Paolo Ermacora’s research while affiliated with University of Udine and other places

Late blight caused by the oomycete Phytophthora infestans is a disease of potato and tomato of worldwide relevance and is widespread throughout Europe and the Mediterranean region. While pathogen populations in northern Europe have been sampled and characterized for many years, the genetic structure of populations from southern Europe including Italy, has been less studied. Between 2018 and 2019 we collected 91 samples of P. infestans from potato and tomato crops in Italy, Algeria and Tunisia on FTA cards and genotyped them using 12‐plex microsatellites. These samples were compared to genotypes of P. infestans previously collected within the framework of the EuroBlight network and from published sources. Four clonal lineages were identified: 13_A2 (Blue 13), 2_A1, 23_A1, and 36_A2. Two other isolates collected could not be matched to any currently known clonal lineage. The 13_A2 and 36_A2 lineages were found exclusively in southern Italy and Algeria, while 2_A1 was only found in Algeria. This is the first report of the 36_A2 lineage in Italy. Two isolates from Solanum nigrum were 13_A2 suggesting this weed host could be a reservoir of inoculum. The 23_A1 lineage was found widely on infected tomato crops in Italy and is the same as the lineage US‐23 that is widespread in North America. Differences in genotypes across the country suggests that there may be different sources of introduction into Italy, possibly via infected seed tubers from other countries in Europe, tubers for consumption from North Africa or tomatoes.

Nuts collected from wild accessions of Persian walnut (Juglans regia L.) in the Friuli Venezia Giulia region (North Eastern Italian Alps) were evaluated during 2013–2015. The analyses carried out were mainly on fruit traits, the only morphological traits that could be observed and statistically analysed, being less dependent on the area of sampling, considering geographic features such as altitude, slope, soil, and climate. Such fruit traits proved to be very variable. The nut weight ranged from 2.2 to 17.3 g, the shell thickness from 0.35 to 2.30 mm, the color of kernel skin from light to amber. The fruit nut appearance, evaluated by a panel of consumers, varied from 2.50 to 6.83 in a scale from 0 (very bad) to 10 (very attractive). A multivariate analysis carried out considering traits valuable for breeding and selection, such as nut weight, kernel weight: nut weight ratio, shell thickness, kernel color, and fruit appearance, produced a ranking list, that included, at best, accessions that could be either selected as such for vegetative propagation and distribution to growers or used in breeding programs. A disadvantage of these selections is their terminal bearing habit which is not appreciated by breeders, who prefer the most productive genotypes with lateral bearing habit.

Juglans regia L. is distributed primarily across temperate and subtropical regions of the Northern Hemisphere. During the last glaciation, the species survived in refugial areas that in Europe included the Balkans and the Italian peninsula, two areas joined by a corridor represented by the Friuli Venezia Giulia region, where two germplasm reservoirs met and likely intercrossed during re-colonization after the last glaciation. In this work, two hundred and fifteen wild accessions native to the area were sampled, georeferenced, and genotyped with 20 microsatellite loci selected from the literature. The local accessions of this study displayed moderate genetic diversity with 80 alleles identified. The number of alleles/loci was 4.0 (4.7 alleles for the genomic SSRs (Simple Sequence Repeats) and 2.7 alleles per EST (Expressed Sequence Tag)-derived SSR, on average). An analysis of molecular variance (AMOVA) revealed that most of the molecular diversity was between individuals (nearly 98% of variation explained). The model-based clustering algorithms implemented either in STRUCTURE and GENELAND software revealed two clusters: The first one encompassed most of the samples and showed a great genetic admixture throughout the five sampling areas defined on the base of orographic characteristics of the region. The second cluster represented a small island with three samples traced back to an introduction from Russia at the beginning of the 20th century.

The fundamental purpose of this series is to make known the linked agrobiodiversity to the territory, to human activities, to their impact on nature, all factors that they have affected a precise evolutionary process. We must also emphasize the urgency of this information work because this type of biodiversity is a risk since it is linked to traditional farms with a future really uncertain.