Pavel Voronin’s research while affiliated with Institute of Immunology and Physiology and other places

Rising atmospheric CO 2 (carbon dioxide) concentrations and salinization are manifestations of climate change that affect plant growth and productivity. Species with an intermediate C 3-C 4 type of photosynthesis live in a wide range of precipitation, temperature, and soil quality, but are more often found in warm and dry habitats. One of the intermediate C 3-C 4 photosynthetic type is C 2 photosynthesis with a carbon concentration mechanism (CCM) that reassimilates CO 2 released via photorespiration. However, the ecological significance under which C 2 photosynthesis has advantages over C 3 and C 4 plants remains largely unexplored. Salt tolerance and functioning of CCM were studied in plants from two populations (P1 and P2) of Sedobassia sedoides (Pall.) Freitag & G. Kadereit Asch. species with C 2 photosynthesis exposed to 4 d and 10 d salinity (200 mM NaCl) at ambient (785.7 mg/m 3 , aCO 2) and elevated (1571.4 mg/m 3 , eCO 2) CO 2. On the fourth day of salinity, an increase in Na + content, activity catalase, and superoxide dismutase was observed in both populations. P2 plants showed an increase in proline content and a decrease in photosynthetic enzyme content: rubisco, phosphoenolpyruvate carboxylase (PEPC), and glycine decarboxylase (GDC), which indicated a weakening of C 2 and C 4 characteristics under salinity. Treatment under 10 d salinity led to an increased Na + content and activity of cyclic electron flow around photosystem I (PSI CEF), a decreased content of K + and GDC in both populations. P1 plants showed greater salt tolerance, which was assessed by the degree of reduction in photosynthetic enzyme content, PSI CEF activity, and changes in relative growth rate (RGR). Differences between populations were evident under the combination of eCO 2 and salinity. Under long-term salinity and eCO 2 , more salt-tolerant P1 plants had a higher dry biomass (DW), which was positively correlated with PSI CEF activity. In less salt-tolerant P2 plants, DW correlated with transpiration and dark respiration. Thus, S. sedoides showed a high degree of photosynthetic plasticity under the influence of salinity and eCO 2 through strengthening (P1 plants) and weakening C 4 characteristics (P2 plants).

Abstract: Plant growth and productivity are predicted to be affected by rising CO 2 concentrations, drought and temperature stress. The C 3 crop model in a changing climate is Chenopodium quinoa Willd-a protein-rich pseudohalphyte (Amaranthaceae). Morphophysiological, biochemical and molecular genetic studies were performed on quinoa grown at ambient (400 ppm, aCO 2) and elevated (800 ppm, eCO 2) CO 2 concentrations, drought (D) and/or high temperature (eT) treatments. Among the single factors, drought caused the greatest stress response, inducing disturbances in the light and dark photosynthesis reactions (PSII, apparent photosynthesis) and increasing oxidative stress (MDA). Futhermore, compensation mechanisms played an important protective role against eT or eCO 2. The disruption of the PSII function was accompanied by the activation of the expression of PGR5, a gene of PSI cyclic electron transport (CET). Wherein under these conditions, the constant Rubisco content was maintained due to an increase in its biosynthesis, which was confirmed by the activation of rbcL gene expression. In addition, the combined stress treatments D+eT and eCO 2 +D+eT caused the greatest negative effect, as measured by increased oxidative stress, decreased water use efficiency, and the functioning of protective mechanisms, such as photorespiration and the activity of antioxidant enzymes. Furthermore, decreased PSII efficiency and increased non-photochemical quenching (NPQ) were not accompanied by the activation of protective mechanisms involving PSI CET. In summary, results show that the greatest stress experienced by C. quinoa plants was caused by drought and the combined stresses D+eT and eCO 2 +D+eT. Thus, drought consistently played a decisive role, leading to increased oxidative stress and a decrease in defense mechanism effectiveness.

Plant growth and productivity are predicted to be affected by rising CO2 concentrations, drought and temperature stress. The C3 crop model in a changing climate is Chenopodium quinoa Willd—a protein-rich pseudohalphyte (Amaranthaceae). Morphophysiological, biochemical and molecular genetic studies were performed on quinoa grown at ambient (400 ppm, aCO2) and elevated (800 ppm, eCO2) CO2 concentrations, drought (D) and/or high temperature (eT) treatments. Among the single factors, drought caused the greatest stress response, inducing disturbances in the light and dark photosynthesis reactions (PSII, apparent photosynthesis) and increasing oxidative stress (MDA). Futhermore, compensation mechanisms played an important protective role against eT or eCO2. The disruption of the PSII function was accompanied by the activation of the expression of PGR5, a gene of PSI cyclic electron transport (CET). Wherein under these conditions, the constant Rubisco content was maintained due to an increase in its biosynthesis, which was confirmed by the activation of rbcL gene expression. In addition, the combined stress treatments D+eT and eCO2+D+eT caused the greatest negative effect, as measured by increased oxidative stress, decreased water use efficiency, and the functioning of protective mechanisms, such as photorespiration and the activity of antioxidant enzymes. Furthermore, decreased PSII efficiency and increased non-photochemical quenching (NPQ) were not accompanied by the activation of protective mechanisms involving PSI CET. In summary, results show that the greatest stress experienced by C. quinoa plants was caused by drought and the combined stresses D+eT and eCO2+D+eT. Thus, drought consistently played a decisive role, leading to increased oxidative stress and a decrease in defense mechanism effectiveness.

The adaptation of plants to combined stresses requires unique responses capable of overcoming both the negative effects of each individual stress and their combination. Here, we studied the C3-C4 (C2) halophyte Sedobassia sedoides in response to elevated temperature (35 °C) and salinity (300 mM NaCl) as well as their combined effect. The responses we studied included changes in water–salt balance, light and dark photosynthetic reactions, the expression of photosynthetic genes, the activity of malate dehydrogenase complex enzymes, and the antioxidant system. Salt treatment led to altered water–salt balance, improved water use efficiency, and an increase in the abundance of key enzymes involved in intermediate C3-C4 photosynthesis (i.e., Rubisco and glycine decarboxylase). We also observed a possible increase in the activity of the C2 carbon-concentrating mechanism (CCM), which allowed plants to maintain high photosynthesis intensity and biomass accumulation. Elevated temperatures caused an imbalance in the dark and light reactions of photosynthesis, leading to stromal overreduction and the excessive generation of reactive oxygen species (ROS). In response, S. sedoides significantly activated a metabolic pathway for removing excess NADPH, the malate valve, which is catalyzed by NADP-MDH, without observable activation of the antioxidant system. The combined action of these two factors caused the activation of antioxidant defenses (i.e., increased activity of SOD and POX and upregulation of FDI), which led to a decrease in oxidative stress and helped restore the photosynthetic energy balance. Overall, improved PSII functioning and increased activity of PSI cyclic electron transport (CET) and C2 CCM led to an increase in the photosynthesis intensity of S. sedoides under the combined effect of salinity and elevated temperature relative to high temperature alone.