Qiao Zhang's scientific contributions

Publications (10)

Article
No gold standard currently exists for the diagnosis of general paresis (GP), thus often resulting in unnecessarily delayed therapeutic decision. A retrospective chart review was performed for 85 inpatients with GP in Zhongshan Hospital, Medical College of Xiamen University, and the characteristics of their clinical profiles, serum and cerebrospinal...
Article
We developed a new BOSON chemiluminescence immunoassay (CIA) and evaluated its application with cross-sectional analyses. Our results indicated that BOSON CIA demonstrated strong discriminatory power in diagnosing syphilis, and it can be used as a first-line screening test for syphilis serodiagnosis using the European Centre for Disease Prevention...
Article
Background: The prozone phenomenon is known to be associated with high antibody titers; other associations, such as host factors, have not been elucidated. Methods: A retrospective analysis was conducted to evaluate the incidence of the prozone phenomenon of the syphilis rapid plasma reagin (RPR) test among 46 856 clinical samples, between June...
Article
To characterize the CBFP reaction in the modern era, we analyzed the results of parallel rapid plasma reagin (RPR) and Treponema pallidum particle agglutination (TPPA) tests from a total of 63,765 blood samples obtained at Zhongshan Hospital in the Medical College of Xiamen University from May 2008 to February 2013. Among the 63,765 tested blood sa...
Article
Background: Algorithms for the diagnosis of syphilis continue to be a source of great controversy, and numerous test interpretations have perplexed many clinicians. Methods: We conducted a cross-sectional study of 24 124 subjects to analyze 3 syphilis testing algorithms: traditional algorithm, reverse algorithm, and the European Centre for Disea...
Article
Syphilis, a sexually transmitted disease, is commonly referred to as the "great imitator" because of its wide range clinical presentations. Recently, we notice that neurosyphilis (NS) patients seem to more easily lead to complication with diabetes mellitus (DM). It is an interesting phenomenon; however it also puzzles clinicians due to scanty knowl...
Article
Neurosyphilis (NS) caused by Treponema pallidum (T. pallidum) subspecies pallidum, can affect the central nervous system during any stage of the disease. To assess several laboratory parameters for NS diagnosis, we performed a case control study on 42 hospitalized NS patients negative for human immunodeficiency virus (HIV) and 40 syphilis/non-NS pa...

Citations

... With treponemal assays (e.g., EIA and CIA) gradually becoming available, these assays have allowed clinical laboratories to meet the increasing demands for syphilis screening, enhancing efficiency, and providing accurate results. Several automated commercially available treponemal CIAs including the Liaison CIA (Diasorin, Stillwater, MN, USA) (Knight et al., 2007) and the Architect syphilis CIA (Abbott, Wiesbaden, Germany) (Young et al., 2009), and treponemal EIAs such as Captia syphilis G, Captia syphilis M (Trinity Biotech, Ireland), and Spiro Tek syphilis test (Organon Teknika, USA) (Ratnam, 2005), have been used in clinical laboratories (Qiu et al., 2015). These commercial products demonstrated excellent sensitivity and specificity when evaluated as confirmatory test and as screening tests for syphilis in various patient populations. ...
... The results of blood culture for identification of pathogens were used as the gold standard in this study. The diagnostic performance of the PCR-MCA assay was determined and compared to the results of blood culture, using sensitivity, specificity, positive predictive value, negative predictive value (NPV), agreement rate and Kappa value, according to the Yerushalmy model (Niu et al., 2015). The sensitivity was calculated as the number of episodes in which both blood culture and PCR-MCA were positive divided by the sum of these episodes and the number of episodes in which blood culture was positive but PCR-MCA were negative. ...
... A wide use of SERODIA-TPPA ® (5 of 11 studies) was observed, differing in population data, CSF reactivity titulation and diagnostic accuracy test, summarized in Table 1. Two studies with SERODIA-TPPA that analyzed the population only without HIV used a titer of ≥ 1:80 and their statistical results were in agreement with CSF-VDRL and CSF-TRUST and total percentage of patients with reactive CSF-TPPA (19)(20). LI et al. use the same HIV population with a titer ≥ 1:320 and obtain a sensitivity of 84.09% and a specificity of 88.89%. ...
... 12 Historical infection was defined as TPPA positive but TRUST negative and active infection as both TPPA and TRUST positive. 14 Syphilis seropositivity was defined as TPPA positive, including both TRUST negative and TRUST positive. Moreover, high-titre was defined as a quantitative titre of ≥1:8 in patients with active infection. ...
... Biological false-positive results can occur in the context of pregnancy, autoimmune diseases or other infections (e.g., malaria, hepatitis C) [26], and NTT may be reactive in areas where yaws, pinta or non-venereal disease is endemic [27]. A RPR test can return a false negative result in early primary, late latent or tertiary syphilis [28], but a false-negative RPR test can also be found in primary or secondary syphilis with very high antibody titre (prozone phenomenon) [29]. As syphilis reinfection is associated with attenuated immunological responses and reduced antibody levels [30], the sensitivity of NTT decreases and test results may not be reliable for diagnosis of repeat syphilis [31]. ...
... In contrast, nontreponemal antibodies recognize the cardiolipin antigen and detect a mixture of phosphatidylcholine, cholesterol and cardiolipin, as the major antigenic component [4]. Antibodies to phospholipids are found in the sera of patients with infectious diseases, such as syphilis, tuberculosis and malaria, and autoimmune disorders, including systemic lupus erythematosus, rheumatoid arthritis and thrombocytopenic purpura [5,6]. Phospholipid antibody includes antibodies directed against the cardiolipin moiety of the Venereal Disease Research Laboratory (VDRL) antigen, antibodies reacting with the phospholipids of the prothrombin activator complex and antibodies reacting with solid phase cardiolipin [7]. ...
... Syphilis, a chronic multistage disease punctuated by asymptomatic periods of latency, is caused by the spirochete Treponema pallidum subsp. pallidum (hereafter T. pallidum) and is primarily transmitted sexually or vertically during pregnancy (1). Syphilis is clinically manifested when spirochetes replicating at the site of inoculation induce a local inflammatory response sufficient to generate a papule that subsequently ulcerates, forming a chancre; chancres are the defining lesions of primary syphilis and are typically painless and resolve spontaneously (2). ...
... Th ere have not existed recent studies investigating these phenomena. Th ere are some studies depicted there is a relationship between diabetes mellitus and syphilis however they have been reported in 1944, 1892, and 1917.26 A recent study has reported an association between neurosyphilis and diabetes mellitus.26 ...
... Therefore, early diagnosis and treatment of neurosyphilis is of great significance for regression [4]. Because of the complex stages of occurrence, changing clinical symptoms and variable laboratory indicators of neurosyphilis, early diagnosis of neurosyphilis is difficult [5][6][7]. ...