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The article presents results obtained from monitoring chemical and ecological state of ambient water ecosystem, especially the surface water quality with the focus on radiological analysis and assessment of ecotoxicity and genotoxicity, executed on the Hadůvka stream, the main stream impacted by former uranium mining in the Olší mine area, during the period 2003 - 2008. Moreover, sediment and suspended particulate matter were tested for a number of parameters. The water quality research performed in the years 2003-2008 demonstrated an impact of the mine water pumped from the closed Olší uranium mine and discharged from the mine water treatment plant (MWTP) and groundwater from springs in the area on the water quality of the Hadůvka stream. The water ecosystems of the lower part of the Hadůvka stream are impacted mainly by water originated from the springs located in the stream valley and drained syenit subsoil naturally rich in uranium. These inflows caused a very high concentration of uranium measured in the water of the stream, which exceeds the given limit value. No negative impact on the water ecosystems of the receiving Bobrůvka River was found. This reduction of impact is caused by five times higher average daily flow rate of the Bobrůvka River in comparison with the Hadůvka stream, which results in sufficient dilution of pollution from the Hadůvka .