Pekka Palomäki's research while affiliated with Research Institute of the Finnish Economy, Finland, Helsinki and other places
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Publications (5)
New enzyme immunoassays (EIAs) for determination of specific IgG, IgA, and IgM antibody titers to Chlamydia pneumoniae were evaluated independently in three research laboratories. Specificity of the EIAs was enhanced by removing LPS from the chlamydial antigen. The performance of these EIAs was evaluated in comparison with the microimmunofluorescen...
Serology is commonly used for the diagnosis of acute Chlamydia pneumoniae infections and also for the diagnosis of complicated Chlamydia trachomatis infections. Furthermore, recent sero-epidemiological studies have linked C. pneumoniae infection with several diseases traditionally considered non-infectious. The objectives of this mini-review are to...
In the Toxoplasma gondii immunoglobulin M (IgM) capture fluorometric enzyme immunoassay used as a model, nonspecific responses due to the binding of human IgM to horseradish peroxidase (HRP) conjugates were observed despite the removal of the Fc portion of the immunoglobulin. This interaction may be mediated through the binding of human IgM to the...
A one-step third generation enzyme immunoassay (EIA) was developed for the detection of hepatitis B surface antigen (HBsAg) in human serum or plasma using a polyclonal (Pab-HBsAg) and two monoclonal antibodies to HBsAg (Mab1-HBsAg and Mab2-HBsAg). In this assay, the solid phase is coated with Mab1-HBsAg and the specimen is incubated simultaneously...
The application of 3-p-hydroxyphenylpropionic acid (HPPA), a fluorogenic substrate of horseradish peroxidase (HRP) to an automated microplate fluorometric enzyme immunoassay is described. Fluorescence intensity of the end product was highly dependent on the pH of the buffer and on the concentrations of the substrate mixture ingredients. The determi...
Citations
... Serology for C trachomatis (complement fixation titers >1:64) is usually performed infrequently, is nonstandardized, and requires a high level of expertise for interpretation. Furthermore, it may not perform as well as a test to diagnose rectal infections in males as in females with upper genital tract infections [45][46][47]. Testing for antigens requires invasive methods such as cervix swabs or urethral swabs. Compared to culture, this method has an 80-95% sensitivity. ...
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... In the 1990s EIA-based techniques emerged. Those employed treated EBs (59), or recombinant LOS antigens to detect genus-specific antibodies (60) or synthetic peptides from immunodominant MOMPs (61). Peptide-based immunoassay in diagnosis of C. trachomatis triggered ReA was found useful (62), however, the evidence of specific antibodies does not prove casualty. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272338268717073-1441941911881_Q64/Wim-Boersma.jpg)
... The hook effect is a major concern for one-step homogeneous immunoassays (32)(33)(34), including the Roche Elecsys HBsAg Quant II assay and the LICA. Excess HBsAg hinders the immunoassay by occupying the binding sites of the capture and detection antibodies simultaneously. ...
... The adaptation to HRP was attempted by Zapata and co-workers using 4-hydroxybenzoic acid (HBA) [5]. The oxidation products of 4-hydroxyphenylpropionic acid (pHPPA) [6], chavicol [7], Amplex red [8] and prochlorperazine [9] did not sensitize their fluorescence measurements in the corresponding experiment setup. The other common drawbacks of aforementioned fluorogenic substrates are their insufficient reaction rates, relatively low stability in air or toward H 2 O 2 in the absence of HRP, tending to cause strong background signals and poor water-solubility [10,11]. ...
... pH, DNA, zinc, copper and other metals determination (Purello et al., 1999). Among plant peroxidases, the most studied enzymes are native or recombinant horseradish peroxidases (HRP), widely used as enzyme labels in immunoassay kits (Shrivastav, 2003;Tuuminen et al., 1990;Tiirola et al., 2006), for organic synthesis, biotransformation of organic compounds, bioremediation of polluted waters (Veitch, 2004) and for construction of biosensors for H 2 O 2 detection (Tatsuma et al., 1998;Lindgren et al., 1999;Ferapontova et al., 2001). Roots of horseradish serve at present as the major source of commercially available peroxidase; however, the researchers still investigate for new peroxidases of elevated stability and properties suitable for different biotechnological processes. ...
