Panyong Mao's research while affiliated with Chinese PLA General Hospital (301 Hospital) and other places

Publications (49)

Article
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SARS-CoV-2 related coronaviruses (SARS-CoV-2r) from Guangdong and Guangxi pangolins have been implicated in the emergence of SARS-CoV-2 and future pandemics. We previously reported the culture of a SARS-CoV-2r GX_P2V from Guangxi pangolins. Here we report the GX_P2V isolate rapidly adapted to Vero cells by acquiring two genomic mutations: an alanin...
Preprint
Full-text available
SARS-CoV-2 related coronaviruses (SARS-CoV-2r) from Guangdong and Guangxi pangolins have been implicated in the emergence of SARS-CoV-2 and future pandemics. We previously reported the culture of a SARS-CoV-2r GX_P2V from Guangxi pangolins. Here we report the GX_P2V isolate rapidly adapted to Vero cells by acquiring two genomic mutations: an alanin...
Article
Full-text available
Background Hematological comparison of coronavirus disease (COVID-19) and other viral pneumonias can provide insights into COVID-19 treatment. Methods In this retrospective case–control single-center study, we compared the data of 126 patients with viral pneumonia during different outbreaks [severe acute respiratory syndrome (SARS) in 2003, influe...
Article
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Zika virus (ZIKV) infection in the human central nervous system (CNS) causes Guillain–Barre syndrome, cerebellum deformity, and other diseases. Astrocytes are immune response cells in the CNS and an important component of the blood–brain barrier. Consequently, any damage to astrocytes facilitates the spread of ZIKV in the CNS. Connective tissue gro...
Article
It is important to identify viruses in animals because most infectious diseases in humans are caused by viruses of zoonotic origin. African green monkey is a widely used non-human primate model in biomedical investigations. In this study, total RNAs were extracted from stool samples of 10 African green monkeys with diarrhea. High-throughput sequenc...
Data
KPC-producing Enterobacteriaceae strains.
Data
Schematic maps of IncX6 plasmids. Genes are denoted by arrows, and the backbone and accessory module regions are highlighted in black and gray, respectively. The innermost circle presents GC-skew [(G-C)/(G+C)], with a window size of 500 bp and a step size of 20 bp. The next-to-innermost circle presents GC content.
Data
Alignment of promoter-proximal regions of blaKPC-2. The 898–354 bp upstream sequences together with the start codon of the blaKPC-2 genes from IncX6 plasmids and Tn6296 are aligned by MUSCLE. Shown are core promoter regions, -35 and -10 elements, transcription starts, Shine-Dalgarno (SD) sequences for ribosome recognition and translation starts.
Data
Major features of ΔTn6296 derivatives compared to Tn6296.
Article
Full-text available
Forty-five KPC-producing Enterobacteriaceae strains were isolated from multiple departments in a Chinese public hospital from 2014 to 2015. Genome sequencing of four representative strains, namely Proteus mirabilis GN2, Serratia marcescens GN26, Morganella morganii GN28, and Klebsiella aerogenes E20, indicated the presence of blaKPC-2-carrying IncX...
Article
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Background Tick-borne encephalitis virus (TBEV) is one of the most important flaviviruses that targets the central nervous system (CNS) and causes encephalitides in humans. Although neuroinflammatory mechanisms may contribute to brain tissue destruction, the induction pathways and potential roles of specific chemokines in TBEV-mediated neurological...
Article
Full-text available
DNA vaccines are simple to produce and can generate strong cellular and humoral immune response, making them attractive vaccine candidates. However, a major shortcoming of DNA vaccines is their poor immunogenicity when administered intramuscularly. Transcutaneous immunization (TCI) via microneedles is a promising alternative delivery route to enhan...
Article
Full-text available
The aim of this study is to summarize and quantify the current evidence on the therapeutic efficacy of cryoablation compared with radiofrequency ablation (RFA) in patients with hepatic malignancies in a meta-analysis. Data were collected by searching PubMed, Scopus, and Cochrane databases for reports published up to May 26, 2015. Studies that repor...
Article
Background: We conducted a systematic review and meta-analysis to determine the association between serum lipid levels and suicidality, as evidence from previous studies has been inconsistent. Methods: We identified relevant studies by searching Medline, Web of Science, EMBASE, and the Cochrane Database of Systematic Reviews (1980 to Dec. 5, 201...
Article
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Studies that investigated the association between socio-economic position (SEP) and obesity in children suggest inconsistent results. The aim of this study is to summarize and quantify the current evidence on SEP and risks of overweight and obesity in children aged 0-15 years. Relevant studies published between 1990 to Sep 4, 2014 were searched in...
Article
Objective To develop an anti-hepatitis E virus (anti-HEV) IgG chemiluminescence assay kit and assess its clinical application. Methods The HEV recombinant antigen was used as coating antigen, horseradish peroxidase (HRP)-conjugated monoclonal anti-human IgG as the secondary antibody, and the luminol chemiluminescent reaction system as a substrate....
Article
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Unlabelled: BACKGROUND AND RATIONALE FOR THE STUDY: To investigate thyroid function in patients with acute-on-chronic liver failure (ACLF) caused by hepatitis B virus infection and to determine whether thyroid hormone levels can be used as prognostic markers for assessing severity and prognosis of ACLF patients. We enrolled 75 patients with ACLF a...
Article
To understand the incidence and death patterns of viral hepatitis in China and provide evidence for the prevention and control of viral hepatitis. The analysis was conducted on the incidence and death data of viral hepatitis published by National Health and Family Planning Commission during 2004-2013. The incidences of viral hepatitis in Guizhou,Yu...
Article
Full-text available
Abstract DNA vaccines are simple to produce and can generate strong cellular and humoral immune response, making them attractive vaccine candidates. However, a major shortcoming of DNA vaccines is their poor immunogenicity when administered intramuscularly. Transcutaneous immunization (TCI) via microneedles is a promising alternative delivery route...
Article
We systematically reviewed the association of omega-3 fatty acids intake with the incidence of dementia and Alzheimer's disease (AD) in this meta-analysis of prospective cohort studies, as evidence from previous studies suggests inconsistent results.Methods We identified relevant studies by searching PubMed, EmBase, and Web of Science databases up...
Article
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Our objective is to develop a rapid and sensitive assay based on magnetic beads to detect the concentration of influenza H3N2. The possibility of using variable domain heavy-chain antibodies (nanobody) as diagnostic tools for influenza H3N2 was investigated. A healthy camel was immunized with inactivated influenza H3N2. A nanobody library of 8 × 10...
Article
This study investigated features and clinical implications of HBV mutations in patients with different clinical manifestations. In total, 516 patients were enrolled in this study, including 131 patients with acute hepatitis B, 239 patients with chronic hepatitis B, and 146 patients with acute-on-chronic liver failure. HBV genotypes and mutations we...
Article
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Enteroviruses can frequently target the human central nervous system to induce a variety of neurological diseases. Although enteroviruses are highly cytolytic, emerging evidence has showed that these viruses can establish persistent infections both in vivo and in vitro. Here, we investigated the susceptibility of three human brain cell lines CCF-ST...
Article
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Vibrio parahaemolyticus AphA and OpaR are the two master quorum sensing (QS) regulators that are abundantly expressed at low cell density (LCD) and high cell density (HCD), respectively, with a feature of reciprocally gradient production of them with transition between LCD and HCD. The type VI secretion system 2 (T6SS2) gene cluster can be assigned...
Data
Primer extension assay for validation of non-polar deletion of opaR or aphA. For complementation of ΔopaR or ΔaphA, a PCR-generated DNA fragment composed of the entire coding region of opaR or aphA, respectively, together with a upstream synthetic ribosome binding site (Table 1), was cloned into between the XbaI and HinDIII sites of pBAD33 vector [...
Article
Vibrio parahaemolyticus AphA and OpaR are the two master regulators of quorum sensing (QS) that are abundantly produced and operate at low cell density (LCD) and high cell density (HCD), respectively, with an outcome of reciprocally gradient production of these two proteins with transition between LCD and HCD. The cpsQ-mfpABC gene cluster is transc...
Article
Infections with bacterial or fungal biofilms have emerged as a major public heath concern because biofilm-growing cells are highly resistant to both antibiotics and host immune defenses. This review focuses on the progress in understanding the mechanisms of biofilm-specific antimicrobial resistance and in developing innovative therapeutic measures...
Article
The clinical use of a bioartificial liver (BAL) device strongly depends on the development of human liver cell lines. The aim of this study was to establish and assess the potential use of the stable HepG2 cell line expressing human augmenter of liver regeneration (hALR). The cDNA encoding hALR protein was inserted into pcDNA3.1 to generate pcDNA3....
Article
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Precise regulation of innate immunity is crucial for maintaining optimal immune responses against infections. Whereas positive regulation of IFN signaling elicits rapid type I IFNs, negative regulation is equally important in preventing the production of superfluous IFNs that can be hazardous to the host. The positive regulators of IFN pathway are...
Article
Influenza has emerged every year but a complete profile of laboratory indices throughout the disease course remains unknown. Clinical data was collected from 28 confirmed cases of the pandemic influenza H1N1 2009. The levels of serum iron (Fe), carbon dioxide combining power (CO2-CP), total complement hemolytic activity (CH50), C-reactive protein (...
Article
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To evaluate vaccine efficacy in protecting against coxsackievirus A16 (CA16), which causes human hand, foot, and mouth disease (HFMD), we established the first neonatal mouse model. In this article, we report data concerning CA16-induced pathological changes, and we demonstrate that anti-CA16 antibody can protect mice against lethal challenge and t...
Article
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Human bocavirus (HBoV) mainly infects young children. Although many infected children suffer from respiratory or gastroenteric tract diseases, an association between HBoV and these diseases is not definite. Because modulation of type I IFN is crucial for viruses to establish efficient replication, in this study, we tested whether HBoV modulates typ...
Article
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The mimotopes of viruses are considered as the good targets for vaccine design. We prepared mimotopes against multiple subtypes of influenza A and evaluate their immune responses in flu virus challenged Balb/c mice. The mimotopes of influenza A including pandemic H1N1, H3N2, H2N2 and H1N1 swine-origin influenza virus were screened by peptide phage...
Article
Full-text available
Enterovirus 71 (EV71), a single, positive-stranded RNA virus, has been regarded as the most important neurotropic enterovirus after the eradication of the poliovirus. EV71 infection can cause hand, foot, and mouth disease or herpangina. Cytokine storm with elevated levels of proinflammatory and inflammatory cytokines, including TNF-α, has been prop...
Article
Lipopolysaccharide (LPS) is suspected to trigger primary biliary cirrhosis (PBC) in susceptible individuals, yet the precise mechanism of its effect in PBC remains largely unknown. The aim of this study was to investigate altered responses to LPS ligand for Toll-like receptors (TLRs) in pathogenesis of PBC in vivo and in vitro. In vivo, we investig...
Article
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To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features. Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) p...
Article
The association of hepatitis B virus (HBV) genotypes and basal core promoter (BCP) and precore (PC) mutations with the clinical characteristics is increasingly recognized. To investigate virologic features and clinical implications of HBV genotypes, BCP and PC mutations between large-size patients with acute hepatitis B (AHB) and chronic hepatitis...
Article
Chronic hepatitis B virus (HBV) infection is an important risk factor for hepatocellular carcinoma (HCC) development in China, while little is known of the genetic susceptibility to hepatocarcinogenesis. The epidermal growth factor (EGF) pathway plays an important role in tumorigenesis, including HCC. EGF polymorphisms are associated with susceptib...
Article
Full-text available
The pathogenesis of SARS coronavirus (CoV) remains poorly understood. In the current study, two recombinant baculovirus were generated to express the spike (S) protein of SARS-like coronavirus (SL-CoV) isolated from bats (vAcBS) and the envelope (E) and membrane (M) proteins of SARS-CoV, respectively. Co-infection of insect cells with these two rec...
Article
Continuous efforts have been made to develop a prophylactic vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, two recombinant baculoviruses, vAc-N and vAc-S, were constructed, which contained the mammalian-cell activate promoter element, human elongation factor 1α-subunit (EF-1α), the human cytomegalovirus (CM...
Article
Continuous efforts have been made to develop a prophylactic vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, two recombinant baculoviruses, vAc-N and vAc-S, were constructed, which contained the mammalian-cell activate promoter element, human elongation factor 1alpha-subunit (EF-1alpha), the human cytomegalov...

Citations

... In this paper, correlations with statistical significance are presented using more complete data available from [43]. Furthermore, data showing the viral titer of pangolin-CoV-2017 in comparison to that of SARS-CoV-2 were not available then [44,45]. Additionally, shell disorder analysis was yet to be performed on the COVID-19related sequence obtained from bats in Laos [7]. ...
... In our study, the total leucocyte count had no impact on the patient outcomes even though previous studies demonstrated increased mortality in COVID-19 patients with a higher leucocyte count [21,28,29]. Non-survivors had a higher neutrophil count compared to the survivor group, although the difference was not statistically significant. ...
... PBVs are widely distributed among vertebrates, invertebrates and environmental samples (Bányai et al., 2008;Bodewes et al., 2013;Ghosh et al., 2009;Ribeiro Silva et al., 2014;Shi et al., 2016b). Although the pathogenicity of PBVs is uncertain, they were traditionally considered as opportunistic enteric pathogens because those viruses have been detected in sporadic cases of diarrhea as well as associated with outbreaks of gastroenteritis in humans and in various animals (Li et al., 2020;Malik et al., 2014;Wilburn et al., 2017). At present, the true host of PBVs is still controversial. ...
... Connective tissue growth factor/Nephroblastoma overexpressed gene family 1 (CCN1), an inducible extracellular matrix protein secreted by reactive astrocytes was upregulated in the PFCw [48]. The kinase, CAMK1, which regulates CCN1 expression and is induced in astrocytes following Zika infection [49], was also correspondingly elevated (Fig. 4E). This gene expression program indicated that astrocyte, microglial, and T cell activation programs were induced in the frontal cortex white matter following SHIV infection. ...
... When compared with pGN26-KPC (the green circle) (Li et al. 2018), pKPC3-SZ (the blue circle) (Du et al. 2016) and pCRE3-KPC (the lilac circle) (Dong et al. 2020), they have lower similarity (< 50% nucleotide identity similarity of the backbone area), but they have almost the same antibiotic resistance gene region carrying blaKPC-2 and belong to transposon Tn6296 and its derivatives (Fig. 2). Compared with pGN26-KPC and pKPC3-SZ, pKPC-2-HENAN1602 has a different backbone, but the ORFs encoding repB, transcriptional regulator, conjugal transfer protein, etc., which maintain the key functions of plasmid replication, conjugation, transport and stability and content in the backbone of the plasmid, certainly demonstrate resemblance. ...
... However, it has been shown that LGTV can be used to induce encephalitis in laboratory mice or rats, when the virus is inoculated intracerebrally or subcutaneously [23,24]. Infection of mice with TBEV strains usually lead to a very high mortality within few days after infection [24][25][26][27], which is not reflecting the clinical situation in humans [27,28]. Using LGTV, in contrast, induces an attenuated form of the disease that allows to assess long-term effects of an infection. ...
... 3C protease is shown to block the NF-κB pathway to proinflammatory cytokine production by targeting the TAK1/TAB1/TAB2/TAB3 complex [72]. EV-A71 2C targets IKKβ and p65 to suppress NF-κB activation [73][74][75]. In addition to viral factors, EV-A71 infection also induces the expression of host microRNA miR-146a, which in turn downregulates the expression of TRAF6 and IRAK1 involved in TLR signaling to type I IFN induction [76]. ...
... Radiofrequency ablation (RFA) is the most clinically verified and used ablation modality, although it has specific limitations. Alternate energy-based technologies using thermal (hot or cold) effect for focal tumor ablation have been recently introduced in clinical use, namely microwave ablation (MWA), cryoablation (CrA), high-intensity focused ultrasound (HIFU), laser therapy, and irreversible electroporation (IRE) [4,[6][7][8][9][10][11][12]. ...
... Mouse primary renal tubular epithelial cells (RTEC) were obtained from Procell Life Science & Technology (Procell) and cultured in epithelial cell medium supplemented with epithelial cell growth supplement (Procell) and penicillin/streptomycin solution. The TBEV Far-Eastern subtype strain WH2012 was isolated and propagated as described previously (32). TBEV European subtype prototypic strain Neudoerfl was derived from a full-length cDNA clone, pTNd/c, which was generously provided by F. X. Heinz (Medical University of Vienna, Austria). ...
... Результаты большинства исследований дают основание считать дислипидемию, в особенности низкий уровень холестерина, фактором риска суицидального поведения [25,26,[35][36][37][38][39][44][45][46]. Метаанализ работ, посвящённых этой проблеме, показал, что у пациентов, страдающих депрессией и совершивших суицидальную попытку, уровень триглицеридов (ТГ), холестерина, липопро-теинов низкой плотности (ЛПНП), липопротеинов высокой плотности (ЛПВП) в плазме крови ниже, чем у депрессивных пациентов, не совершавших парасуицид [43]. D. Lester обнаружил более низкий уровень холестерина в плазме крови парасуицидентов по сравнению с теми, кто не совершал суицидальную попытку, а также показал, что низкий уровень холестерина является фактором риска завершенного суицида [19]. ...