R Mathur's research while affiliated with Christian Medical College Vellore and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (5)
Neutral alpha-D-mannosidase from monkey brain was purified by Co2+-chelate affinity chromatography and immunoadsorbent affinity chromatography. The purified enzyme, with subunit Mr 45,000, was essentially homogeneous with only traces of two contaminant proteins as revealed by SDS/polyacrylamide-gel electrophoresis and AgNO3 staining. The purified e...
Lysosomal alpha-D-mannosidase of monkey brain existed in two forms. One form of mannosidase was bound to the Ricinus communis agglutinin120 (RCA1)-Sepharose and could be specifically eluted with lactose. The other form did not bind to the RCA1-Sepharose. Both forms of mannosidase could bind to a similar extent to the immobilized brain lysosomal rec...
A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single ste...
Concanavalin A bound to Sepharose has been used for the purification of brain β-galactosidase, α-L-fucosidase, α-D-mannosidase,
arylsulphatase and β-glucuronidase.0 Several factorsviz pH, temperature and concentration of α-methyl glucoside influenced the binding and elution of these enzymes. A lysosomal
acid α-mannosidase and a cytosolic neutral ma...
Citations
... The ''cytosolic'' neutral a-mannosidase, referred to as Man2C1, occurs in various animal sources [152][153][154][155][156][157][158][159][160][161][162][163][164][165]. It has been shown that the knockout of Man2C1 in mice results in a dramatic accumulation of Gn1 type of FNGs with Man 8-9 (Man 8-9 GlcNAc 1 ) in the cytosol of all tissues tested [166], strongly indicating that the action of Man2C1 is responsible for generating M5B 0 in the cytosol (Fig. 3). ...
... The ''cytosolic'' neutral a-mannosidase, referred to as Man2C1, occurs in various animal sources [152][153][154][155][156][157][158][159][160][161][162][163][164][165]. It has been shown that the knockout of Man2C1 in mice results in a dramatic accumulation of Gn1 type of FNGs with Man 8-9 (Man 8-9 GlcNAc 1 ) in the cytosol of all tissues tested [166], strongly indicating that the action of Man2C1 is responsible for generating M5B 0 in the cytosol (Fig. 3). ...
