Paula Juricic's research while affiliated with Max Planck Institute for Biology of Ageing and other places

... Multiple drugs with anti-aging properties also link nutrient sensing to insulin action with potential ramification on immune function. Rapamycin, an anti-inflammatory drug inhibiting insulin-mTORC1 axis, has long been considered effective in extending lifespan (211), boosting immunity in the elderly (212), and delaying several age-related diseases (213)(214)(215)(216). The mechanism of this immunostimulatory effect of rapamycin are not well characterized, but could be linked to inhibition of mTORC1mediated S6 kinase negative feedback on age-associated chronic insulin signaling (5, 217). ...

... This possibility requires further study, as our analyses were limited and exploratory, and there are well-known concerns about metabolic side effects of 3TC in peripheral tissues (Max & Sherer, 2000;Thet & Siritientong, 2020). Indeed, our data suggest that 3TC may exacerbate the brain aging phenotype for certain metabolites (e.g., lleucine, which is thought to contribute to insulin resistance, a driver of brain aging, and metabolic dysfunction in rodents) (Solon-Biet et al., 2019), so this may be an important area for future work. ...

... These results suggest that lifespan extension is closely linked to TCA cycle and respiratory chain activity. In Drosophila, restriction of the essential amino acids methionine, threonine, histidine, lysine, or BCAAs extends lifespan (Lee et al., 2014;Juricic et al., 2019;Richardson et al., 2021). In rodents, dietary restriction of BCAAs increases mouse health and longevity (Richardson et al., 2021). ...

... 566acids, respectively(Piper et al. 2017). More importantly, while the latter two BCAA form a 567 similar percentages of amino acids in yeast, leucine content in yeast protein is only about 7.5% 568(Piper et al. 2017). ...

... It is, therefore, a principal constraint on evolutionary fitness [1,[4][5][6]. For example, the abundance of protein-rich food has been shown to increase population size or stimulate body growth of birds such as the galah (Eolophus roseicapilus) or the goldfinch (Carduelis carduelis) and mammals like the house mouse (Mus musculus) or several species of squirrels (Sciurus and Tamiasciurus spp.) [7][8][9][10][11][12][13]. In the fruit fly Drosophila melanogaster, we have found that female reproduction is reduced by decreasing overall dietary protein concentration [14][15][16]. We also found that this protein limitation is determined by the concentration of the single most limiting essential Amino Acid (AA) in the diet, which can be identified by comparing the proportion of AAs that is available in food against the proportion of AAs encoded by the fly's exome-a procedure we called exome matching [14][15][16]. ...