Puchang Lie's research while affiliated with Chinese Academy of Sciences and other places

Publications (16)

Article
The umbilical cord contains different populations of stem/progenitor cells such as mesenchymal, hematopoietic, trophoblastic and endothelial cells. However, the isolation methods currently in use are different, and the resulted cells are liable to cross contamination. Thus it is increasingly difficult to compare and contrast the study outcomes, whi...
Article
Full-text available
Umbilical cord mesenchymal stem cells (UC‑MSCs) have been suggested as a candidate for various clinical applications, however, major limitations include the lack of organ‑specific accumulation and low survival rates of transplanted cells. In the present study, it was hypothesized that the paracrine effects of UC‑MSCs may enhance stem cell‑based tis...
Article
Cell-based therapies are major focus of current research for treatment of liver diseases. In this study, mesenchymal stem cells were isolated from human umbilical cord Wharton's jelly (WJ-MSCs). Results confirmed that WJ-MSCs isolated in this study could express the typical MSC-specific markers and be induced to differentiate into adipocytes, osteo...
Article
Full-text available
We demonstrated a new spectrophotometric DNA detection approach based on a circular strand-displacement polymerization reaction for the quantitative detection of sequence specific DNA. In this assay, the hybridization of an immobilized hairpin probe on the microtiter plate, to target DNA, results in a conformational change and leads to a stem separ...
Article
Full-text available
The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal...
Article
A rapid and reliable screening test for thalassemia carrier couples is the most effective strategy to decrease the risk of conceiving fetuses with severe thalassemia. We present an approach based on the isothermal strand-displacement polymerase reaction and the use of a lateral flow strip for the visual detection of an α-thalassemia Southeast Asian...
Article
Full-text available
A hairpin DNA probe mediated cascade signal amplification method was developed for visual and rapid DNA analysis with a detection limit of 100 aM. The implementation of tag/anti-tag DNA and gold nanoparticle reporters permits a universal platform for multiplex genotyping without instrumentation.
Article
Full-text available
A double-stranded DNA (dsDNA) mediated sandwich assay was developed for quantitative detection of transcription factors. The detection limit for human recombinant c-jun protein is 2.5 ng, and for c-jun protein the limit is as low as 0.625 μg of cell lysate.
Article
Full-text available
A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.
Article
The first example of strip logic gates ("OR" and "AND" functions) for proteins and small molecules has been constructed on the basis of target-induced self-assembly of split aptamer fragments. Using thrombin and ATP as inputs, the corresponding split/integrated aptamers as molecular recognition elements, and gold nanoparticles as a tracer, the outp...
Article
Full-text available
A lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles has been developed for the visual detection of nucleic acids with a detection limit of 0.01 fM.
Article
A lateral flow biosensor based on an immuno-chromatographic assay has been developed for the detection of DNA-binding proteins. The biosensor is composed of four parts: a sample pad, a conjugate pad, a strip of nitrocellulose membrane and an absorbent pad. A DNA probe containing a specific protein binding consensus sequence is coated onto gold nano...
Article
Full-text available
A lateral flow nucleic acid biosensor based on copper-dependent DNA-cleaving DNAzyme and gold nanoparticles has been developed for the visual detection of copper ions (Cu(2+)) in an aqueous solution with a detection limit of 10 nM.

Citations

... PHBVHHx is considered a promising PHA member for the growth of stem cells, and certain studies utilized it as biomaterial for the preparation of three-dimensional supportive scaffolds for organ tissue. In some works [50,51], PHBVHHx films and scaffolds were developed and loaded with mesenchymal stem cells from human umbilical cord (UC-MSCs) to recover injured liver. Biopolymer scaffolds were transplanted into liver-injured mice, and the results demonstrated that the PHA scaffold significantly promoted the recovery of injured liver and could be used for liver tissue engineering. ...
... Conventional static cell culture used in investigating the paracrine function of BMSCs on endothelial cells have yielded a great deal of insights into underlying mechanisms governing angiogenesis [66][67][68] . However, these static cell cultures limitedly re-flect the situation at the fracture site, as fluid shear stress is crucial to accurately mimic the repair process. ...
... Preparation of streptavidin-coated magnetic microsphere beads Carboxylated magnetic microsphere beads (MBs) (25 mL [1% w/v]) were functionalized with streptavidin (SA) (25 mL, 1 mg/mL), according to a previously reported method with some modification. 4,11,12 Briefly, 1-Ethyl-3-(3dimethylaminopropyl)-carbodiimide (EDC) (20 mg/mL, 20 mL) and N-Hydroxysuccinimide (NHS) (10 mg/mL, 20 mL) were mixed with precooled 4-Morpholine Ethane Sulfonic Acid 9 (MES) buffer (25 mM, pH 5, 10 mL) and then slowly dripped into MBs solution and shaken gently for 30 minutes at room temperature to activate the carboxyl groups. After washing three times with MES buffer (25 mM, pH 5, 10 mL), SA was slowly added and shaken gently for another 30 minutes. ...
... However, LFNAB is a qualitative or semi-quantitative detection method and the color observation may vary between diferent people. Therefore, we have developed a spectrophotometric DNA detection approach based on CSDPR for sensitive, selective, inexpensive, and quantitative detection of South East Asia (SEA) type of α-thalassemia [12]. As depicted in Figure 4, the signal ampliication system is composed of a hairpin capture probe, a primer, polymerase, and streptavidin-horse radish peroxidase (SA-HRP). ...
... Critically, it has been used to detect several pathogens such as SARS-CoV-2, hepatitis B virus, and Streptococcus agalactiae (Cheng et al., 2020;Li et al., 2020;Chen et al., 2021). The gold nanoparticle-based lateral flow biosensor (LFB) is a paper-based platform, which facilitates a low-cost rapid diagnosis; it is robust, visual, sensitive, specific, and has a low limit of detection (LoD) (Zeng et al., 2013;Urusov et al., 2019). Importantly, the technique can be used in clinical settings to detect specific biomarkers, including antibodies, antigens, and DNA; therefore it is considered a new and easy-to-use POC device. ...
... Based on CSDPR and gold nanoparticles (AuNPs), we have developed a lateral low biosensor (LFB) for the visual detection of thalassemia genes [11]. This sensor is highly sensitive (the detection limit is 0.01 fM of nucleic acid), highly speciic, and easy to use. ...
... Various multiplex DNA/RNA detection techniques have been reported. [63][64][65][66][67][68][69][70][71] Some are based on colorimetric changes that could be readily viewed with the naked eye. 65 are based on fluorescence detection. ...
... In one study, LFB was developed to detect human pluripotent stem cells by forming a sandwich assay on a nitrocellulose membrane. The accumulation of antibody conjugated gold nanoparticles on the membrane created visual signals which resulted in detection of 10,000 stem cells [15]. In another study, CD45+ cells were tagged with antibody conjugated gold nanoparticles and trapped in the paper, colorimetric intensity measurements of gold nanoparticles resulted in counting 60,000-160,000 cells [14]. ...
... This LFB sensing approach has a lot of promise in terms of detecting genetic disorders and cancer mutations. It may also be used in individualized medicine [122,123]. ...
... In order to perform the digital computing at the molecular level, DNA molecule can be used as fundamental logic circuit elements to replace MOS transistors (Arter et al., 2020;Song et al., 2019;Su et al., 2019;Wang et al., 2020b). Through modular molecular design and programmable reaction dynamics, DNA logic circuits can realize the biocomputing with excellent scalability and good accuracy (Cai et al., 2022;Chen et al., 2012Chen et al., , 2018Chen et al., , 2021Feng et al., 2020;Gong et al., 2019;Zhang et al., 2020). However, there are some limitations in the expansion of the molecular circuit. ...