglobulins of various inbred mouse strains. Private protein 315 determinants were not detected in any of the mouse strains tested. Presence of protein 460 private idiotype was c...
Article
The preparation and characterization of rabbit antibodies directed against structures associated with thehapten binding site of proteins 315 and 460 is described. That these antibodies are site-directed was shown by: (a) specific elution of anti-idiotypes by hapten; (b) the inability of these rabbit antibodies to bind to affinity labeled proteins 3...
Article
Both myeloma protein 460 and myeloma protein 315 each exhibit two sets of idiotypic determinants. A ‘private’ determinant set which distinguishes protein 460 and protein 315 from each other, and a common or ‘public’ set of idiotypic determinants which are shared by proteins 315 and 460 and which distinguish these two proteins from many other mouse...
Article
Hapten-specific delayed time course skin reactions containing predominant accumulations of basophils and eosinophils were elicited in newborn guinea pigs after i.v. transfer of small amounts of oxazolone immune serum. The immune serum was fractionated by column chromatography procedures, and the fractions were examined for their ability in transfer...
Chapter
An immune serum usually consists of a very heterogeneous population of immunoglobulins (Ig’s) the appearance of which in the serum has been induced by antigen. The antigen-ligating function of the Ig molecule (see Figure 1) is confined to the combining regions, which are two symmetrical areas at the solvent-exposed ends of the Fab arms of the Y-sha...
Article
Lactoperoxidase-catalyzed radioiodination was used to incorporate 125I or 131I into membrane proteins of murine thymocytes, spleen cells or spleen cells from mice depleted of T cells by adult thymectomy, X-irradiation and reconstitution with T cell-depleted bone-marrow cells (ATXBM mice). Radiolabelled cells were lysed with 0.05% Triton X-100 and t...
Article
Proteins 315 and 460 are IgA2 mouse myeloma immunoglobulins which bind the haptens 2,4-Dnp and Menadione, and their derivatives competitively in the combining region. We have studied the binding of these proteins to a series of Dnp and Menadione based solid phase immunoabsorbents with different length spacer groups to which the Dnp and Menadione gr...
Article
Protein 460 is a mouse γA2 myeloma immunoglobulin which binds competitively two haptens, ϵ-2,4 dinitrophenyl lysine and Menadione thioglycollate at the immunoglobulin combining region. In close relation to this combining region, protein 460 contains a heavy chain cysteine residue which has been substituted with 2 fluorescent donor probes. We have m...
Article
Proteins 315 and 460 are IgA2 mouse myeloma immunoglobulins which bind the haptens 2,4-Dnp and Menadione, and their derivatives competitively in the combining region. We have studied the binding of these proteins to a series of Dnp and Menadione based solid phase immunoabsorbents with different length spacer groups to which the Dnp and Menadione gr...
Article
Native multimeric protein 460, a γ A mouse myeloma protein, binds 8-anilinonaphthalene-1-sulfonate (ANS) with a K0 = 3 × 105 l./mol. Neither the 7S monomers nor the isolated J chain derived from this protein bind ANS. When 7S monomers and J chains are recombined, ANS binding is partially restored. The evidence is consistent with ANS binding to the...
Article
Full-text available
Protein 460 is a mouse myeloma gamma A(2) protein that competitively binds two small haptens, 2,4-epsilon-dinitrophenyl-L-lysine (Dnp-Lys) and 2-methyl-1:4-naphthaquinone thioglycollate (MenTG), to the antibody-combining region. The intact protein has a relatively inaccessible sulfhydryl group on each heavy chain. When it is substituted with a bulk...
Article
Photolytically derived carbenes were first used by Westheimer's group (1) as labeling agents for proteins. The carbenes and the related nitrenes (2) are useful agents for labeling receptor sites on proteins where a primary, non-covalent complex can be formed between protein and label. Such complexes can subsequently be triggered by near-UV light to...
Article
The role of bivalence of antibody in its capacity to neutralize virus was studied with rabbit antibodies to the bacteriophage, phiX174. Univalent Fab or Fab' fragments of IgG isolated from antiviral antisera obtained early in the immunization schedule had virtually no activity compared to that of the intact IgG. When the antibodies were isolated fr...
Article
The role of bivalence of antibody in its capacity to neutralize virus was studied with rabbit antibodies to the bacteriophage, ϕX174. Univalent Fab or Fab' fragments of IgG isolated from antiviral antisera obtained early in the immunization schedule had virtually no activity compared to that of the intact IgG. When the antibodies were isolated from...

Citations

... The specific activity of the abJaF hybrid, based on its capacity to bind cytochrome bs, was found to vary from 19.2 to 25.8 U per mg of IgG protein. The theoretical specific activity value, however, is approximately 80. Several possible explanations of this difference are: (a) the amount of anti-cytochrome b5 activity is underestimated because of a lower affinity of monovalent antibodies (38), (b) a large portion of the recovered antibody is denatured, or (c) the immunoadsorbent columns are not producing pure antibodies, and therefore a significant proportion of the hybrid preparation may not be of the abs/aF species. The first explanation was tested by diluting an anti-cytochrome b5 preparation four times with normal IgG to obtain asp act of 6.1. ...
... Mφ are involved as antigen-presenting cells and effector cells in delayed-type hypersensitivity reactions, including CS, as well as being able to induce a humoral immune response to corpuscular antigen. Previous studies reported the ability of Mφ to bind suppressive exosomes 5 and suggested their important role in the currently investigated suppression mechanism. [6][7][8][9][10][11][12] Current studies aimed to investigate the role of Mφ in Ts cell-derived exosome-mediated suppression of the immune response as well as to determine the ability of assayed exosomes to modulate the antigen-presenting function of Mφ in the induction of humoral and cellular immunity. ...
... Germline antibody diversity is generated during the recombination of Variable (V), Diversity (D), and Joining (J) gene segments (1), which may yield over 10 13 unique antibodies that are limited by the approximately 10 11 B cells in circulation at a given time (2). Despite the enormity of this repertoire, it is ultimately finite, so at least some antibodies must be able to recognize many different antigens to protect against the almost limitless potential for antigenic diversity in the environment (3). ...
... have been previously characterized in our laboratories (16)(17)(18)(19). The present experiments demonstrate several interesting points about Id-460. ...
... We examined the responses to both a haptenspecific monoclonal IgM bearing the M460 Id (Id § antibody or Ab 1), and to an anti-M460 Id mAb (representing anti-Id or Ab2) [16,17]. The M460 Id has been previously characterized in terms of its expression [9, 32,33], germline VH gene family [34], and associated reactivity [35]. ...
... It was originally reported that the adoptive transfer of lymphocytes, most likely T cells, but not serum from sensitized guinea pigs causes CBH in naïve animals [38], in a manner analogous to DTH, but distinct from the antibody-mediated, immediate hypersensitivity reaction. However, later studies showed that CBH can be induced by the passive transfer of IgG1 or IgE from sensitized guinea pigs to naïve ones [39,40]. Thus, the mechanism by which the CBH response, including basophil recruitment, is triggered remains uncertain, as does the role of basophils in CBH. ...
... This in itself should occasion no surprise since the culture medium containing bovine serum is likely to have in it cross-reacting substances that like monovalent haptens are rendered immunogenic by LPS. In view of the possibility that cross-reactions could be quite extensive (32,34), the assumption is not unreasonable that the in vitro culture contain a large number of determinants recognizable by B lymphocyte receptors. ...
... The E18 and E19 antibodies were prepared by immunizing mice with empty, immature EV71 particles containing VP0 (18). Both E18 and E19 could neutralize the virus as intact antibodies or as Fab fragments (Fig. 1). ...
... Very importantly, suppression was restored by coating of these non-suppressive exosomes from tolerized JH −/− mice with monoclonal Ab light chains [2]. Of related translational clinical significance, in our prior work on immediate hypersensitivity, on models of CHS [198] and asthma [199], induced by cutaneous sensitization with hapten or protein [200], the rapid activation of the small subpopulation of Ag-specific special B1a cells induced early after immunization was observed in all of these instances. Their activation resulted in production of Ag-specific IgM Ab and the Ag-specific Ab light chains that coat the Ts cellderived exosomes and that have the additional ability to sensitize cells; so far principally mast cells by surface coating for mediator release when Ag is added, in an analogous manner to IgE Ab [201]. ...