Patrícia Maria Guedes Paiva's research while affiliated with Federal University of Pernambuco and other places

Publications (230)

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Allied to the problem of gastrointestinal parasites, especially Haemochus contortus, the use of lectins of plant origin has contributed to the research of alternative anthelmintics. The nematicidal effect of a water soluble Moringa oleifera lectin (WSMoL) was investigated in an experimental model with H. contortus infected Wistar rodents. Three con...
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Currently, one of the main strategies to reduce the incidence of the arboviruses such as Zika, dengue, and chikungunya is through the population control of their vector, Aedes aegypti. Lectins are carbohydrate-binding proteins with several biological properties, including insecticidal activity against A. aegypti. Punica granatum sarcotesta contains...
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The present study aimed to evaluate saline extracts from the leaves (LE) and stem (SE) of Portulaca elatior in relation to their phytochemical composition and photoprotective and antioxidant effects, as well as to evaluate the toxicity of the leaf extract. The extracts were characterized for protein concentration and phenol and flavonoid contents,...
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Staphylococcus aureus is commonly found in wound infections where this pathogen impairs skin repair. The lectin isolated from leaves of Schinus terebinthifolius (named SteLL) has antimicrobial and antivirulence action against S. aureus. This study evaluated the effects of topical administration of SteLL on mice wounds infected by S. aureus. Seventy...
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The potential of plant lectins (carbohydrate-binding proteins) for the treatment of neurological disorders such as anxiety and depression has started to be reported in the last few years. Schinus terebinthifolia leaves contain a lectin called SteLL, which has displayed antimicrobial, immunomodulatory, antitumor, and analgesic activities. However, t...
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Species of the genus Trichophyton are etiological agents of dermatophytoses. The appearance of fungi resistant to drugs used to treat these diseases has prompted the search for natural compounds with anti-Trichophyton action. This study sought to determine the antifungal potential of protein fraction (PF) and fixed oil (FO) obtained from Moringa ol...
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Ethnopharmacological relevance: Preparations from the bark and leaves of Schinus terebinthifolia Raddi are commonly used to treat toothaches and sore throats. The use of medications based on leaves of this plant has also been reported for pain of arthritis, toothache, and sore throat. Some evidence indicated that the lectin SteLL is an antinocicep...
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There is a need to control populations of the termite Nasutitermes corniger and the mite Tetranychus bastosi since they act as urban and agricultural pests. Plant lectins (carbohydrate-binding proteins) have been suggested as a strategy for pest control. Thus, this study investigated the termiticidal and acaricidal activities of a mixture containin...
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Angiogenesis (budding of new blood vessels) is involved in several processes, including the development of embryos and growth of tumors. Schinus terebinthifolia leaves express an antitumor lectin (SteLL). This work hypothesized that SteLL can interfere with the formation of a vascular network from preexisting vessels. To test this hypothesis, the e...
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Ethnopharmacological relevance Eugenia uniflora Linn (Myrtaceae) is the native species of Brazil. The leaves of this species are used in folk medicine to treat different inflammatory and gastrointestinal disorders. However, research on the safety of using E. uniflora leaves has been poorly explored. Aim of the study: This approach aims to investiga...
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The present study compared the chromatographic profile of oils from P. corcovadensis leaves obtained through hydrodistillation, maceration, supercritical fluid, headspace, steam distillation and ultrasound to determine the best method for the obtainment of β-germacrene-D-4-ol. The oviposition deterrent and larvicidal activities of this compound wer...
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Flocculation has been proved an efficient method for microalgal biomass harvesting, but some coagulant agents may have adverse effects on microalgae growth, making the reuse of the medium unfeasible. In this study, Haematococcus pluvialis was harvested by different flocculants, and the feasibility of the reuse of the culture medium was evaluated. R...
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Ethnopharmacological relevance: Eugenia uniflora (Myrtaceae) is a species native to Brazil and has a traditional use in the treatment of inflammation. Aim of the study: To evaluate the anti-inflammatory and antinociceptive effects, and the involvement of opioid receptors in the antinociceptive activity of extract and fractions from Eugenia unifl...
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The traditional analysis of variance (ANOVA) of an experimental design is not efficient for evaluating the effect of all constituents simultaneously in gas chromatography mass spectrometry (GC–MS). This work evaluated the influence of temperature, pressure, extraction time and their interactions to obtain essential oil from Piper corcovadensis usin...
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Ethnopharmacological relevance Moringa oleifera Lam. leaves infusion and powder are widely used by population due the nutritional and medicinal potentials, however data regarding safety of use are still inconclusive, leading to prohibition of this plant in some countries. Aim of the study The present work investigated the nutritional and phytochem...
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Bio-flocculation is a sustainable low-cost harvesting technique for microalgae biomass production; however, it is generally less efficient than chemical flocculants. This study aims to investigate the efficiency of Moringa oleifera seeds as a bio-flocculant for harvesting Tetradesmus dimorphus biomass. Four extracts from integral and residual (seed...
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In recent years, lectins have been identified as alternative agents against Aedes aegypti during the aquatic phases of its life cycle. For example, chitin-binding lectin from Myracrodruon urundeuva leaf (MuLL) can function as a larvicide. In this study, we investigated whether MuLL can also act as an ovicide against this insect. Aedes aegypti eggs...
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Most anti-inflammatory drugs used nowadays have an excessive cost and their prolonged use has been connected with several injurious effects. Thus, the search for new anti-inflammatory agents is increasing. Lectins are carbohydrate-interacting proteins that can modulate immune response and the release of inflammation mediators. The Microgramma vacci...
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Since the dawn of mankind, plants figure among the main sources of therapeutic compounds, in addition to serving as inspiration for the production of synthetic or biotechnological-based drugs, including antimicrobial compounds. In the last 70 years, there has been a growing application of biochemical and molecular biology methods for the isolation...
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The control of fall armyworm (Spodoptera frugiperda) populations involves the application of pesticides that cause several unwanted effects to humans and other animals, as well as to environment. Sodium chloride (NaCl) has been used in agriculture to potentiate the toxicity of pesticides. Here, the effects of NaCl on the viability of eggs, larvae,...
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PgTeL is a chitin-binding lectin from Punica granatum sarcotesta and shows antimicrobial activity against a wide range of microorganisms of medical interest. PgTeL did not show cytotoxicity in human peripheral blood mononuclear cells; however, there have been no studies to date on PgTeL toxicity in vivo. The present study evaluated PgTeL for cytoto...
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This work aimed to purify, characterize, and evaluate the immunomodulatory activity of a lectin from Aesculus hippocastanum seeds (AhSL). The lectin was isolated from saline extract through chromatography on a DEAE-Sephadex column. Protein homogeneity and subunit composition were evaluated with polyacrylamide gel electrophoresis (PAGE) under native...
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The rhizome of Microgramma vacciniifolia contains a lectin (carbohydrate-binding protein) called MvRL. Studies demonstrated that a MvRL-rich fraction did not show in vivo genotoxicity and acute toxicity in mice. This study aimed to evaluate the MvRL-rich fraction from M. vacciniifolia rhizome for antibacterial activity in vitro and in vivo as well...
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This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3 ) larvae to the saline extract of Opuntia ficus-indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at ¼ LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50 ; the develo...
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The ethanolic extract from Croton blanchetianus leaves has been shown to have antinociceptive activity in mice. Here, we investigated the antinociceptive activity of an ethyl acetate fraction (EAF) from this extract in mice and the possible pathways involved in the analgesic effect. Adverse effects on behavior and motor coordination were also evalu...
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Croton blanchetianus Baill, commonly known as “marmeleiro”, is native to the Brazilian Caatinga is traditional used to treat joint pain and gastrointestinal disorders. The aim of the study was identify the chemical constituents by HPLC-DAD and determine the antioxidant activity of the spray-dried extract (SDE) aqueous (AqF) and ethyl acetate (EAF)...
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BACKGROUND Sitophilus zeamais is economically one of the most impactful pests, attacking various grains and processed foods. The control of this insect has been achieved using synthetic insecticides, whose exacerbated and careless use has led to the development of resistant insect populations, toxicity to non-target organisms, and environmental con...
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This study reports the development of conjugates based on quantum dots (QD)s and lectins from Schinus ter-ebinthifolia leaves (SteLL) and Punica granatum sarcotesta (PgTeL). Cryptococcus neoformans cells were chosen to evaluate the efficiency of the conjugates. Lectins were conjugated to QDs via adsorption, and the optical parameters (emission and...
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Moringa oleifera seeds are used globally as a treatment for water and contain the lectins cMoL and WSMoL, which display coagulant activity. In this study, we sought to determine the cytotoxicity and genotoxicity of the M. oleifera seed extract (SE), prepared with the same procedure that people use for treating water, as well as cMoL and WSMoL, in h...
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Aedes aegypti can transmit dengue, chikungunya, zika and yellow fever. Its control represents the main strategy for reducing the spreading of these diseases. In the present work, Plectranthus barbatus leaf saline extract (SE), decoction and infusion were studied for their effects on the survival of A. aegypti third instar larvae (L3), as well as fo...
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Lectins are carbohydrate-binding proteins with several bioactivities, including antimicrobial properties. Portulaca elatior is a species found at Brazilian Caatinga and data on the biochemical composition of this plant are scarce. The present work describes the purification of P. elatior leaf lectin (PeLL) as well as the assessment of its antimicro...
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The treatment of persistent pollutants in water is a subject of numerous researches around the world. In the present work, the degradation of a mixture of textile dyes by the photo-Fenton process was investigated. This was conducted under LED radiation, which has a reduced cost, employing iron in salt and residue forms. For homogeneous and heteroge...
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Lippia alba is an aromatic shrub known to produce a diversity of essential oils, which can be classified into chemotypes. This study reports on the insecticidal activity of essential oil from L. alba leaves collected at Caatinga and its major compound against termite Nasutitermes corniger and maize weevil Sitophilus zeamais. The chromatographic ana...
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Moringa oleifera, a plant widely used in traditional medicine as well as for water purification, contains a lectin on its seeds named WSMoL which modulates several immune characteristics and has shown cardiac safe properties. Here, we tested the hypothesis that WSMoL is able to recover fasting glucose levels and to improve the cardiac left ventricu...
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The aim of the present study was to evaluate the insecticidal activity of ether extract from the lichen Ramalina complanata, and an isolated metabolite (divaricatic acid), against the maize weevil, Sitophilus zeamais. The evaluation of ingestion toxicity was performed using an artificial diet composed of the extract or compound (4 mg/g, 8 mg/g, or...
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Previous studies have characterized a saline extract from Microgramma vacciniifolia rhizome and its lectin (MvRL)‐rich fraction with low acute toxicity. In the present study, we evaluated these preparations for acute toxicity (1,000 mg/kg) and antinociceptive and anti‐inflammatory activities (100–400 mg/kg for the extract and 25–50 mg/kg for the fr...
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Background Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes....
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Cryptococcus is a genus of dimorphic basidiomycete fungi found in the form of yeasts and filaments. Cryptococcosis has as main etiological agents the species Cryptococcus neoformans and Cryptococcus gattii. This disease is considered a public health problem and has becoming more alarming because of the limitations of antimicrobials available to its...
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The measures currently used to minimize the spread of arboviruses, comprising dengue, Chikungunya, and Zika virus, involve controlling the size of population of the mosquito Aedes aegypti. However, the search for formulations containing new insecticides is gaining pace due to reports of mosquito populations showing resistance to commonly used compo...
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Depression and anxiety disorder are the most common mental disorders worldwide and their treatments are combinations of pharmacological and psychotherapeutic approaches. Depression treatment depends largely on a pharmacotherapy that improves the transmission of monoamines in the brain. However, the drugs available have adverse reactions and do not...
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Spondias tuberosa é uma planta medicinal usada para tratar doenças inflamatórias e distúrbios digestivos. Foram investigadas as atividades fitoquímica, antioxidante, fotoprotetora e hemótica dos extratos de acetato de etila dos frutos e ramos. As atividades antioxidantes foram avaliadas pelos ensaios de DPPH, ABTS e fosfomolibdênio. As atividades f...
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Streptomyces hygroscopicus UFPEDA 3370 was fermented in submerged cultivation and the biomass extract was partitioned, obtaining a fraction purified named EB1. After purification of EB1 fraction, nigericin free acid was obtained and identified. Nigericin presented cytotoxic activity against several cancer cell lines, being most active against HL-60...
Chapter
Moringa oleifera Lam. is a tree species with high food, medicinal, and industrial values, being widely cultivated and used in treatment of water for human consumption. The use of M. oleifera is increasing and its commercialization has driven the search for scientifically proven information about safety and therapeutic effectiveness. Natural compoun...
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Tropical neglected diseases (TNDs) are a group of parasitic infections that prevail in tropical and subtropical regions, affecting more than one billion people who live predominantly in developing countries. The poorest and marginalized populations suffer the most because of TNDs. These diseases strongly impact the local economy since billions of d...
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Actinobacteria constitute a group of Gram-positive bacteria found in different ecosystems, such as soil, seawater, human skin as well as lung and gastrointestinal tracts. Secondary metabolites produced by these microorganisms have important biological activities related to beneficial effects on human health and actinobacteria are the prokaryotes of...
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In this study, we evaluated the antitumor activity of a lectin from Microgramma vacciniifolia frond (MvFL) in sarcoma 180-bearing mice. First, the in vitro cytotoxicity of MvFL on sarcoma 180 cells was evaluated. Tumor cells were inoculated into Swiss female mice, and after 7 days, the animals were treated intraperitoneally with phosphate-buffered...
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Egletes viscosa is a plant with therapeutic value due to its antibacterial, antinociceptive and gastroprotective properties. This study aimed to purify, characterize, and evaluate the cytotoxicity of a lectin (EgviL) from the floral capitula of E. viscosa. The lectin was isolated from saline extract through precipitation with ammonium sulfate follo...
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A junção de diversos conceitos e linhas de pesquisas referentes aos microrganismos, suas patogenicidades, as terapias em uso e as emergentes fizeram surgir a coleção intitulada “Fatores de Virulência Microbianos e Terapias Emergentes” que consiste em revisões acadêmicas, pesquisas originais e estudos com aspectos clínicos e que estão diretamente re...
Chapter
A junção de diversos conceitos e linhas de pesquisas referentes aos microrganismos, suas patogenicidades, as terapias em uso e as emergentes fizeram surgir a coleção intitulada “Fatores de Virulência Microbianos e Terapias Emergentes” que consiste em revisões acadêmicas, pesquisas originais e estudos com aspectos clínicos e que estão diretamente re...
Chapter
A junção de diversos conceitos e linhas de pesquisas referentes aos microrganismos, suas patogenicidades, as terapias em uso e as emergentes fizeram surgir a coleção intitulada “Fatores de Virulência Microbianos e Terapias Emergentes” que consiste em revisões acadêmicas, pesquisas originais e estudos com aspectos clínicos e que estão diretamente re...
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Our objectives were to identify genes of the multi-drug efflux system and to evaluate the antimicrobial activities of polypyrrole nanoparticles (PPy-NPs) and aqueous extract of Moringa oleifera against Staphylococcus spp. isolated from dairy farms in Northeast Brazil. Initially, 162 Staphylococcus spp. isolates were subjected to in vitro antimicrob...
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Haemonchus contortus is a hematophagous parasite causing damage to the production of ruminant animals throughout the world. This study evaluated the in vitro effect of proteins from Moringa oleifera (WSMoL - Water Soluble M. oleifera Lectin and cMoL - coagulant M. oleifera Lectin) on the motility of infective larvae and adult male and female worms...
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Microgramma vacciniifolia is broadly used in folk medicine but safety information is unavailable. Therefore, we evaluated the toxicity of a saline extract and a lectin-rich fraction of M. vacciniifolia rhizome. The extract showed hemolytic activity on mice erythrocytes at 1000 μg/mL, whereas the fraction promoted hemolysis (8.57-26.15%) at all test...
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Sitophilus zeamais Motsch. (Coleoptera: Curculionidae) is one of the most common and destructive storage pests in the world and its control depends mostly on the utilization of synthetic pesticides. The hazards of synthetic chemicals to people and the environment, together with the development of resistance by the insects, stimulate the investigati...
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Life-threatening bacterial infections are a major concern in health care services worldwide. This retrospective study aimed to demonstrate genetic and biochemical diversity in isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from a public hospital in Brazil. A total of 63 isolates collected from different sites of infection and hospit...
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Ethnopharmacological relevance Pilosocereus gounellei (xique-xique) is a popular cactus from Caatinga, traditionally used to counter inflammatory processes and indicated as a hypoglycemic agent. Previous studies have shown that mice treated orally with saline extract of P. gounellei stem (containing flavonoids and sugars) showed decreased serum lip...
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The plant Moringa oleifera is used as food and medicine. M. oleifera flowers are source of protein, fiber, and antioxidants, and are used to treat inflammation and tumors. This work evaluated the antitumor activity of the M. oleifera flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice. Swiss female mice were inoculated with sarcoma 180 cel...
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The search for new compounds to control Plutella xylostella, the main pest of Brassica crops, has been stimulated by the environmental impact of the insecticides currently used and reports of resistant populations. This work evaluated the effects of a cladode extract from Opuntia ficus-indica (CE) on the survival and development of P. xylostella, a...
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Carbohydrates perform important physiological functions in eukaryotic and prokaryotic cells. Indeed, alterations in glycan patterns may be associated with disorders. The analysis of these sugars can be reached using nanoprobes composed by lectins associated with fluorescent nanoparticles. This study reports the conjugation of a galactose-binding le...
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During the last decades, there has been an increasing interest in natural compounds for the control of insect pest. The present study evaluated the insecticidal activity of a saline extract of Abarema cochliocarpos bark against Aedes aegypti, Sitophilus zeamais, Nasutitermes corniger, and Fusarium spp. Thin layer chromatography revealed the presenc...
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The mosquito Aedes aegypti L. is a vector transmitting diseases such as dengue, chikungunya and Zika virus fever. The water-soluble lectin from Moringa oleifera Lam. seeds (WSMoL) is larvicidal, ovicidal and can stimulate oviposition in A. aegypti. This study aimed to investigate whether WSMoL could bind to membrane proteins from A. aegypti legs. I...
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Background: Biomphalaria glabrata is an intermediate host for the larvae of Schistosoma mansoni, which is distributed widely in the tropics. B. glabrata control is important to minimize the spread of schistosomiasis and natural compounds have been sought for use against this disease. The Artemia salina bench-top bioassay has been used to investigat...
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The maize weevil Sitophilus zeamais is a pest of remarkable economic importance as it attacks stored grains, processed cereals, and industrial foods. In this study, a water extract (WE) from Moringa oleifera seeds and water-soluble lectin (WSMoL) isolated from it were investigated for insecticidal activity against S. zeamais adults. The insects wer...
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This work evaluated the antioxidant properties and in vivo antinociceptive and anti-inflammatory effects of extracts obtained from fruit peels of Myrciaria floribunda (H. West ex Willd.) O. Berg (Myrtaceae). This plant is popularly known in Brazil as Cambuí or camboim. Different extracts were submitted to comparative analysis to determine the conte...
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Schinus terebinthifolia leaf lectin (SteLL) was reported to be an antimicrobial and antitumor agent. In this work, we evaluated the immunomodulatory activity of SteLL on mice splenocytes and also determined its native molecular mass and putative sequence similarities with plant proteins. The effects of SteLL (12.5 μg/mL) on viability, cytosolic Ca2...
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Pilosocereus gounellei (Cactaceae) is used to treat wounds and inflammation. In this study, we evaluated whether the saline extract from its stem would have genotoxic or anti-genotoxic effects. In the genotoxicity evaluation, mice received the extract (500, 1,000, or 2,000 mg/kg) orally while negative and positive controls were treated with saline...
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Background Moringa oleifera seeds, which are used for water clarification, contain a lectin named WSMoL which has shown in vitro antibacterial and immunomodulatory activity. Due to their nutritional value and therapeutic potential, the leaves and seeds of this tree are eaten in some communities. Some plant lectins are non-toxic to mammals, but othe...
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Acinetobacter baumannii and Pseudomonas aeruginosa are the most relevant Gram-negative bacteria associated with hospital and opportunistic infections. This study aimed to evaluate the dynamics of drug-resistant A. baumannii and P. aeruginosa and biofilm formers from two public hospitals in northeastern Brazil. One hundred isolates (35 from A. bauma...