Pilar Martínez-Hidalgo's research while affiliated with King Juan Carlos University and other places

... For several decades, rhizobia have been recognized as the only inhabitants of legume nodules and biases in culture techniques prolonged this belief (Martinez-Hidalgo and Hirsch, 2017;Berza et al., 2021). Nevertheless, molecular advances and scientific progress achieved towards understanding legume-microbe interactions have revealed the co-existence of diverse bacterial community inside nodules composed of both symbionts and endophytes (Lyer and Rajkumar, 2017;Martínez-Hidalgo et al., 2022). ...

... have not been reported as phyllosphere endophytes, nonetheless, Lysinibacillus spp. have previously been isolated from tomato roots (Zhu et al., 2021), rice roots (Khaskheli et al., 2020;Shabanamol et al., 2021); and Peribacillus spp. was previously isolated from P. harmala and canola crop roots (Martínez-Hidalgo et al., 2021), making our study one of the few to isolate and report Peribacillus sp. as an endophyte. The 16S rRNA approach remains the gold standard for the initial identification of bacterial species, however, this approach cannot differentiate closely related bacterial species as is indicated by the formation of polytomy relationships in Figures 1-3 (Supplementary Figures 1-3; Kitahara and Miyazaki, 2013). ...

... Indeed, it has been demonstrated that Z. dumosum rhizosphere is associated with bacteria that were shown to confer stress protection [19]. Specifically, the bacterial isolate, Dietzia cinnamea, was shown to augment desiccation tolerance and significantly enhance the growth and yield of corn and wheat in lab and field trials [22,23]. Recent studies addressing the occurrence of desert endophytes in above-ground tissues revealed phylogenetically diverse communities dominated by Proteobacteria, Actinobacteria, and Firmicutes [24][25][26]. ...

... As aforementioned, biodegradation can be initiated in the presence of microbial communities such as bacteria, algae and fungi. However, the publication counts reported in González-Benítez et al. [371] indicated that bacterial remediation studies dominated the library of publications related to PPCPs biodegradation, followed by fungi-based PPCPs removal, while algal remediation studies constitute the minimal fraction of reported studies. ...

... Bioremediation encourages the reestablishment of plants on contaminated sites, which is comparatively less expensive and more suitable than chemical or physical remediation methods (Ullah, Heng, et al., 2015). The selection of microbes for bioremediation can be done either from similar contaminant sites or by preadaptation of bacteria under laboratory conditions (Molina et al., 2019). ...

... Many genome sequences belonged to the genera Myxococcus, Corallococcus, and Pyxidicoccus available in public databases provide an ideal opportunity for determining the threshold of a single molecular marker for species delineation. In comparison with other genetic markers, the gyrB gene with the much higher resolution has been fairly frequently used for determining phylogenetic relationships of closely related strains at the species level (Poirier et al., 2018;Martínez-Hidalgo et al., 2020;Klemetsen et al., 2021) and has been produced numerous reference sequences in public databases. Inspired by these results, we thus choose the gyrB gene as the candidate housekeeping gene. ...

... These authors reported that both the culture filtrates of B. subtilis and T. harzianum significantly inhibited the mycelial growth of the pathogen (Jangir et al., 2021). Khan et al. (2018) noted that 100 μg/mL of 1-butanol extraction of B. subtilis 30VD-1 hinders the 40% round spreading rate of Fusarium spp., while bacteria-infected pea seed treated with 30VD-1 showed a drop-down of wilt severity approximately 35% (Khan et al., 2018). One more in vivo application, B. subtilis V26 inhibited the development of the R. solani in potatoes and inhibited the development of the black curd disease and root canker by 81% and 63%, respectively (Ben Khedher et al., 2015). ...

... The primary organisms that maintain plant biomass production and plant health are specifically soil microorganisms [16] . Numerous researches have demonstrated that both direct and indirect modification of plant root and soil microbial populations appears to be a favorable technique to improve food crop productivity, nutritional quality, and safety with positive implications on human and environmental health [17,18] . Evidence suggests that soils with higher biodiversity levels are generally more resilient than those with lower biodiversity levels because they are more resilient to environmental perturbations [19] . ...

... N, which is absorbed from soil in the form of nitrate and ammonium, is essential for plant growth and development. The conversion of atmospheric N into ammonia takes place through different chemical processes, among which organic N fixation is the most important (Janczarek et al., 2015;Menéndez et al., 2017). Although N contributes the most to plant growth and development from an early stage, the N content decreased in the root and shoot tissue of both Mungbean cultivars under Cr stress levels, which, in turn, resulted in decreased plant growth and development, as well as decreased pod and seed yield per plant. ...

... Gram-negative microorganisms have been shown to be ineffective elicitors. Regarding the chitin composition of the cell wall, many treatments involving fungus and Gram-negative bacteria have been conducted [191]. The findings show that trans-resveratrol and transpiceatannol may be produced in controlled peanut culture using sterilized fungus and chitin as an efficient and quick elicitor that poses no threat from live bacteria. ...