P. Alexyuk's research while affiliated with Almaty Institute of Physics and Technology and other places

Publications (67)

Article
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Here we report the draft complete genome sequence of Escherichia phage vB_EcoM_IntR and Escherichia phage vB_EcoM_PiR isolated from a poultry farm soil sample. Bacteriophage isolation on the model of the pathogenic for birds Escherichia coli was carried out. Sequencing was carried out on the Illumina platform. Phage genomes were assembled using a s...
Article
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Here, we report a metagenomic analysis of koumiss from Kazakhstan. In this study, shotgun metagenomic sequencing of the RNA and DNA viral community was performed.
Article
In 2019-2020, the Coronavirus (CoV) disease 2019 pandemic created a serious challenge for health care systems in several countries worldwide. A cure has not been developed yet and currently used treatment protocols are aimed at relieving clinical symptoms of the disease. This article presents a retrospective review of biologically active compounds...
Article
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Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (...
Article
The ability of the Auricularia auricular-judae extract to suppress the reproduction of the influenza virus was studied. It was shown that the index of the therapeutic activity of the extract is comparable to that of commercial preparations Tamiflu and Remantadine.
Article
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Here, we present a virome analysis of the surface waters of the Small Aral Sea. In this case, shotgun metagenomic sequencing of the RNA and DNA virus community was used.
Article
The high variability of the influenza virus leads to its rapid adaptation to drugs and vaccines, which requires a change in the strategy and tactics against influenza infection. A deeper understanding of the nature of innate and adaptive immunity for influenza infection is required to create more effective means of influenza preventing and treating...
Article
The use of the nanocapsulated adjuvant Sapomax increased the expression of innate immunity genes (H2Q10, Ddx58, Tyk2, Tlr3, Tlr7, and TNF) responsible for the primary recognition of influenza virus, i.e., those belonging to the RLR and TLR families; genes involved in stimulating the production of type I and III IFN and pro-inflammatory cytokines; a...
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Here, we present a metagenomic analysis of the microflora of the surface waters of the Shardara reservoir, the largest artificial reservoir in Southern Kazakhstan, created to meet irrigation and hydropower engineering needs. In this case, shotgun metagenomic sequencing of the microbial community DNA was used.
Article
It is shown that gallic acid, ellagic acid and patuletin have pronounced prophylactic properties that neutralize the development of experimental influenza infection.
Article
It was shown that adjuvant Sapomax effectively stimulates the formation of levels of various classes of immunoglobulins against gene-engineered proteins of the viruses of hepatitis B and C. Moreover, the absence of stimulation of IgG3 levels indicates the absence of allergic manifestations of the developed immunostimulator.
Article
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Here, we describe the complete genome sequence of the Escherichia coli bacteriophage vB_EcoP_PR_Kaz2018, isolated from a water sample. vB_EcoP_PR_Kaz2018 is a linear double-stranded DNA T7-like podophage with a genome of 39,704 bp containing 45 predicted open reading frames (ORFs).
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Avian pathogenic Escherichia coli (APEC) bacteria are one of the main problems of the poultry industry. An effective way to combat colibacillosis is to use a phage preparation that lyses the bacteria. Here, we report the isolation of an E. coli -infecting phage, CEC_KAZ_2018, isolated from soil.
Article
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Numerous studies have shown that immunostimulatory complexes containing Quil-A saponin and various antigens are effective in stimulating the immune response and can be used as vaccine preparations for animals and humans. However, Quil-A saponin possesses toxicity and haemolytic activity. In the present work, a saponin-containing preparation named “...
Article
Studies on the immunostimulating properties of saponins and methoxyflavonoids obtained from plants provides a theoretical basis for development of new immunopotentiators, thereby increasing effectiveness of immunotherapy and prophylaxis of viral infections. Expression of major genes of the immune response were significantly up-regulated upon introd...
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In recent years, there have been a number of reports on the successful use of immunostimulatory complexes with saponins and viral glycoproteins as veterinary vaccines and in clinical trials for human medicine. The saponins Algiox, Sapanox and Pangisan were isolated and purified by HPLC from Allochrusa gypsophiloides, Saponaria officinalis and Gypso...
Article
Newcastle disease virus (NDV) is an important pathogen in poultry. Waterfowl and a number of other avian species serve as the host for NDV. Severity of the disease is variable and infected animals mainly develop respiratory and neurological symptoms. Outbreaks of NDV in poultry are recorded regularly in the Republic of Kazakhstan despite the widesp...
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Viruses are the most abundant biological entities on Earth and can be found in a variety of environments. A high prevalence of viruses in marine and freshwater systems was initially reported by Spencer in 1955, but the ecological significance of viruses is insufficiently known even until the present day. Viruses are known to play a key role in the...
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Full-text available
Cyanobacteria of the genera Synechococcus and Prochlorococcus are an important contributors to photosynthetic productivity in the water ecosystems. The discovery of genes (psbA, psbD) that encode key photosystem II proteins (D1, D2) in the genomes of phages that infect these cyanobacteria suggests new models for the regulation, function and evoluti...
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Full-text available
In this study was determinate the subtypes and prevalence of low-pathogenic influenza A viruses present on the territory of Kazakhstan in 2004-2006. Further was analyzed the HA and NA genes of these isolates in order to obtain a more detailed knowledge about the genetic variation of influenza A viruses in their natural hosts.
Data
GenBank Accession Numbers FJ434373-FJ434380 represent sequences from the 8 segments of Influenza A virus (A/mute swan/Aktau/1460/2006(H5N1)).

Citations

... More in-depth studies have been carried out on the shrinkage of the Aral Sea [22][23][24], the microbiome of the Aral Sea [25][26][27], and the ecological environment of the basin [28][29][30][31][32]. The research on lake sediments has become more abundant, e.g., environmental reconstructions of the salt water, geological structure, etc. through biological indicators [33][34][35]; the use of sedimentary records of titanium changes to restore the wind intensity and frequency characteristics of western Central Asia since the Late Holocene [36]; the ecological risk assessment of potentially toxic elements in the North Aral Sea [16]; etc. ...
... Together with these achievements in understanding of immune molecular mechanisms, immunodiagnosis and immunotherapy, it has become possible to check more accurately how different medicine classes interrelate to the immune system. It is especially important for regulating organs to distinguish toxical side effects of immunomdulating medicines from their useful therapy effects [14]. ...
... The phenolic compounds of this plant are probably responsible for its strong antioxidant activity (11). The root of the plant is used in the prevention and treatment of various diseases such as microbial/viral infections, cancer, skin inflammation, bronchitis, depression, kidney diseases, and gastric and intestinal ulcers (12)(13)(14)(15)(16)(17)(18)(19). Various chemical materials have been identified in this plant, including glycyrrhizin, 18β glycyrrhizic acid, glabrin A and B, or isoflavones, which are associated with biological properties such as antioxidant, antiviral, antimicrobial, anti-cancer, and anti-inflammatory activities (20). ...
... It displays spermicidal activity due to the presence of saponins (Primorac et al., 1985). Moreover, it can be used as an adjuvant for veterinary vaccines (Turmagambetova et al., 2017). Medicinal herbs have historically been applied to various diseases, particularly in Asian countries (Tyler, 2000). ...
... This study indicates that velogenic NDV infection interferes with the intestinal microbiome in quails. The intestinal microflora is relatively stable under common circumstances but is easily influenced by various diseases (Ma et al., 2017).NDV is the causative agent of ND, which is one of the most highly contagious diseases in chickens, and result in severe economic losses to the poultry industry worldwide (Turmagambetova et al., 2017). In Iran, NDV is endemic in different parts of the country, causing enormous losses due to high mortality, sub-optimal production, slaughterhouse condemnation of carcasses, and high prevention and treatment expenses. ...
... They primarily cause infections of the mouth, face, eyes, pharynx, and central nervous system and can be transmitted via skin exposure, oral secretions, and respiratory droplets (Chayavichitsilp et al., 2009;Dockrell, 2003). Although waterborne transmission is not deemed the main route of hepatitis C and human herpesvirus infection, their genomes have been found in wastewater, polluted freshwater (Alexyuk et al., 2017;Corpuz et al., 2020;McCall et al., 2020), and sewage-contaminated aerosols (Brisebois et al., 2018). In addition, the Herpesviridae family is persistent in water environments and aerosols at ambient temperatures (Sobsey and Meschke, 2003), which could be a risk factor for environmental transmission. ...
... 70 wild birds have died from H5N1 epizooty in this region in total (Tabynov, 2008;Kydyrbaev, 2010). The phylogenetic analysis has shown that the virus which has been registered in Mangystau region was different from EMA groups of HPAI H5N1 viruses and had NS2A genotype that was typical for Gs/Gdlike strains (Chervyakova, 2011;Bogoyavlenskiy, 2011). The study of biological properties has shown that the virus had high pathogenic properties (Sayatov, 2007). ...