Qianying Li’s research while affiliated with South China Agricultural University and other places

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Publications (1)

Symptoms of Citrus limon infected with 200 female nematodes of Radopholus similis per plant for 30 days. CK: non-inoculated rough lemon seedling and root system; XIN, ML, SWK, HN6, HL, GJ, FZ, GZ, YJ and DBSR: the rough lemon seedlings and root systems were inoculated with the different populations of R. similis, which originated from Zingiber officinale Roscoe (XIN), Crataegus pinnatifida (ML), Chrysalidocarpus lutescens (SWK), Musa AAA Giant Cavendish cv.Baxi (HN6), Maranta arundinacea (HL), Citrus reticulata (GJ), Anthurium andraeanum ‘Pink Champion’ (FZ), Anthurium andraeanum Linden (GZ), Curcuma longa (YJ), and Anubias nana (DBSR), respectively
Tropism of 10 populations of Radopholus similis to the Citrus limon root. A: Tropism of R. similis to the root of C. limon in Pluronic F-127 gel evenly mixed with 200 female nematodes 6 h after assay initiation; B: Fuchsin staining of C. limon roots 8 h after assay initiation by infection with 200 female nematodes; XIN, ML, SWK, HN6, HL, GJ, FZ, GZ, YJ, and DBSR: the C. limon roots were infected with populations of R. similis, which originated from Zingiber officinale Roscoe (XIN), Crataegus pinnatifida (ML), Musa AAA Giant Cavendish cv.Baxi (HN6), Maranta arundinacea (HL), Chrysalidocarpus lutescens (SWK), Citrus reticulata (GJ), Anthurium andraeanum ‘Pink Champion’ (FZ), Anthurium andraeanum Linden (GZ), Curcuma longa (YJ), and Anubias nana (DBSR), respectively; scale bar = 500 μm
PCR amplification of five pectate lyase genes from Radopholus similis (Rs-pels). A: Amplification of the ORF sequences of Rs-pels; B: Amplification of the genomic DNA sequences of Rs-pels. M:DS2000 marker (GDSBio Co., Ltd., Guangzhou China); 1–5: products of Rs-pel-1, Rs-pel-2, Rs-pel-3, Rs-pel-4, Rs-pel-5 amplification respectively
Predicting the conserved regions of pectate lyases of the Class III PEL family from Radophulus similis. Regions I–IV indicate conserved regions characteristic of the Class III pectate lyase family (Shevchik et al.1997). Highly conserved charged residues are indicated with asterisks (*), RS_PEL2 to 5 indicate the amino acid sequences of Rs-PEL-2 to Rs-PEL-5 from R. similis, the species of the bacterium or the fungus, the gene, the amino acid size, and the accession number of the aligned sequences are: F_sol_PelA = Fusarium solani f. sp. pisi, PelA, 242 aa (M94692.1); F_sol_PelB = F. solani f. sp. pisi, PelB, 242 aa (U13051); F_sol_PelC = F. solani f. sp. pisi, PelC, 215 aa (U13049); F_sol_PelD = F. solani f. sp. pisi, PelD, 233 aa (U13050); E_car_PelB = Erwinia carotovora, PelB, 347 aa (X79232)
Maximum-likelihood phylogenetic trees of pectate lyases of Radopholus similis (Rs-PELs) and other organisms. Phylogenetic tree for proteins with conserved domains of pectate lyases from cyst nematodes, root-knot nematodes, Aphelenchus, Bursaphelenchus, bacteria, and fungi generated by MEGA6.0. The Rs-PELs amino acid sequences were marked in bold, and each sequence was followed by its accession number in GenBank

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Pectate lyase genes from Radopholus similis and their application in pathotype identification
  • Article
  • Full-text available

April 2024

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Applied Microbiology and Biotechnology

Sihua Yang

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Shuai Yang

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Qianying Li

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Radopholus similis is a destructive, migratory, and endophytoparasitic nematode. It has two morphologically indistinguishable pathotypes (or physiological races): banana and citrus pathotypes. At present, the only reliable method to differentiate the two pathotypes is testing the infestation and parasitism of nematodes on Citrus spp. via inoculation. However, differences in inoculation methods and conditions adopted by different researchers complicate obtaining consistent results. In this study, the parasitism and pathogenicity of 10 R. similis populations on rough lemon (Citrus limon) seedlings and the tropism and invasion of rough lemon roots were tested. It revealed that populations SWK, GJ, FZ, GZ, DBSR, and YJ were citrus pathotypes, which showed parasitism and pathogenicity on rough lemon and could invade rough lemon roots, whereas populations XIN, ML, HN6, and HL were banana pathotypes, having no parasitism and pathogenicity on rough lemon and they did not invade the rough lemon roots. Four pectate lyase genes (Rs-pel-2, Rs-pel-3, Rs-pel-4, and Rs-pel-5) belonging to the Class III family from these populations were amplified and analysed. The gene Rs-pel-3 could be amplified from six citrus pathotype populations and was stably expressed in the four developmental stages of the nematode, whereas it could not be amplified from the four banana pathotypes. Rs-pel-3 expression may be related to the parasitism and pathogenicity of R. similis on rough lemon. Hence, it can be used as a molecular marker to distinguish between banana and citrus pathotypes and as a target gene for the molecular identification of these two pathotypes. Key points • Four pectate lyase genes (Rs-pels) from Radopholus similis were cloned and analysed. • The expression of Rs-pels is different in two pathotypes of Radopholus similis. • A molecular identification method for two pathotypes of Radopholus similis using pectate lyase gene Rs-pel-3 as the target gene was established.

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