R Mathur's research while affiliated with Christian Medical College Vellore and other places

Publications (5)

Article
Neutral alpha-D-mannosidase from monkey brain was purified by Co2+-chelate affinity chromatography and immunoadsorbent affinity chromatography. The purified enzyme, with subunit Mr 45,000, was essentially homogeneous with only traces of two contaminant proteins as revealed by SDS/polyacrylamide-gel electrophoresis and AgNO3 staining. The purified e...
Article
Lysosomal alpha-D-mannosidase of monkey brain existed in two forms. One form of mannosidase was bound to the Ricinus communis agglutinin120 (RCA1)-Sepharose and could be specifically eluted with lactose. The other form did not bind to the RCA1-Sepharose. Both forms of mannosidase could bind to a similar extent to the immobilized brain lysosomal rec...
Article
A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single ste...
Article
Full-text available
Concanavalin A bound to Sepharose has been used for the purification of brain β-galactosidase, α-L-fucosidase, α-D-mannosidase, arylsulphatase and β-glucuronidase.0 Several factorsviz pH, temperature and concentration of α-methyl glucoside influenced the binding and elution of these enzymes. A lysosomal acid α-mannosidase and a cytosolic neutral ma...

Citations

... The ''cytosolic'' neutral a-mannosidase, referred to as Man2C1, occurs in various animal sources [152][153][154][155][156][157][158][159][160][161][162][163][164][165]. It has been shown that the knockout of Man2C1 in mice results in a dramatic accumulation of Gn1 type of FNGs with Man 8-9 (Man 8-9 GlcNAc 1 ) in the cytosol of all tissues tested [166], strongly indicating that the action of Man2C1 is responsible for generating M5B 0 in the cytosol (Fig. 3). ...
... The ''cytosolic'' neutral a-mannosidase, referred to as Man2C1, occurs in various animal sources [152][153][154][155][156][157][158][159][160][161][162][163][164][165]. It has been shown that the knockout of Man2C1 in mice results in a dramatic accumulation of Gn1 type of FNGs with Man 8-9 (Man 8-9 GlcNAc 1 ) in the cytosol of all tissues tested [166], strongly indicating that the action of Man2C1 is responsible for generating M5B 0 in the cytosol (Fig. 3). ...