Peter Serocka's research while affiliated with The Shanghai Institute of Technical Physics of the Chinese Academy of Sciences and other places

Publications (5)

Article
Full-text available
Unsupervised segmentation of multi-spectral images plays an important role in annotating infrared microscopic images and is an essential step in label-free spectral histopathology. In this context, diverse clustering approaches have been utilized and evaluated in order to achieve segmentations of Fourier Transform Infrared (FT-IR) microscopic image...
Article
Functional super-resolution (fSR) microscopy is based on the automated toponome imaging system (TIS). fSR-TIS provides insight into the myriad of different cellular functionalities by direct imaging of large subcellular protein networks in morphologically intact cells and tissues, referred to as the toponome. By cyclical fluorescence imaging of at...
Article
Full-text available
One of the problems arising when exploring toponome or other multivariate-image data is the following: Given a family of n gray-value images of, e.g., a given sample of cell tissue, indexed by a collection of n proteins under investigation (so-called MELK data) — each single image representing the varying local concentration of one of those n prote...
Conference Paper
A new challenge to data visualization has arisen from a new laboratory technique that is capable of imaging a large number of biomedical relevant molecule types in a single tissue probe, termed the Toponome. While aiming at deciphering the biochemical interactions of the molecules, and thus their biological functions as well their roles in diseases...
Article
Full-text available
We have implemented and investigated a spatial extension of the orig-inal ideal storage model by embedding it in a 2D cellular automaton with a diffusion-like coupling between neighboring cells. The resulting ideal storage cellular automaton model (ISCAM) generates many interesting spatio-temporal patterns, in particular spiral waves that grow and...

Citations

... Since the variables interact with each other in an unknown manner, the prediction of such a multivariable system based on limited time series data thus becomes a challenging task. We consider the data generated from an ideal storage cellular automaton model (ISCAM) simulating heterocatalytic reaction-diffusion processes at metal surfaces (45,46). The onestep prediction results by using the RDE framework for a spiral pattern in the 20 × 20 grids are illustrated in Fig. 5C, which clearly shows the effectiveness of our method for the spatiotemporal pattern prediction. ...
... Protein hydrolysates were produced from shrimp waste mainly comprising head and shell of Penaeus monodon by enzymatic hydrolysis for 90 min using four microbial proteases(Dey & Dora, 2014). Interractive similarity maps may identify more accurately the segmentations than the hierarchical clustering based approaches, and thus are a viable and due to their interactive nature attractive alternative to hierarchical clustering.As the former is much more efficient in time and memory, our results suggest another less resource demanding alternative for annotating large spectral images (Zhong et al., 2013). Enzymatic mungbean meal protein hydrolysate is a novel natural flavour/antioxidant source (Sonklin, Laohakunjit, Kerdchoechuen, & Ratanakhanokchai, 2018). ...
... A similar approach was proposed Humaun et al., 13 where phenotyping is carried out by unsupervised hierarchical clustering with a predefined number of 20 clusters. Affinity propagation clustering was also used by Kovacheva et al. 14 to phenotype colon cancer samples stained with 12 cell markers, while unsupervised K-SVD clustering was used by Spagnolo et al. 15 Dress et al. 16 introduce a series of metrics that can be used in TIS/multivariate images to quantify similarity between pixels and assist on clustering for cell phenotyping. These metrics were used in an interactive visualisation tool, where selecting a pixel automatically highlights all other pixels with similar protein distributions. ...
... By overlaying the interactive similarity maps (ISMs) with an H&E stained reference image, this allows to interactively take into account both spectral similarity and histopathological information from the stained image. This method is implemented in our so-called Lasagne software that has been originally proposed [13] and implemented [14] for multi-label fluorescence microscopy, while the present contribution adapts and quantitatively validates it for the use in vibrational microspectroscopy. ...
... It has been persuasively argued that many cellular functions are performed by highlevel complexes, modules or "supermolecules" [27,28]. Hence, those assemblies associated with viral processes can be targeted. ...