Priya Mohanty's research while affiliated with University at Buffalo, The State University of New York and other places

Publications (71)

Article
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We have recently shown that a high-fat high-carbohydrate (HFHC) meal induces an increase in plasma concentrations of endotoxin (lipopolysaccharide [LPS]) and the expression of Toll-like receptor-4 (TLR-4) and suppresser of cytokine signaling-3 (SOCS3) in mononuclear cells (MNCs) in addition to oxidative stress and cellular inflammation. Saturated f...
Article
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The intake of glucose or a high-fat, high-carbohydrate (HFHC) meal, but not orange juice, induces an increase in inflammation and oxidative stress in circulating mononuclear cells (MNCs) of normal-weight subjects. We investigated the effect of orange juice on HFHC meal-induced inflammation and oxidative stress and the expression of plasma endotoxin...
Article
Data demonstrate the anti-inflammatory effects of insulin and proinflammatory effects of glucose. These data provide a mechanistic justification for the benefits of maintaining euglycemia with insulin infusions in hospitalized patients. Regimens that infuse fixed doses of insulin with high rates of glucose are usually associated with hyperglycemia,...
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Recent work shows a high prevalence of low testosterone and inappropriately low LH and FSH concentrations in type 2 diabetes. This syndrome of hypogonadotrophic hypogonadism (HH) is associated with obesity, and other features of the metabolic syndrome (obesity and overweight, hypertension and hyperlipidemia) in patients with type 2 diabetes. Howeve...
Chapter
The concept that insulin affects carbohydrate, lipid, and protein metabolism and that its deficiency leads to diabetes mellitus has given this hormone a central position as a key metabolic regulator. However, its role as a vasoactive hormone and as an inhibitor of inflammation and oxidative stress has been revealed over the past decade and a half....
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Low-dose insulin infusion has been shown to exert a prompt and powerful anti-inflammatory effect. Toll-like receptors (TLRs) are major determinants of the inflammatory response to viral and bacterial pathogens. We have now hypothesized that low-dose insulin infusion in obese type 2 diabetic patients suppresses TLR expression. Ten type 2 diabetic pa...
Article
Acute hyperglycemia worsens morbidity and mortality in critically ill patients. The control of hyperglycemia with insulin improves clinical outcomes in patients with a stay of more than 3–5 days in the intensive care unit (ICU) and in coronary artery bypass graft (CABG) patients. However, clinical benefits of insulin infusion have not been seen con...
Article
Background The restoration of normoglycemia ensures the control of diabetic symptoms and reduction in microangiopathic complications in type 1 and type 2 diabetes. However, there is no conclusive evidence that intensive glycemic control alone will prevent macrovascular disease, the commonest cause of morbidity and mortality in type 2 diabetes. As a...
Article
Endothelial dysfunction, which leads to impaired vasodilation, is an early event in the development of atherosclerosis. A number of mechanisms involving, for example, cell adhesion molecules, chemokines, and cytokines, contribute to this inflammatory disease, and insulin resistance plays a cardinal role in accelerating these processes. Hyperglycemi...
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Because obesity is associated with chronic oxidative and inflammatory stress, and high-fat, high-carbohydrate meals induce significant oxidative and inflammatory stress in normal subjects, we have now hypothesized that the intake of a high-fat, high-carbohydrate meal would result in a greater and more prolonged oxidative and inflammatory stress in...
Article
Ischemia-induced flow-mediated vasodilatation (FMD) of the brachial artery has emerged as the standard test for endothelial function in humans in vivo (1). It has been established that the reestablishment and the increase in flow following a period of ischemia is due to the release of nitric oxide (NO) from the endothelium, which leads to vascular...
Article
To evaluate the effect of exenatide on clinical parameters in obese patients with type 2 diabetes mellitus whose hyperglycemia is not adequately controlled despite treatment with oral hypoglycemic agents and insulin. In this retrospective analysis, clinical progress of 52 obese patients with type 2 diabetes treated with exenatide, 5 mcg twice daily...
Article
This review deals with the recent observations on the pro-inflammatory effects of glucose and the anti-inflammatory actions of insulin. Apart from being novel, they are central to our understanding of why hyperglycemia is a prognosticator of bad clinical outcomes including patients with acute coronary syndromes, stroke and in patients in the intens...
Article
Previously published data clearly demonstrate that hyperglycemia worsens morbidity and mortality in patients in intensive care, those with acute myocardial infarction and stroke, and those undergoing coronary artery bypass grafting. The control of hyperglycemia with insulin infusion improves clinical outcomes in these patients. In this article, we...
Article
We have previously shown that 300 kcal from glucose intake induces a significant increase in reactive oxygen species (ROS) generation and nuclear factor-kappaB (NF-kappaB) binding in the circulating mononuclear cells in healthy normal subjects. We hypothesized that the intake of 300 calories as orange juice or fructose, the other major carbohydrate...
Article
Previously published data demonstrate clearly that hyperglycemia worsens morbidity and mortality in patients in intensive care, those with acute myocardial infarction and stroke, and those who undergo coronary artery bypass grafting. The control of hyperglycemia with insulin infusion improves clinical outcomes in all these classes of patients. In t...
Chapter
The common occurrence of the combination of obesity, insulin resistance, hypertension, hypertriglyceridemia, low HDL cholesterol, and hyperinsulinemia was first described by Reaven as insulin resistance syndrome or metabolic syndrome. The syndrome was recognized as a pro-atherogenic risk causing coronary heart disease (CHD). More recently, other fe...
Article
Previously published data demonstrate clearly that hyperglycemia worsens morbidity and mortality in patients in intensive care, those with acute myocardial infarction and stroke, and those undergoing coronary artery bypass grafts. The control of hyperglycemia with insulin infusion improves clinical outcomes in all classes of patients mentioned abov...
Article
Because hyperglycemia is a major detrimental factor in the prognosis of acute cardiovascular conditions such as acute myocardial infarction (AMI) and stroke, and because an acute glucose challenge in healthy subjects has been shown to induce oxidative stress in mononuclear cells (MNCs), we have now investigated whether glucose induces inflammatory...
Article
Metabolic syndrome defined by a cluster of clinical features increases the risk of cardiovascular disease and type 2 diabetes mellitus. The original hypothesis linking this syndrome to these diseases was based on insulin resistance and the effect of hyperinsulinemia on atherogenesis. However, inflammatory processes have now been linked to the patho...
Article
Hyperglycemia worsens morbidity and mortality for patients in intensive care or with acute myocardial infarction, stroke, or coronary artery bypass grafts. The control of hyperglycemia with insulin improves clinical outcomes for patients with these conditions. This article reviews the anti-inflammatory effects of insulin and pro-inflammatory effect...
Article
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The discovery of the antiinflammatory effect of insulin and the proinflammatory effect of glucose has not only provided novel insight into the mechanisms underlying several disease states but has also provided a rationale for the treatment of hyperglycemia in several acute clinical conditions. Van den Berghe et al. previously showed the benefits of...
Article
he original description of the metabolic syndrome by Reaven 1 consisted of obesity, insulin resistance, hyper- tension, impaired glucose tolerance or diabetes, hyperinsulin- emia and dyslipidemia characterized by elevated triglyceride, and low HDL concentrations. All of the features described above are risk factors for atherosclerosis, and thus, me...
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The objective of the study was to determine whether plasma migration inhibitor factor (MIF) concentration and mononuclear cell (MNC) mRNA are elevated in obesity and whether treatment with metformin reduces plasma MIF concentration. Forty obese subjects [body mass index (BMI), 37.5 +/- 4.9 kg/m(2)] and 40 nonobese healthy subjects (BMI, 22.6 +/- 3....
Article
In view of the increase in plasma concentrations of proinflammatory mediators tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and C-reactive protein (CRP) in obesity, we investigated whether peripheral blood mononuclear cells (MNC) from obese subjects are in a proinflammatory state. MNC were prepared from fasting blood samples of obe...
Article
Glucose intake has been shown to cause an increase in intranuclear nuclear factor-kappa B and a decrease in inhibitor kappa B that are consistent with a proinflammatory effect. We investigated the effect of glucose intake on 2 other proinflammatory transcription factors, activator protein 1 (AP-1) and early growth response 1 (Egr-1), and on the gen...
Article
We have recently demonstrated a potent antiinflammatory effect of troglitazone, an agonist of peroxisome proliferates activated receptor γ (PPARγ) and a partial agonist of PPARα in both the nondiabetic obese and diabetic obese subjects. We have now investigated the antiinflammatory actions of rosiglitazone, a selective PPARγ agonist. Eleven nondiab...
Article
In view of the stimulatory effect of glucose on reactive oxygen species (ROS) generation, we investigated the possibility that a mixed meal stimulates ROS generation and possibly induces concomitant proinflammatory changes. The objective was to determine whether the intake of a 900-kcal mixed meal induces an increase in ROS generation by leukocytes...
Article
It has previously been shown that oral intake of 300 calories of glucose (75 g), lipid, or protein increases reactive oxygen species (ROS) generation by polymorphonuclear cells (PMNL) and mononuclear cells (MNCs). We investigated the effects of 75 g glucose on proinflammatory transcription factor, nuclear factor-kappaB (NFkappaB), in mononuclear ce...
Article
The metabolic syndrome, as described by Reaven, is a combination of obesity, insulin resistance, hypertension, hypertriglyceridemia, low HDL cholesterol, and hyperinsulinemia. The syndrome was recognized as a very high pro-atherogenic risk causing coronary heart disease. More recently, other features like an elevated plasma PAI -1 and CRP have been...
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To test the possible acute proinflammatory effects of fatty acids, we induced an increase in plasma free fatty acid (FFA) concentrations after a lipid and heparin infusion for 4 h in 10 healthy subjects. We determined the nuclear factor-kappaB (NF-kappaB) binding activity in mononuclear cells (MNCs), the p65 subunit of NF-kappaB, reactive oxygen sp...
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We recently demonstrated a potent anti-inflammatory and thus a potential antiatherogenic effect of insulin in human aortic endothelial cells and mononuclear cells at physiologically relevant concentrations. We have now further investigated the anti-inflammatory suppressive action of insulin on vascular endothelial growth factor (VEGF) and matrix me...
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In view of the pro-oxidant and proinflammatory effects of angiotensin II, we have tested the hypothesis that valsartan, an angiotensin receptor blocker, may exert a suppressive action on reactive oxygen species (ROS) generation, nuclear factor kappa B (NF-kappa B) in mononuclear cells. Four groups of eight normal subjects were given 1) 160 mg daily...
Article
Impaired cerebrovascular reserve could contribute to the increased risk of strokes in type 2 diabetes. We have shown a vasodilatory effect of insulin on the internal carotid artery in healthy subjects. As absence of this effect could be responsible for the impairment of cerebral blood flow reserve demonstrated in this population, we have now invest...
Article
It was recently shown that glucose challenge leads to increased generation of reactive oxygen species (ROS) by polymorphonuclear leukocytes (PMNs) and mononuclear cells (MNCs). To further elucidate the relation between nutrition and ROS generation, we investigated the effect of lipid and protein challenges on ROS generation by leukocytes. After hav...
Article
The purpose of this study was to assess the response of diabetics to scaling and root planing treatment and subgingival oral irrigation as adjunctive therapy. A total of 52 type 1 and 2 diabetics (mean age 51.3+/-14) with adult periodontitis were randomized to two groups. Treatment included ultrasonic scaling and scaling and root planing in both gr...
Article
We have recently demonstrated that an infusion of a low dose of insulin reduces the intranuclear NF-kappa B (a pro-inflammatory transcription factor) content in MNC while also reducing the plasma concentration of NF-kappa B dependent pro-inflammatory cytokines and adhesion molecules. We have now tested the effect of insulin on the pro-inflammatory...
Article
Having demonstrated recently that hydrocortisone (HC) suppresses intranuclear and total cellular nuclear factor-kappa B (NF-kappa B) and increases inhibitor kappa B (I kappa B) in mononuclear cells (MNC), in vivo, we have now investigated the effect of hydrocortisone on the other major pro-inflammatory transcription factor, AP-1 and the two protein...
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In view of the fact that insulin resistance is associated with atherogenesis and that troglitazone, an insulin sensitizer, has anti-inflammatory effects, which may be potentially antiatherogenic in the long term, we have now investigated whether insulin has potential anti-inflammatory effects. We infused 2.0 to 2.5 IU/h in 5% dextrose (100 mL/h) iv...
Article
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It has been shown recently that troglitazone exerts an anti- inflammatory effect, in vitro, and in experimental animals. To test these properties in humans, we investigated the effect of troglitazone on the proinflammatory transcription factor nuclear factor-kB and its inhibitory protein IkB in mononuclear cells (MNC) and plasma sol- uble intracell...
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We have recently demonstrated that troglitazone exerts an anti-inflammatory effect in the insulin resistant obese in vivo in parallel with its insulin-sensitizing effect. Because these effects are thought to be mediated through peroxisome proliferator-activated receptors alpha and gamma (PPARalpha and PPARgamma), we have now examined the possibilit...
Article
We have recently demonstrated that reactive oxygen species (ROS) generation by polymorphonuclear leukocytes (PMNL) and mononuclear cells (MNC) is inhibited following the intravenous administration of hydrocortisone. This is associated with a parallel decrease in intranuclear NFkappaB, known to modulate inflammatory responses including ROS generatio...
Article
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ABSTRACT Since glucose intake acutely increases reactive oxygen species (ROS) generation by polymorphonuclear leucocytes (PMN) and mononuclear cells (MNC), we have now investigated whether a fast over a period of 48h reduces ROS generation by these cells. Eight normal subjects were fasted for 48h. Blood samples were obtained at 0, 24h and 48h. ROS...
Article
We have recently demonstrated that reactive oxygen species (ROS) generation by polymorphonuclear leukocytes (PMNL) and mononuclear cells (MNC) is inhibited following the intravenous administration of hydrocortisone. This is associated with a parallel decrease in intranuclear NFκB, known to modulate inflammatory responses including ROS generation. W...
Article
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To elucidate whether troglitazone exerts an antiinflammatory ef- fect in humans, in vivo, we investigated the suppression of nuclear factor kB (NFkB) in mononuclear cells (MNC) by this drug. We mea- sured intranuclear NFkB, total cellular NFkB, inhibitor k B( I kB)a, reactive oxygen species (ROS) generation, and p47phox subunit (a key component pro...
Article
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Increased reactive oxygen species generation by the leukocytes of the obese may be responsible for increased oxidative injury to lipids and proteins and, hence, atherosclerosis. We have investigated whether reactive oxygen species generation by leukocytes and other indexes of oxidative damage in the body fall with short-term dietary restriction and...
Article
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Diabetes mellitus is associated with increased ROS generation, oxidative injury and obesity. To elucidate the relationship between nutrition and ROS generation, we have investigated the effect of glucose challenge on ROS generation by leucocytes, p47phox protein, a key protein in the enzyme NADPH oxidase and alpha-tocopherol levels. Blood samples w...
Article
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Because troglitazone has been shown to have antioxidant properties, we investigated whether troglitazone administration to obese subjects causes a reduction in (1) reactive oxygen species (ROS) generation by polymorphonuclear leukocytes (PMNLs) and mononuclear cells (MNCs) and (2) lipid peroxidation as reflected in the plasma concentrations of 9-hy...
Article
The increase in leg and foream blood flow induced by insulin could be secondary to its metabolic effect on glucose uptake. We therefore investigated whether insulin causes vasodilation of the internal carotid artery, since the brain is not dependent on insulin for glucose uptake, to demonstrate the the vasodilatory effect of insulin is primary and...
Article
We have recently demonstrated that hydrocortisone and other glucocorticoids inhibit reactive oxygen species (ROS) generation by mononuclear (MNC) and polymorphonuclear leucocytes (PMNL). Since NF-kappaB/IkappaB system regulates the transcription of proinflammatory genes, including those responsible for ROS generation, we tested the hypothesis that...
Article
After the demonstration that hydrocortisone inhibits reactive oxygen species (ROS) generation by leukocytes in vivo in a highly predictable manner, we investigated the effect of dexamethasone at a dose of 4 mg, which is thought to be roughly equivalent to 100 mg hydrocortisone. We also tested the hypothesis that dexamethasone may increase the plasm...
Article
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In view of the fact that glucocorticoids have an immunosuppressive effect and the fact that interleukin-10 (IL-10) is inhibitory to T helper cell function, we have now investigated the effect of hydrocortisone on plasma IL-10 concentrations. Seven normal subjects were injected with 100 mg hydrocortisone intravenously between 8 and 9:00 a.m. Sequent...
Article
The incidence of cardiovascular disease is lower in premenopausal women compared with men; following menopause, the risk of mortality from cardiovascular disease increases in females. Postischemic dilatation of the brachial artery has been used previously as an index of endothelium-mediated vasodilation. Using this index, we examined a group of pre...
Article
To assess whether, in the treatment of non-insulin-dependent diabetes mellitus (NIDDM), (1) metformin in conjunction with insulin can safely cause a decrease in glycosylated hemoglobin (HbA1c) to 7% or less and (2) this combination therapy may result in weight loss and lower insulin dose in comparison with insulin treatment alone. Forty patients wi...

Citations

... These findings are partly in line with data from previous epidemiological studies in Greek population [44], in which diets with a high intake of both protein and carbohydrates were associated with higher healthy aging markers, whereas diets only high in carbohydrates were linked with lower healthy aging scores among islander participants. These associations can be partly attributed to the fact that high carbohydrate intake results in an increase of insulin secretion, which in turn increases the risk of systematic inflammation [45]. On the other hand, proteins serve as more than just an energy resource for the human body, and higher protein intake has been associated with better physical performance in older adults [46]. ...
... It was previously shown that in prediabetics, troglitazone reduced plasma concentrations of hsCRP 39 and rosiglitazone suppressed the generation of reactive oxygen species by mononuclear cells ex vivo and reduced plasma hsCRP and MCP-1. 40 In both studies only nondiabetic obese subjects (7 for troglitazone and 11 for rosiglitazone) and no healthy lean controls were included. More recently, other studies have also shown that rosiglitazone decreased markers of inflammation and endothelial activation (CRP, PAI-1 and von Willebrand factor) in nondiabetic subjects with stable coronary artery disease, 41 hypertension, 42 or metabolic syndrome. ...
... Ceriello (2007) reviewed the anti-inflammatory activity of TZDs and found that these compounds act by modulating the anti-inflammatory markers such as IL-6, IL-18, CRP, MMP-2, MMP-9. Mohanty et al. (2004) evidenced the anti-inflammatory effect of rosiglitazone as a drug of the thiazolidinedione class that exerts an anti-inflammatory effect at the cellular and molecular level, and in plasma. In addition to these useful properties of thiazolidinedione products; furan and thiophene derivatives form a class of compounds with a broad spectrum of biological properties such as antibacterial (Holla et al., 1987), anti-allergic, antidepressant, antidiabetic, analgesic and anti-inflammatory activities (Mishra et al., 2011). ...
... Elsewhere in the body, T2D commonly presents with complications of fibrosis, retinopathy, neuropathy and cardiovascular disease, and these have multifactorial causes (Daryabor et al., 2020). T2D is a pro-inflammatory condition (Al-Shukaili et al., 2013;Tsalamandris et al., 2019) and peripheral vascular changes in patients with the disease are associated with altered immunological responses, increased TNFα, IL6, IL1β and IL17 (Berbudi et al., 2020;Dandona et al., 2007;Hang et al., 2014). Furthermore, T2D is associated with decreased immunosuppressive regulatory T-cell (Treg) activity and increased pro-inflammatory cytokines posing a greater risk of infection (Qiao et al., 2016;Samuel et al., 2019). ...
... Initial data have shown the differences in the composition of the adult intestinal microbiota among those with diabetes mellitus and control subjects (Larsen et al., 2010; Qin et al., 2012) and suggest that the composition of intestinal microbiota may influence the energy extraction of ingested foods, mucosal immunity, intestinal permeability and transit-time and systemic inflammation (Backhed et al., 2004; Cani and Delzenne, 2007; Gravitz, 2012). These factors have also been highlighted as triggers in the development and progression of T2DM and its complications (Ceriello and Motz, 2004; Dandona et al., 2004; Larsen et al., 2010; Stephens et al., 2009). Interestingly, administration of probiotics and prebiotics has been reported to be one of the most widely used approaches to modulate intestinal microbiota and may subsequently prevent or delay diabetes incidence (Steer et al., 2000). ...
... 13,14 Another research found that insulin infusion inhibited monocyte chemoattractant protein 1 (MCP-1) and soluble intercellular adhesion molecule 1 (sICAM-1) in obese nondiabetic patients. 15,16 However, the types of inflammatory cytokines analyzed in CSII treatment were limited at present, mainly including IL-1, IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, TNF-α, MCP-1, regulated upon activation normal T-cell expressed and secreted (RANTES), eotaxin, C-X3-C motif chemokine ligand 1 (CX3CL1), ICAM-1, macrophage migration inhibitory factor (MIF), tissue inhibitor of metalloproteinase-1 (TIMP-1), and tumor necrosis factor receptor type I (TNF-RI). The changes of other cytokines after CSII treatment were not clear. ...
... We have recently also demonstrated that troglitazone, a thiazolidinedione, known to be an insulin sensitizer, also causes an inhibition of ROS generation, an inhibition of NFB, an induction of IB and a suppression of p47 phox subunit (18,19). These changes are associated with a fall in oxidative damage and an improvement in vascular reactivity: postischemic vasodilatation of the brachial artery (18). ...
... Troglitazone is 37, a thiazolidinediones antidiabetic drug, used for people with diabetes mellitus type 2 ( Fig. 7) [18]. Rosiglitazone 38 is a thiazolidinedione antidiabetic drug. ...
... Type 2 DM is characterized by low-grade, chronic inflammation and an increased activity of IkB/NFkB seems to provide a molecular mechanism responsible for inflammation and insulin resistance in type 2 DM, associated with increased risk of cardiovascular diseases [30]. In endothelial cells, excess free fatty acids activate the pro-inflammatory IKKβ-NF-κB pathway causing cellular insulin resistance and impaired NO production [31]. ...